Persistence of viral RNA segments in the central nervous system of mice after recovery from acute influenza A virus infection

One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the ce...

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Veröffentlicht in:	Veterinary Microbiology Jg. 97; H. 3; S. 259 - 268
Hauptverfasser:	Park, C.H, Matsuda, K, Sunden, Y, Ninomiya, A, Takada, A, Ito, H, Kimura, T, Ochiai, K, Kida, H, Umemura, T
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Amsterdam Elsevier B.V 30.12.2003 Elsevier BV Elsevier Science
Schlagworte:	Animals BALB/c mice Biological and medical sciences Brain Brain - pathology Brain - virology brain stem Central Nervous System Central Nervous System - pathology Central Nervous System - virology cerebrum CNS Disease Models, Animal encephalitis Female Fundamental and applied biological sciences. Psychology genome histopathology Immunohistochemistry Influenza A virus Influenza A virus - genetics Influenza A virus - isolation & purification Influenza virus Mice Mice, Inbred BALB C Microbiology Miscellaneous Orthomyxoviridae Infections Orthomyxoviridae Infections - mortality Orthomyxoviridae Infections - pathology Orthomyxoviridae Infections - virology Persistent infection pneumonia Random Allocation Reverse Transcriptase Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA RNA, Viral RNA, Viral - analysis Specific Pathogen-Free Organisms spinal cord viral antigens Virology BALB/c mice CNS Influenza virus Persistent infection Microbiology Acute Rodentia Central nervous system Orthomyxoviridae Influenzavirus Infection Virus Vertebrata Influenzavirus A Mammalia Mouse Influenza A virus Viral disease Influenza A Veterinary
ISSN:	0378-1135, 1873-2542
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Zusammenfassung:	One-hundred thirty-seven BALB/c mice were intranasally inoculated with neurotropic avian influenza A virus (H5N3). Thirty-nine of these mice died within 16 days post-inoculation (PID) and 98 of the mice recovered from the infection. To investigate whether viral antigens and genomes persist in the central nervous system (CNS) of recovered mice, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods were performed. Histopathologically, mild interstitial pneumonia and non-suppurative encephalomyelitis restricted to the basal part of the frontal lobe of the cerebrum, brain stem and thoracic spinal cord were observed in BALB/c mice until 40 PID. Small amounts of viral antigens were detected in the brain and spinal cord and some viral RNA segments (NA, NP, M, PA, HA, NS, PB1) were intermittently detected in the CNS until 48 PID. Immunosuppression of these mice by dexamethazone (DEX) treatment did not increase the frequency of detection of the lesions, viral antigens or genomes. These findings suggest that viral genomes of neurovirulent influenza virus persist with restricted transcriptive activity in the CNS of the mice even after clinical recovery from the infection.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2003.10.001