Transgenic pigs carrying cDNA copies encoding the murine Mx1 protein which confers resistance to influenza virus infection

An important aspect of gene transfer into farm animals is the improvement of disease resistance. The mouse Mx1 protein is known to be sufficient to confer resistance to influenza viruses. Gene constructs containing the mouse Mx1 cDNA controlled by the human metallothionein IIA promoter (hMTIIA::Mx),...

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Veröffentlicht in:Gene Jg. 121; H. 2; S. 263
Hauptverfasser: Müller, M, Brenig, B, Winnacker, E L, Brem, G
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands 16.11.1992
Schlagworte:
Animals
Animals, Genetically Modified
Antiviral Agents
DNA - genetics
Gene Expression
GTP-Binding Proteins
Immunity, Innate
Mice
Myxovirus Resistance Proteins
Orthomyxoviridae Infections - genetics
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - veterinary
Promoter Regions, Genetic
Proteins - genetics
Recombinant Proteins - metabolism
RNA, Messenger - genetics
Swine
ISSN:0378-1119
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Zusammenfassung:An important aspect of gene transfer into farm animals is the improvement of disease resistance. The mouse Mx1 protein is known to be sufficient to confer resistance to influenza viruses. Gene constructs containing the mouse Mx1 cDNA controlled by the human metallothionein IIA promoter (hMTIIA::Mx), the SV40 early enhancer/promoter region (SV40::Mx) and the mouse Mx1 promoter (mMx::Mx) were transferred into pigs. The results of the gene transfer experiments with the hMTIIA::Mx and the SV40::Mx constructs indicate that the permanent high-level synthesis of Mx1 might be deleterious to the organism: the gene transfer efficiency was surprisingly low, and all transgenic piglets born had rearrangements in their transgene copies that abolished protein synthesis. The use of the interferon (IFN)- and virus-inducible mMx::Mx construct resulted in normal gene transfer efficiency. Two transgenic pig lines could be established which expressed IFN-inducible mouse Mx1 mRNA. Extensive protein analysis did not detect mouse Mx1 in IFN-treated transgenic animals.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0378-1119
DOI:10.1016/0378-1119(92)90130-h