Persistence of viral RNA segments in the central nervous system of mice after recovery from acute influenza A virus infection

One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the ce...

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Vydané v:Veterinary Microbiology Ročník 97; číslo 3; s. 259 - 268
Hlavní autori: Park, C.H, Matsuda, K, Sunden, Y, Ninomiya, A, Takada, A, Ito, H, Kimura, T, Ochiai, K, Kida, H, Umemura, T
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Amsterdam Elsevier B.V 30.12.2003
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Elsevier Science
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ISSN:0378-1135, 1873-2542
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Abstract One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the central nervous system (CNS) of recovered mice, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods were performed. Histopathologically, mild interstitial pneumonia and non-suppurative encephalomyelitis restricted to the basal part of the frontal lobe of the cerebrum, brain stem and thoracic spinal cord were observed in BALB/c mice until 40 PID. Small amounts of viral antigens were detected in the brain and spinal cord and some viral RNA segments (NA, NP, M, PA, HA, NS, PB1) were intermittently detected in the CNS until 48 PID. Immunosuppression of these mice by dexamethazone (DEX) treatment did not increase the frequency of detection of the lesions, viral antigens or genomes. These findings suggest that viral genomes of neurovirulent influenza virus persist with restricted transcriptive activity in the CNS of the mice even after clinical recovery from the infection.
AbstractList One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the central nervous system (CNS) of recovered mice, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods were performed. Histopathologically, mild interstitial pneumonia and non-suppurative encephalomyelitis restricted to the basal part of the frontal lobe of the cerebrum, brain stem and thoracic spinal cord were observed in BALB/c mice until 40 PID. Small amounts of viral antigens were detected in the brain and spinal cord and some viral RNA segments (NA, NP, M, PA, HA, NS, PB1) were intermittently detected in the CNS until 48 PID. Immunosuppression of these mice by dexamethazone (DEX) treatment did not increase the frequency of detection of the lesions, viral antigens or genomes. These findings suggest that viral genomes of neurovirulent influenza virus persist with restricted transcriptive activity in the CNS of the mice even after clinical recovery from the infection.
One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the central nervous system (CNS) of recovered mice, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods were performed. Histopathologically, mild interstitial pneumonia and non-suppurative encephalomyelitis restricted to the basal part of the frontal lobe of the cerebrum, brain stem and thoracic spinal cord were observed in BALB/c mice until 40 PID. Small amounts of viral antigens were detected in the brain and spinal cord and some viral RNA segments (NA, NP, M, PA, HA, NS, PB1) were intermittently detected in the CNS until 48 PID. Immunosuppression of these mice by dexamethazone (DEX) treatment did not increase the frequency of detection of the lesions, viral antigens or genomes. These findings suggest that viral genomes of neurovirulent influenza virus persist with restricted transcriptive activity in the CNS of the mice even after clinical recovery from the infection.One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the central nervous system (CNS) of recovered mice, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods were performed. Histopathologically, mild interstitial pneumonia and non-suppurative encephalomyelitis restricted to the basal part of the frontal lobe of the cerebrum, brain stem and thoracic spinal cord were observed in BALB/c mice until 40 PID. Small amounts of viral antigens were detected in the brain and spinal cord and some viral RNA segments (NA, NP, M, PA, HA, NS, PB1) were intermittently detected in the CNS until 48 PID. Immunosuppression of these mice by dexamethazone (DEX) treatment did not increase the frequency of detection of the lesions, viral antigens or genomes. These findings suggest that viral genomes of neurovirulent influenza virus persist with restricted transcriptive activity in the CNS of the mice even after clinical recovery from the infection.
Author Umemura, T
Park, C.H
Kimura, T
Ochiai, K
Matsuda, K
Ito, H
Kida, H
Takada, A
Sunden, Y
Ninomiya, A
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Keywords BALB/c mice
CNS
Influenza virus
Persistent infection
Microbiology
Acute
Rodentia
Central nervous system
Orthomyxoviridae
Influenzavirus
Infection
Virus
Vertebrata
Influenzavirus A
Mammalia
Mouse
Influenza A virus
Viral disease
Influenza A
Veterinary
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Snippet One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16...
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SubjectTerms Animals
BALB/c mice
Biological and medical sciences
Brain
Brain - pathology
Brain - virology
brain stem
Central Nervous System
Central Nervous System - pathology
Central Nervous System - virology
cerebrum
CNS
Disease Models, Animal
encephalitis
Female
Fundamental and applied biological sciences. Psychology
genome
histopathology
Immunohistochemistry
Influenza A virus
Influenza A virus - genetics
Influenza A virus - isolation & purification
Influenza virus
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Orthomyxoviridae Infections
Orthomyxoviridae Infections - mortality
Orthomyxoviridae Infections - pathology
Orthomyxoviridae Infections - virology
Persistent infection
pneumonia
Random Allocation
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA
RNA, Viral
RNA, Viral - analysis
Specific Pathogen-Free Organisms
spinal cord
viral antigens
Virology
Title Persistence of viral RNA segments in the central nervous system of mice after recovery from acute influenza A virus infection
URI https://dx.doi.org/10.1016/j.vetmic.2003.10.001
https://cir.nii.ac.jp/crid/1871428067675610368
https://www.ncbi.nlm.nih.gov/pubmed/14654295
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