Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communities

Terminal restriction fragment length polymorphism (T-RFLP) analysis is a popular high-throughput fingerprinting technique used to monitor changes in the structure and composition of microbial communities. This approach is widely used because it offers a compromise between the information gained and...

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Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 80; no. 3; pp. 365 - 380
Main Authors:	Schütte, Ursel M. E, Abdo, Zaid, Bent, Stephen J, Shyu, Conrad, Williams, Christopher J, Pierson, Jacob D, Forney, Larry J
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.09.2008 Springer Berlin Heidelberg Springer Springer Nature B.V
Subjects:	16S rRNA genes Archaea Archaea - genetics Automation Bacteria Bacteria - classification Bacteria - genetics Biological and medical sciences Biotechnology Change detection classification Cloning Composition DNA Fingerprinting DNA Fingerprinting - methods DNA Primers DNA Primers - genetics DNA, Ribosomal DNA, Ribosomal - genetics Environmental Microbiology Enzymes Fingerprinting Fundamental and applied biological sciences. Psychology Fungi Fungi - genetics Genes genetics Internet labor Life Sciences methods Microbial activity Microbial communities Microbial Genetics and Genomics Microbiological Techniques Microbiological Techniques - methods Microbiology Microbiomes Microorganisms Mini-Review Multivariate statistics Noise Polymerase chain reaction Polymorphism Polymorphism, Restriction Fragment Length researchers Restriction fragment length polymorphism Ribonucleic acid ribosomal RNA RNA RNA, Ribosomal, 16S RNA, Ribosomal, 16S - genetics rRNA 16S Statistical analysis Statistical methods Statistics Studies T-RFLP temporal variation Temporal variations Microbial communities Multivariate statistics T-RFLP 16S rRNA genes T-RFLP .Microbial communities Restriction fragment length polymorphism Gene Ribosomal RNA 16S-RNA Statistics Microbial community
ISSN:	0175-7598, 1432-0614, 1432-0614
Online Access:	Get full text
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