Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation: Distribution of the genes in BTEX degrading isolates and in subsurface soils of a BTEX contaminated industrial site

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allo...

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 64; no. 2; pp. 250 - 265
Main Authors:	Hendrickx, Barbara, Junca, Howard, Vosahlova, Jolana, Lindner, Antje, Rüegg, Irene, Bucheli-Witschel, Margarete, Faber, Folkert, Egli, Thomas, Mau, Margit, Schlömann, Michael, Brennerova, Maria, Brenner, VLadimir, Pieper, Dietmar H., Top, Eva M., Dejonghe, Winnie, Bastiaens, Leen, Springael, Dirk
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier B.V 01.02.2006 Elsevier Science
Subjects:	Actinobacteria Actinobacteria - genetics Actinobacteria - metabolism Aerobic BTEX biodegradation Bacteria, Aerobic - genetics Bacteria, Aerobic - metabolism Bacterial Proteins Bacteriology Biodegradation, Environmental Biological and medical sciences BTEX contaminated site BTEX degrading isolates Carbohydrate Epimerases Catabolic gene distribution DNA Primers Environmental Pollution Fundamental and applied biological sciences. Psychology Gene Transfer, Horizontal Genes, Bacterial - physiology Genetics Hydrocarbons - metabolism Industrial Waste Microbiology PCR detection Polymerase Chain Reaction - methods Proteobacteria Proteobacteria - genetics Proteobacteria - metabolism Pseudomonas Soil Microbiology Soil Pollutants - metabolism Species Specificity Substrate Specificity PCR detection Aerobic BTEX biodegradation BTEX contaminated site Catabolic gene distribution BTEX degrading isolates Pseudomonadales Biodegradation Eubacteria Toluene Pseudomonas Polymerase chain reaction Gene Benzene Xylene Bacteria Pseudomonadaceae Actinobacteria Catabolism Detection
ISSN:	0167-7012, 1872-8359
Online Access:	Get full text
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