The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity

Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject’s individual age, se...

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Vydané v:Cells (Basel, Switzerland) Ročník 12; číslo 1; s. 30
Hlavní autori: Schmitz, Christoph, Alt, Christopher, Azares, Alon R., Pearce, David A., Facile, Tiffany R., Furia, John P., Maffulli, Nicola, Huang, Claire, Alt, Eckhard U.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 21.12.2022
MDPI
Predmet:
Adipose Tissue
Adipose tissues
adipose-derived regenerative cells
Age
Analysis
Antibodies
Arthritis
Body fat
Body Mass Index
Cell viability
Connective tissue
Ethnicity
Flow cytometry
Health aspects
Humans
Hypotheses
Lipectomy
Liposuction
Measurement
Methods
microfragmented fat
Minority & ethnic groups
NucleoCounter
Osteoarthritis
Point-of-Care Systems
Regenerative medicine
Sex
stem cells
United States
United States > US
Bohemia
stem cells
microfragmented fat
regenerative medicine
NucleoCounter
UA-ADRCs
adipose-derived regenerative cells
flow cytometry
ISSN:2073-4409, 2073-4409
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Popis
Shrnutí:Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject’s individual age, sex, body mass index and ethnicity. UA-ADRCs were isolated from lipoaspirate from n = 232 subjects undergoing elective lipoplasty using the Transpose RT system (InGeneron, Inc.; Houston, TX, USA). The UA-ADRCs were assessed for the number of nucleated cells, cell viability and the number of viable nucleated cells per gram of adipose tissue harvested. Cells from n = 37 subjects were further characterized using four-channel flow cytometry. The present study shows, for the first time, that key characteristics of UA-ADRCs can be independent of the subject’s age, sex, BMI and ethnicity. This result has important implications for the general applicability of UA-ADRCs in regeneration of musculoskeletal tissue. Future studies must determine whether the independence of key characteristics of UA-ADRCs of the subject’s individual age, sex, BMI and ethnicity only applies to the system used in the present study, or also to others of the more than 25 different experimental methods and commercially available systems used to isolate UA-ADRCs from lipoaspirate that have been described in the literature.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2073-4409
2073-4409
DOI:10.3390/cells12010030