The yeast Red1 protein localizes to the cores of meiotic chromosomes

Mutants in the meiosis-specific RED1 gene of S. cerevisiae fail to make any synaptonemal complex (SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein, Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:The Journal of cell biology Ročník 136; číslo 5; s. 957
Hlavní autoři: Smith, A V, Roeder, G S
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 10.03.1997
Témata:
Cell Nucleolus - chemistry
Chromosomes, Fungal - chemistry
DNA-Binding Proteins - analysis
Fungal Proteins - analysis
Fungal Proteins - genetics
Meiosis
Mutation
Nuclear Proteins
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins
Spores, Fungal
Synaptonemal Complex
ISSN:0021-9525
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Mutants in the meiosis-specific RED1 gene of S. cerevisiae fail to make any synaptonemal complex (SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein, Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed axial elements present in a zip1 mutant, suggesting that Red1 is a component of the lateral elements of mature SCs. Anti-Red1 staining is confined to the cores of meiotic chromosomes and is not associated with the loops of chromatin that lie outside the SC. Analysis of the spo11 mutant demonstrates that Red1 localization does not depend upon meiotic recombination. The localization of Red1 has been compared with two other meiosis-specific components of chromosomes, Hop1 and Zip1; Zip1 serves as a marker for synapsed chromosomes. Double labeling of wild-type meiotic chromosomes with anti-Zip1 and anti-Red1 antibodies demonstrates that Red1 localizes to chromosomes both before and during pachytene. Double labeling with anti-Hop1 and anti-Red1 antibodies reveals that Hop1 protein localizes only in areas that also contain Red1, and studies of Hop1 localization in a red1 null mutant demonstrate that Hop1 localization depends on Red1 function. These observations are consistent with previous genetic studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene chromosomes or in synapsed chromosomal regions.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9525
DOI:10.1083/jcb.136.5.957