Transfection of Kasumi-1 cells with a new type of polymer carriers loaded with miR-155 and antago-miR-155

Previous studies have demonstrated functional roles for microRNAs (miRNAs) in various aspects of normal and malignant hematopoiesis, including lineage commitment, differentiation, apoptosis and maturation. In vivo delivery of naked DNA, oligonucleotides and miRNAs is complicated by their low stabili...

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Vydané v:	Cancer gene therapy Ročník 20; číslo 4; s. 237 - 241
Hlavní autori:	Klimenko, O V, Shtilman, M I
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	New York Nature Publishing Group US 01.04.2013 Nature Publishing Group
Predmet:	631/337/384/331 631/61/2300/1851 631/61/51/391 692/699/67/1990 Apoptosis Biomedical and Life Sciences Biomedicine Biopolymers Cell Line, Tumor Cell Shape Core Binding Factor Alpha 2 Subunit - genetics Core Binding Factor Alpha 2 Subunit - metabolism Cytogenetics Cytology fms-Like Tyrosine Kinase 3 - genetics fms-Like Tyrosine Kinase 3 - metabolism Gene Expression Gene Therapy Genetic Therapy Health aspects Humans Leukemia Luminescent Proteins - biosynthesis Luminescent Proteins - genetics MicroRNA MicroRNAs - genetics Microscopy, Fluorescence miRNA Nanoparticles - chemistry Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Oligonucleotides Oncogene Proteins, Fusion - genetics Oncogene Proteins, Fusion - metabolism original-article Physiological aspects Platelets Polymers Polyvinyls - chemistry Polyvinyls - metabolism Proto-Oncogene Proteins c-kit - genetics Proto-Oncogene Proteins c-kit - metabolism Red Fluorescent Protein RNA Interference RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism RUNX1 Translocation Partner 1 Protein Transfection Russia polymer carriers Kasumi-1 miR-155 platelets transfection
ISSN:	0929-1903, 1476-5500, 1476-5500
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Shrnutí:	Previous studies have demonstrated functional roles for microRNAs (miRNAs) in various aspects of normal and malignant hematopoiesis, including lineage commitment, differentiation, apoptosis and maturation. In vivo delivery of naked DNA, oligonucleotides and miRNAs is complicated by their low stability, rapid degradation and inefficient delivery into target cells. In our experiments, we used a new type of polymer carriers to monitor the effects of miR-155 and antago-miR-155 on the morphology and genetics of Kasumi-1 cells. We obtained platelet-like cells from leukemic cells, and detected the expression of platelet marker genes after transfection with antago-miR-155. Our findings suggest that administration of miR mimics or antago-miRs as therapeutic agents is a desirable goal for future treatment of hematologic malignancies and that polymer-based carriers for the delivery of miR mimics or antago-miRs may provide a solution to the challenges of standard miR delivery approaches.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0929-1903 1476-5500 1476-5500
DOI:	10.1038/cgt.2013.11