Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics

Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequen...

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Vydáno v:mBio Ročník 6; číslo 6; s. e01888
Hlavní autoři: Goldberg, Brittany, Sichtig, Heike, Geyer, Chelsie, Ledeboer, Nathan, Weinstock, George M.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States American Society for Microbiology 08.12.2015
American Society of Microbiology
Témata:
Antibiotics
Antimicrobial agents
Bacteria
Clinical microbiology
Clinical trials
Communicable Diseases - diagnosis
Disease control
DNA sequencing
Drug resistance
Encephalitis
Epidemics
Genes
Genomic analysis
High-Throughput Nucleotide Sequencing - methods
High-Throughput Nucleotide Sequencing - standards
High-Throughput Nucleotide Sequencing - trends
HIV
Human immunodeficiency virus
Infectious diseases
Laboratories
Metabolism
Metagenomics
Microbiological Techniques - methods
Microbiological Techniques - standards
Microbiological Techniques - trends
Microbiomes
Minireview
Molecular Diagnostic Techniques - methods
Molecular Diagnostic Techniques - standards
Molecular Diagnostic Techniques - trends
Mutation
Next-generation sequencing
Organisms
Polymorphism
Societies, Scientific
Staphylococcus infections
Technology adoption
United States
Viruses
Whole genome sequencing
United States
ISSN:2161-2129, 2150-7511, 2150-7511
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Shrnutí:Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions.
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ISSN:2161-2129
2150-7511
2150-7511
DOI:10.1128/mBio.01888-15