Transfection of Kasumi-1 cells with a new type of polymer carriers loaded with miR-155 and antago-miR-155

Previous studies have demonstrated functional roles for microRNAs (miRNAs) in various aspects of normal and malignant hematopoiesis, including lineage commitment, differentiation, apoptosis and maturation. In vivo delivery of naked DNA, oligonucleotides and miRNAs is complicated by their low stabili...

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Bibliographic Details
Published in:Cancer gene therapy Vol. 20; no. 4; pp. 237 - 241
Main Authors: Klimenko, O V, Shtilman, M I
Format: Journal Article
Language:English
Published: New York Nature Publishing Group US 01.04.2013
Nature Publishing Group
Subjects:
631/337/384/331
631/61/2300/1851
631/61/51/391
692/699/67/1990
Apoptosis
Biomedical and Life Sciences
Biomedicine
Biopolymers
Cell Line, Tumor
Cell Shape
Core Binding Factor Alpha 2 Subunit - genetics
Core Binding Factor Alpha 2 Subunit - metabolism
Cytogenetics
Cytology
fms-Like Tyrosine Kinase 3 - genetics
fms-Like Tyrosine Kinase 3 - metabolism
Gene Expression
Gene Therapy
Genetic Therapy
Health aspects
Humans
Leukemia
Luminescent Proteins - biosynthesis
Luminescent Proteins - genetics
MicroRNA
MicroRNAs - genetics
Microscopy, Fluorescence
miRNA
Nanoparticles - chemistry
Octamer Transcription Factor-3 - genetics
Octamer Transcription Factor-3 - metabolism
Oligonucleotides
Oncogene Proteins, Fusion - genetics
Oncogene Proteins, Fusion - metabolism
original-article
Physiological aspects
Platelets
Polymers
Polyvinyls - chemistry
Polyvinyls - metabolism
Proto-Oncogene Proteins c-kit - genetics
Proto-Oncogene Proteins c-kit - metabolism
Red Fluorescent Protein
RNA Interference
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
RUNX1 Translocation Partner 1 Protein
Transfection
Russia
polymer carriers
Kasumi-1
miR-155
platelets
transfection
ISSN:0929-1903, 1476-5500, 1476-5500
Online Access:Get full text
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Description
Summary:Previous studies have demonstrated functional roles for microRNAs (miRNAs) in various aspects of normal and malignant hematopoiesis, including lineage commitment, differentiation, apoptosis and maturation. In vivo delivery of naked DNA, oligonucleotides and miRNAs is complicated by their low stability, rapid degradation and inefficient delivery into target cells. In our experiments, we used a new type of polymer carriers to monitor the effects of miR-155 and antago-miR-155 on the morphology and genetics of Kasumi-1 cells. We obtained platelet-like cells from leukemic cells, and detected the expression of platelet marker genes after transfection with antago-miR-155. Our findings suggest that administration of miR mimics or antago-miRs as therapeutic agents is a desirable goal for future treatment of hematologic malignancies and that polymer-based carriers for the delivery of miR mimics or antago-miRs may provide a solution to the challenges of standard miR delivery approaches.
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ISSN:0929-1903
1476-5500
1476-5500
DOI:10.1038/cgt.2013.11