A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae ) exhibits extensive genomic sequence divers...
Uložené v:
| Vydané v: | eLife Ročník 9 |
|---|---|
| Hlavní autori: | , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
England
eLife Science Publications, Ltd
21.10.2020
eLife Sciences Publications, Ltd eLife Sciences Publications Ltd |
| Predmet: | |
| ISSN: | 2050-084X, 2050-084X |
| On-line prístup: | Získať plný text |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV,
Nairoviridae
) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells.
Crimean-Congo hemorrhagic fever (CCHF) is caused by infection with a virus spread by ticks in Europe, Africa and Asia. It can cause severe disease in humans, including high fevers and bleeding. How deadly CCHF is varies with between 5% to 80% of those infected dying. Scientists suspect genetic differences in various strains of the virus may account for the differences in death rates, but they do not know the exact mutations that make the CCHF virus more or less deadly.
To learn more, scientists have sorted strains of CCHF virus into different groups based on how similar they are genetically. One group called Europe 2 infects many people in the Balkans, but it rarely causes illness. In fact, only two mild cases of illness have been associated with Europe 2 strains, while other CCHF virus strains circulating in this region have caused thousands of more severe illnesses.
Now, Hua et al. identified a mutation in one Europe 2 strain of the CCHF virus that may explain why this subgroup of viruses rarely causes severe human disease. The researchers collected a strain of CCHF virus from infected ticks found in Bulgaria and sequenced its genome. They named the virus strain Malko Tarnovo. Through a series of experiments, Hua et al. showed that the Malko Tarnovo strain very efficiently infects tick cells but not human cells. A single amino acid change in the genetic sequence of the virus appears to make the virus less able to infect human cells. The mutation prevents a protein on the surface of the virus from fusing with human cells, an essential step in infection.
This may explain why this strain and others in the Europe 2 group do not cause severe human disease. Hua et al. also demonstrate the importance of studying viruses in the animals that spread them. By studying the CCHF virus in ticks, scientists may be able to learn more about how viruses evolve to infect new species, which may help scientists prevent future pandemics. |
|---|---|
| AbstractList | Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. eLife digest Crimean-Congo hemorrhagic fever (CCHF) is caused by infection with a virus spread by ticks in Europe, Africa and Asia. It can cause severe disease in humans, including high fevers and bleeding. How deadly CCHF is varies with between 5% to 80% of those infected dying. Scientists suspect genetic differences in various strains of the virus may account for the differences in death rates, but they do not know the exact mutations that make the CCHF virus more or less deadly. To learn more, scientists have sorted strains of CCHF virus into different groups based on how similar they are genetically. One group called Europe 2 infects many people in the Balkans, but it rarely causes illness. In fact, only two mild cases of illness have been associated with Europe 2 strains, while other CCHF virus strains circulating in this region have caused thousands of more severe illnesses. Now, Hua et al. identified a mutation in one Europe 2 strain of the CCHF virus that may explain why this subgroup of viruses rarely causes severe human disease. The researchers collected a strain of CCHF virus from infected ticks found in Bulgaria and sequenced its genome. They named the virus strain Malko Tarnovo. Through a series of experiments, Hua et al. showed that the Malko Tarnovo strain very efficiently infects tick cells but not human cells. A single amino acid change in the genetic sequence of the virus appears to make the virus less able to infect human cells. The mutation prevents a protein on the surface of the virus from fusing with human cells, an essential step in infection. This may explain why this strain and others in the Europe 2 group do not cause severe human disease. Hua et al. also demonstrate the importance of studying viruses in the animals that spread them. By studying the CCHF virus in ticks, scientists may be able to learn more about how viruses evolve to infect new species, which may help scientists prevent future pandemics. Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. Crimean-Congo hemorrhagic fever (CCHF) is caused by infection with a virus spread by ticks in Europe, Africa and Asia. It can cause severe disease in humans, including high fevers and bleeding. How deadly CCHF is varies with between 5% to 80% of those infected dying. Scientists suspect genetic differences in various strains of the virus may account for the differences in death rates, but they do not know the exact mutations that make the CCHF virus more or less deadly. To learn more, scientists have sorted strains of CCHF virus into different groups based on how similar they are genetically. One group called Europe 2 infects many people in the Balkans, but it rarely causes illness. In fact, only two mild cases of illness have been associated with Europe 2 strains, while other CCHF virus strains circulating in this region have caused thousands of more severe illnesses. Now, Hua et al. identified a mutation in one Europe 2 strain of the CCHF virus that may explain why this subgroup of viruses rarely causes severe human disease. The researchers collected a strain of CCHF virus from infected ticks found in Bulgaria and sequenced its genome. They named the virus strain Malko Tarnovo. Through a series of experiments, Hua et al. showed that the Malko Tarnovo strain very efficiently infects tick cells but not human cells. A single amino acid change in the genetic sequence of the virus appears to make the virus less able to infect human cells. The mutation prevents a protein on the surface of the virus from fusing with human cells, an essential step in infection. This may explain why this strain and others in the Europe 2 group do not cause severe human disease. Hua et al. also demonstrate the importance of studying viruses in the animals that spread them. By studying the CCHF virus in ticks, scientists may be able to learn more about how viruses evolve to infect new species, which may help scientists prevent future pandemics. Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, ) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells.Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae ) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. Crimean-Congo hemorrhagic fever (CCHF) is caused by infection with a virus spread by ticks in Europe, Africa and Asia. It can cause severe disease in humans, including high fevers and bleeding. How deadly CCHF is varies with between 5% to 80% of those infected dying. Scientists suspect genetic differences in various strains of the virus may account for the differences in death rates, but they do not know the exact mutations that make the CCHF virus more or less deadly. To learn more, scientists have sorted strains of CCHF virus into different groups based on how similar they are genetically. One group called Europe 2 infects many people in the Balkans, but it rarely causes illness. In fact, only two mild cases of illness have been associated with Europe 2 strains, while other CCHF virus strains circulating in this region have caused thousands of more severe illnesses. Now, Hua et al. identified a mutation in one Europe 2 strain of the CCHF virus that may explain why this subgroup of viruses rarely causes severe human disease. The researchers collected a strain of CCHF virus from infected ticks found in Bulgaria and sequenced its genome. They named the virus strain Malko Tarnovo. Through a series of experiments, Hua et al. showed that the Malko Tarnovo strain very efficiently infects tick cells but not human cells. A single amino acid change in the genetic sequence of the virus appears to make the virus less able to infect human cells. The mutation prevents a protein on the surface of the virus from fusing with human cells, an essential step in infection. This may explain why this strain and others in the Europe 2 group do not cause severe human disease. Hua et al. also demonstrate the importance of studying viruses in the animals that spread them. By studying the CCHF virus in ticks, scientists may be able to learn more about how viruses evolve to infect new species, which may help scientists prevent future pandemics. |
| Audience | Academic |
| Author | Marklewitz, Marco Drosten, Christian Nichol, Stuart T Spiropoulou, Christina Kopp, Anne Hua, Brian L Scholte, Florine EM Junglen, Sandra Bergeron, Éric Ohlendorf, Valerie |
| Author_xml | – sequence: 1 givenname: Brian L orcidid: 0000-0002-7580-3399 surname: Hua fullname: Hua, Brian L organization: Centers for Disease Control and Prevention, Atlanta, United States – sequence: 2 givenname: Florine EM orcidid: 0000-0003-2110-3087 surname: Scholte fullname: Scholte, Florine EM organization: Centers for Disease Control and Prevention, Atlanta, United States – sequence: 3 givenname: Valerie surname: Ohlendorf fullname: Ohlendorf, Valerie organization: Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany, German Center for Infection Research (DZIF), Berlin, Germany – sequence: 4 givenname: Anne surname: Kopp fullname: Kopp, Anne organization: Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany, German Center for Infection Research (DZIF), Berlin, Germany – sequence: 5 givenname: Marco orcidid: 0000-0003-1828-8770 surname: Marklewitz fullname: Marklewitz, Marco organization: Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany, German Center for Infection Research (DZIF), Berlin, Germany – sequence: 6 givenname: Christian surname: Drosten fullname: Drosten, Christian organization: Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany, German Center for Infection Research (DZIF), Berlin, Germany – sequence: 7 givenname: Stuart T surname: Nichol fullname: Nichol, Stuart T organization: Centers for Disease Control and Prevention, Atlanta, United States – sequence: 8 givenname: Christina orcidid: 0000-0001-8406-3161 surname: Spiropoulou fullname: Spiropoulou, Christina organization: Centers for Disease Control and Prevention, Atlanta, United States – sequence: 9 givenname: Sandra surname: Junglen fullname: Junglen, Sandra organization: Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany, German Center for Infection Research (DZIF), Berlin, Germany – sequence: 10 givenname: Éric orcidid: 0000-0003-3398-8628 surname: Bergeron fullname: Bergeron, Éric organization: Centers for Disease Control and Prevention, Atlanta, United States |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33084573$$D View this record in MEDLINE/PubMed |
| BookMark | eNptkk2L2zAQhk3Z0t1u99R7MfTSUpJKtmxZl0II_QgECv2A3oQsjRxtbSkr2aH77ztJtsumrH2QPPPMy8z4fZ6d-eAhy15SMudVxd7D2lmYV0QI8SS7KEhFZqRhv84e3M-zq5SuCT6cNQ0Vz7LzssRExcuL7GaRJ-e7HvJhGtXogs-dz5fRDaD8bBl8F_INDCHGjeqczi3sIOY7F6eUG5d0wE8w-5rR6d8pd8NWuYint6BHt3Pj7T65mQblcw19n15kT63qE1zdnZfZz08ffyy_zNZfP6-Wi_VMV6wZZ9YwQllpBFBraFEVVtQt0QRaUVeFAUNqai1VJSVF22AUSt5URau5ICDAlJfZ6qhrgrqWW5xIxVsZlJOHQIidVBGb7kEWvNCtrTkRrWatso1lFdNK1KQUrCQKtT4ctbZTO4DR4Meo-hPR04x3G9mFneTYK6McBd7cCcRwM0Ea5YDLw3UoD2FKsmBVITj-UYHo6yPaKWwN9xhQUe9xuagZTssbViA1f4TC18DgNHrEOoyfFLw9KUBmhD9jp6aU5Or7t1P21cNx7-f8ZxsE3h0BHUNKEew9Qonc-1IefCkPvkSa_kdrd_Qa9uz6R2v-AsuZ5mc |
| CitedBy_id | crossref_primary_10_1038_s41579_023_00871_9 crossref_primary_10_18332_popmed_172259 crossref_primary_10_1002_hsr2_2209 crossref_primary_10_1016_j_antiviral_2022_105401 crossref_primary_10_1016_j_antiviral_2024_105844 crossref_primary_10_1186_s12985_023_02089_w crossref_primary_10_1186_s12879_025_10728_6 crossref_primary_10_3389_fmicb_2023_1185829 crossref_primary_10_3390_v16101547 crossref_primary_10_1007_s40475_021_00235_4 crossref_primary_10_3390_v17081054 crossref_primary_10_3390_v15091891 crossref_primary_10_3390_microorganisms9091907 crossref_primary_10_3390_ijms24076411 crossref_primary_10_1128_jvi_00599_22 |
| Cites_doi | 10.1089/vbz.2017.2190 10.2307/3282757 10.3390/v8040106 10.3201/eid1701.100073 10.1038/nmeth.1701 10.1016/j.ttbdis.2018.02.024 10.1016/j.antiviral.2017.08.014 10.1016/S0022-2836(05)80360-2 10.1038/srep35819 10.1186/s13071-017-2279-1 10.1016/j.ijid.2009.07.017 10.1016/j.ttbdis.2014.04.008 10.1016/j.meegid.2017.08.017 10.1016/j.jcpa.2003.12.002 10.1128/JVI.01859-09 10.1016/j.bbrc.2011.06.109 10.1016/j.antiviral.2013.07.006 10.1371/journal.ppat.1004879 10.1016/j.parepi.2016.08.002 10.1371/journal.pntd.0004259 10.1016/j.ttbdis.2013.04.003 10.7883/yoken.JJID.2015.533 10.1016/j.ttbdis.2016.05.010 10.1128/JVI.00752-06 10.1016/j.ijid.2013.07.015 10.1186/1471-2334-9-90 10.2307/3283188 10.1371/journal.pntd.0003168 10.1016/j.antiviral.2017.05.010 10.1016/j.antiviral.2004.08.001 10.1111/j.1469-0691.2011.03718.x 10.1016/j.antiviral.2017.10.008 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2020 eLife Science Publications, Ltd. |
| Copyright_xml | – notice: COPYRIGHT 2020 eLife Science Publications, Ltd. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM ISR 7X8 5PM DOA |
| DOI | 10.7554/eLife.50999 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Science MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic CrossRef |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2050-084X |
| ExternalDocumentID | oai_doaj_org_article_272cbf6709bc4baf8f454ca96039430a PMC7652417 A641027842 33084573 10_7554_eLife_50999 |
| Genre | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S Journal Article |
| GeographicLocations | Europe |
| GeographicLocations_xml | – name: Europe |
| GrantInformation_xml | – fundername: NIAID NIH HHS grantid: R01 AI109008 – fundername: NIAID NIH HHS grantid: R01 AI151006 – fundername: ; – fundername: ; grantid: 01KI1716 – fundername: ; grantid: R01AI109008 – fundername: ; grantid: TTU 01.801 |
| GroupedDBID | 53G 5VS 7X7 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAKDD AAYXX ABUWG ACGFO ACGOD ACPRK ADBBV ADRAZ AENEX AFFHD AFKRA AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU CITATION DIK DWQXO EMOBN FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HMCUK HYE IAO IEA IHR INH INR ISR ITC KQ8 LK8 M1P M2P M48 M7P M~E NQS OK1 PGMZT PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO RHI RNS RPM UKHRP ALIPV CGR CUY CVF ECM EIF NPM 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c548t-fd40143d9e1fd1252f96b0c0eb9652ded061ff1a3102b8eb9e37852bc790e9ed3 |
| IEDL.DBID | DOA |
| ISICitedReferencesCount | 15 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000589970500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2050-084X |
| IngestDate | Fri Oct 03 12:52:15 EDT 2025 Tue Nov 04 01:46:59 EST 2025 Thu Sep 04 14:23:54 EDT 2025 Tue Nov 11 10:19:54 EST 2025 Tue Nov 04 17:49:12 EST 2025 Thu Nov 13 14:22:45 EST 2025 Mon Jul 21 06:01:45 EDT 2025 Sat Nov 29 06:23:54 EST 2025 Tue Nov 18 21:35:42 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | nairoviridae tick-borne virus phylogenetic infectious disease microbiology virus pathogenesis congo hemorrhagic fever virus virus membrane fusion |
| Language | English |
| License | This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c548t-fd40143d9e1fd1252f96b0c0eb9652ded061ff1a3102b8eb9e37852bc790e9ed3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
| ORCID | 0000-0002-7580-3399 0000-0003-3398-8628 0000-0003-1828-8770 0000-0001-8406-3161 0000-0003-2110-3087 |
| OpenAccessLink | https://doaj.org/article/272cbf6709bc4baf8f454ca96039430a |
| PMID | 33084573 |
| PQID | 2452977559 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_272cbf6709bc4baf8f454ca96039430a pubmedcentral_primary_oai_pubmedcentral_nih_gov_7652417 proquest_miscellaneous_2452977559 gale_infotracmisc_A641027842 gale_infotracacademiconefile_A641027842 gale_incontextgauss_ISR_A641027842 pubmed_primary_33084573 crossref_primary_10_7554_eLife_50999 crossref_citationtrail_10_7554_eLife_50999 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-10-21 |
| PublicationDateYYYYMMDD | 2020-10-21 |
| PublicationDate_xml | – month: 10 year: 2020 text: 2020-10-21 day: 21 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | eLife |
| PublicationTitleAlternate | Elife |
| PublicationYear | 2020 |
| Publisher | eLife Science Publications, Ltd eLife Sciences Publications, Ltd eLife Sciences Publications Ltd |
| Publisher_xml | – name: eLife Science Publications, Ltd – name: eLife Sciences Publications, Ltd – name: eLife Sciences Publications Ltd |
| References | Papa (bib16) 2011; 17 Midilli (bib13) 2009; 9 Sargianou (bib25) 2013; 17 Altschul (bib1) 1990; 215 Bergeron (bib7) 2015; 11 Zivcec (bib35) 2016; 8 Papa (bib17) 2013; 4 Salehi-Vaziri (bib24) 2016; 69 Babos (bib2) 1964 Panayotova (bib15) 2016; 7 Sherifi (bib26) 2014; 8 Zivcec (bib36) 2017; 146 Papa (bib21) 2018; 9 Bell-Sakyi (bib3) 1991; 77 Petersen (bib23) 2011; 8 Papa (bib18) 2014; 5 Gargili (bib12) 2017; 144 Cajimat (bib8) 2017; 17 Whitehouse (bib31) 2004; 64 Bell-Sakyi (bib4) 2004; 130 Sidira (bib28) 2013; 17 Walker (bib29) 2014 Zivcec (bib34) 2015; 9 Xia (bib32) 2016; 6 Elevli (bib11) 2010; 14 Suppl 3 Xiao (bib33) 2011; 411 Papa (bib20) 2017; 54 Bente (bib5) 2013; 100 Sidira (bib27) 2012; 18 Dinçer (bib10) 2017; 10 Munderloh (bib14) 1994; 80 Welch (bib30) 2017; 147 Papadopoulos (bib22) 1980 Deyde (bib9) 2006; 80 Papa (bib19) 2016; 1 Bergeron (bib6) 2010; 84 |
| References_xml | – volume: 17 start-page: 714 year: 2017 ident: bib8 article-title: Genomic characterization of Crimean-Congo hemorrhagic fever virus in Hyalomma tick from Spain, 2014 publication-title: Vector-Borne and Zoonotic Diseases doi: 10.1089/vbz.2017.2190 – volume: 77 start-page: 1006 year: 1991 ident: bib3 article-title: Continuous cell lines from the tick Hyalomma anatolicum anatolicum publication-title: The Journal of Parasitology doi: 10.2307/3282757 – volume: 8 year: 2016 ident: bib35 article-title: Molecular insights into Crimean-Congo hemorrhagic fever virus publication-title: Viruses doi: 10.3390/v8040106 – volume-title: Ticks of Domestic Animals in Africa: A Guide to Identification of Species year: 2014 ident: bib29 – volume: 17 start-page: 141 year: 2011 ident: bib16 article-title: Crimean-Congo hemorrhagic fever virus, northeastern greece publication-title: Emerging Infectious Diseases doi: 10.3201/eid1701.100073 – volume: 8 start-page: 785 year: 2011 ident: bib23 article-title: SignalP 4.0: discriminating signal peptides from transmembrane regions publication-title: Nature Methods doi: 10.1038/nmeth.1701 – volume: 9 start-page: 788 year: 2018 ident: bib21 article-title: Isolation and whole-genome sequencing of a Crimean-Congo hemorrhagic fever virus strain, Greece publication-title: Ticks and Tick-Borne Diseases doi: 10.1016/j.ttbdis.2018.02.024 – volume: 146 start-page: 112 year: 2017 ident: bib36 article-title: Identification of broadly neutralizing monoclonal antibodies against Crimean-Congo hemorrhagic fever virus publication-title: Antiviral Research doi: 10.1016/j.antiviral.2017.08.014 – volume: 215 start-page: 403 year: 1990 ident: bib1 article-title: Basic local alignment search tool publication-title: Journal of Molecular Biology doi: 10.1016/S0022-2836(05)80360-2 – volume-title: Crimean-Congo Hemorrhagic Fever (CCHFV) in Greece: Isolation of the Virus From Rhipicephalous Bursa Ticks and a Preliminary Serological Survey year: 1980 ident: bib22 – volume: 6 year: 2016 ident: bib32 article-title: Transstadial transmission and Long-term association of Crimean-Congo hemorrhagic fever virus in ticks shapes genome plasticity publication-title: Scientific Reports doi: 10.1038/srep35819 – volume: 17 start-page: 322 year: 2013 ident: bib28 article-title: Prevalence of Crimean-Congo hemorrhagic fever virus antibodies in greek residents in the area where the AP92 strain was isolated publication-title: Hippokratia – volume: 10 year: 2017 ident: bib10 article-title: Generic amplification and next generation sequencing reveal Crimean-Congo hemorrhagic fever virus AP92-like strain and distinct tick Phleboviruses in Anatolia, Turkey publication-title: Parasites & Vectors doi: 10.1186/s13071-017-2279-1 – volume: 14 Suppl 3 start-page: e213 year: 2010 ident: bib11 article-title: A newly identified Crimean-Congo hemorrhagic fever virus strain in Turkey publication-title: International Journal of Infectious Diseases doi: 10.1016/j.ijid.2009.07.017 – volume: 5 start-page: 590 year: 2014 ident: bib18 article-title: A novel AP92-like Crimean-Congo hemorrhagic fever virus strain, Greece publication-title: Ticks and Tick-Borne Diseases doi: 10.1016/j.ttbdis.2014.04.008 – volume: 54 start-page: 496 year: 2017 ident: bib20 article-title: Crimean-Congo hemorrhagic fever virus in ticks collected from livestock in Albania publication-title: Infection, Genetics and Evolution doi: 10.1016/j.meegid.2017.08.017 – volume: 130 start-page: 285 year: 2004 ident: bib4 article-title: Ehrlichia ruminantium grows in cell lines from four ixodid tick genera publication-title: Journal of Comparative Pathology doi: 10.1016/j.jcpa.2003.12.002 – volume: 84 start-page: 216 year: 2010 ident: bib6 article-title: Crimean-Congo hemorrhagic fever virus-encoded ovarian tumor protease activity is dispensable for virus RNA polymerase function publication-title: Journal of Virology doi: 10.1128/JVI.01859-09 – volume: 411 start-page: 253 year: 2011 ident: bib33 article-title: Identification of a putative Crimean-Congo hemorrhagic fever virus entry factor publication-title: Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2011.06.109 – volume: 100 start-page: 159 year: 2013 ident: bib5 article-title: Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity publication-title: Antiviral Research doi: 10.1016/j.antiviral.2013.07.006 – volume-title: Die Zeckenfauna Mitteleuropas year: 1964 ident: bib2 – volume: 11 year: 2015 ident: bib7 article-title: Recovery of recombinant crimean Congo hemorrhagic fever virus reveals a function for Non-structural glycoproteins cleavage by furin publication-title: PLOS Pathogens doi: 10.1371/journal.ppat.1004879 – volume: 1 start-page: 211 year: 2016 ident: bib19 article-title: Spatial cluster analysis of Crimean-Congo hemorrhagic fever virus seroprevalence in humans, Greece publication-title: Parasite Epidemiology and Control doi: 10.1016/j.parepi.2016.08.002 – volume: 9 year: 2015 ident: bib34 article-title: Assessment of inhibitors of pathogenic Crimean-Congo hemorrhagic fever virus strains using Virus-Like particles publication-title: PLOS Neglected Tropical Diseases doi: 10.1371/journal.pntd.0004259 – volume: 4 start-page: 417 year: 2013 ident: bib17 article-title: Factors associated with IgG positivity to Crimean-Congo hemorrhagic fever virus in the area with the highest seroprevalence in Greece publication-title: Ticks and Tick-Borne Diseases doi: 10.1016/j.ttbdis.2013.04.003 – volume: 69 start-page: 344 year: 2016 ident: bib24 article-title: The first fatal case of Crimean-Congo hemorrhagic fever caused by the AP92-Like strain of the Crimean-Congo hemorrhagic fever virus publication-title: Japanese Journal of Infectious Diseases doi: 10.7883/yoken.JJID.2015.533 – volume: 7 start-page: 1024 year: 2016 ident: bib15 article-title: Crimean-Congo hemorrhagic fever virus lineages europe 1 and europe 2 in bulgarian ticks publication-title: Ticks and Tick-Borne Diseases doi: 10.1016/j.ttbdis.2016.05.010 – volume: 80 start-page: 8834 year: 2006 ident: bib9 article-title: Crimean-Congo hemorrhagic fever virus genomics and global diversity publication-title: Journal of Virology doi: 10.1128/JVI.00752-06 – volume: 17 start-page: e1160 year: 2013 ident: bib25 article-title: Crimean-Congo hemorrhagic fever: seroprevalence and risk factors among humans in Achaia, western greece publication-title: International Journal of Infectious Diseases doi: 10.1016/j.ijid.2013.07.015 – volume: 9 year: 2009 ident: bib13 article-title: The first clinical case due to AP92 like strain of Crimean-Congo hemorrhagic fever virus and a field survey publication-title: BMC Infectious Diseases doi: 10.1186/1471-2334-9-90 – volume: 80 start-page: 533 year: 1994 ident: bib14 article-title: Establishment, maintenance and description of cell lines from the tick Ixodes scapularis publication-title: The Journal of Parasitology doi: 10.2307/3283188 – volume: 8 year: 2014 ident: bib26 article-title: Crimean-Congo hemorrhagic fever virus clades V and VI (Europe 1 and 2) in ticks in Kosovo, 2012 publication-title: PLOS Neglected Tropical Diseases doi: 10.1371/journal.pntd.0003168 – volume: 144 start-page: 93 year: 2017 ident: bib12 article-title: The role of ticks in the maintenance and transmission of Crimean-Congo hemorrhagic fever virus: a review of published field and laboratory studies publication-title: Antiviral Research doi: 10.1016/j.antiviral.2017.05.010 – volume: 64 start-page: 145 year: 2004 ident: bib31 article-title: Crimean-Congo hemorrhagic fever publication-title: Antiviral Research doi: 10.1016/j.antiviral.2004.08.001 – volume: 18 start-page: E16 year: 2012 ident: bib27 article-title: Seroepidemiological study of Crimean-Congo haemorrhagic fever in Greece, 2009-2010 publication-title: Clinical Microbiology and Infection doi: 10.1111/j.1469-0691.2011.03718.x – volume: 147 start-page: 91 year: 2017 ident: bib30 article-title: Identification of 2'-deoxy-2'-fluorocytidine as a potent inhibitor of Crimean-Congo hemorrhagic fever virus replication using a recombinant fluorescent reporter virus publication-title: Antiviral Research doi: 10.1016/j.antiviral.2017.10.008 |
| SSID | ssj0000748819 |
| Score | 2.3548925 |
| Snippet | Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| SubjectTerms | Amino Acid Substitution - genetics Amino acids Animals Arachnid Vectors - virology Cells (Biology) congo hemorrhagic fever virus Disease transmission Epidemics Europe Genetic aspects Genetic research Genetic Variation - genetics Genome, Viral - genetics Genomes Genomics Health aspects Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - pathogenicity Hemorrhagic Fever, Crimean - virology Hemorrhagic fevers Humans Infection Medical research membrane fusion Microbiology and Infectious Disease nairoviridae phylogenetic Phylogeny Tick-borne diseases tick-borne virus Ticks - virology virus pathogenesis |
| Title | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33084573 https://www.proquest.com/docview/2452977559 https://pubmed.ncbi.nlm.nih.gov/PMC7652417 https://doaj.org/article/272cbf6709bc4baf8f454ca96039430a |
| Volume | 9 |
| WOSCitedRecordID | wos000589970500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: DOA dateStart: 20130101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources (ISSN International Center) customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: M~E dateStart: 20120101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: M7P dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection (ProQuest) customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: 7X7 dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central Database Suite (ProQuest) customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: BENPR dateStart: 20120101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: PIMPY dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database (via ProQuest SciTech Premium Collection) customDbUrl: eissn: 2050-084X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000748819 issn: 2050-084X databaseCode: M2P dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3da9RAEF-0VfBF_Kyn9VilIAixl83mNvt4LS0W2uOoCufTsp-9YM3p5VLwv3dmkx4XFHzxZQPZScjszO7MZGd_Q8iBEUwzG0TCNcwm3L9NjDCjhBVaSucCz1zEmT0X02kxn8vZVqkvzAlr4YHbgTtkglkTEGXMWG50KALPudXgeGeIHB5dI_B6toKpuAYLUMxUtgfyBJjMQ39eBv8hR4eoZ4IiUv-f6_GWQeonS25Zn9NH5GHnNtJJ-7mPyR1fPSH320KSv56SnxOKMf-1p9-bdm-dlhXFkl1eV8nxsrpa0gXm1K4WGpY6GjxoML0pV01N8Vwu5nF6h8_A-7_VFM9Oliu4tqlaWF8CO2NBP4r_-utn5Mvpyefjj0lXTCGxEJSsk-A4Qvk56dPgwKthQY7NyI68keOcOe_AsIeQanD3mCngrs9EkTNjhRx56V32nOxUy8q_IFRnGk_--NQ5CM9Mqrm0Y1doxz2XOuQD8v52fJXtkMax4MW1gogDhaGiMFQUxoAcbIh_tAAbfyc7QkFtSBAVO94AXVGdrqh_6cqAvEUxK8S9qDCx5ko3da3OPl2qyZincROWDci7jigs4aut7s4pAO8IldWj3O9RwsS0ve43t9qksAuz2Sq_bGoVd7sFsAhs7bXatWEsy0YFz0U2IKKndz3O-z1VuYi44AIEyVPx8n8M1SvygOGfBbDSLN0nO-tV41-Te_ZmXdarIbkr5iK2xZDsHp1MZ5fDOAGhvWAzbAW0u7Ozi9nX352eORY |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+single+mutation+in+Crimean-Congo+hemorrhagic+fever+virus+discovered+in+ticks+impairs+infectivity+in+human+cells&rft.jtitle=eLife&rft.au=Hua%2C+Brian+L&rft.au=Scholte%2C+Florine+Em&rft.au=Ohlendorf%2C+Valerie&rft.au=Kopp%2C+Anne&rft.date=2020-10-21&rft.issn=2050-084X&rft.eissn=2050-084X&rft.volume=9&rft_id=info:doi/10.7554%2FeLife.50999&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2050-084X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2050-084X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2050-084X&client=summon |