Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics
Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequen...
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| Published in: | mBio Vol. 6; no. 6; p. e01888 |
|---|---|
| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Society for Microbiology
08.12.2015
American Society of Microbiology |
| Subjects: | |
| ISSN: | 2161-2129, 2150-7511, 2150-7511 |
| Online Access: | Get full text |
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| Abstract | Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions. |
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| AbstractList | Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions. ABSTRACT Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions. Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions.Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions. |
| Author | Geyer, Chelsie Weinstock, George M. Goldberg, Brittany Sichtig, Heike Ledeboer, Nathan |
| Author_xml | – sequence: 1 givenname: Brittany surname: Goldberg fullname: Goldberg, Brittany organization: Division of Pediatric Infectious Diseases, Children's National Medical Center, Washington, DC, USA – sequence: 2 givenname: Heike surname: Sichtig fullname: Sichtig, Heike organization: Division of Microbiology Devices, Food and Drug Administration, Office of In Vitro Diagnostics and Radiological Health, Silver Spring, Maryland, USA – sequence: 3 givenname: Chelsie surname: Geyer fullname: Geyer, Chelsie organization: American Society for Microbiology, Washington, DC, USA – sequence: 4 givenname: Nathan surname: Ledeboer fullname: Ledeboer, Nathan organization: Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA – sequence: 5 givenname: George M. surname: Weinstock fullname: Weinstock, George M. organization: The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26646014$$D View this record in MEDLINE/PubMed |
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| Snippet | Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for... ABSTRACT Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new... |
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| SubjectTerms | Antibiotics Antimicrobial agents Bacteria Clinical microbiology Clinical trials Communicable Diseases - diagnosis Disease control DNA sequencing Drug resistance Encephalitis Epidemics Genes Genomic analysis High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards High-Throughput Nucleotide Sequencing - trends HIV Human immunodeficiency virus Infectious diseases Laboratories Metabolism Metagenomics Microbiological Techniques - methods Microbiological Techniques - standards Microbiological Techniques - trends Microbiomes Minireview Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards Molecular Diagnostic Techniques - trends Mutation Next-generation sequencing Organisms Polymorphism Societies, Scientific Staphylococcus infections Technology adoption United States Viruses Whole genome sequencing |
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| Title | Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics |
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