M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and microorganisms

Hypervariable polymorphic patterns were detected with M13 phage DNA as a probe in genomic DNA of organisms belonging to different taxonomic groups including animals (vertebrates and invertebrates), plants and microorganisms. Individual-specific restriction pattern analysis (DNA fingerprinting) with...

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Veröffentlicht in:FEBS letters Jg. 233; H. 2; S. 388
Hauptverfasser: Ryskov, A P, Jincharadze, A G, Prosnyak, M I, Ivanov, P L, Limborska, S A
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 20.06.1988
Schlagworte:
Animals
Coliphages - genetics
DNA - genetics
DNA - isolation & purification
DNA Restriction Enzymes
DNA, Viral - genetics
Escherichia coli - genetics
Female
Humans
Male
Paternity
Pedigree
Plants - genetics
Polymorphism, Genetic
Species Specificity
ISSN:0014-5793
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Zusammenfassung:Hypervariable polymorphic patterns were detected with M13 phage DNA as a probe in genomic DNA of organisms belonging to different taxonomic groups including animals (vertebrates and invertebrates), plants and microorganisms. Individual-specific restriction pattern analysis (DNA fingerprinting) with this probe proved to be useful for individual identification, analysis of somatic stability and paternity testing in man. The nuclear type of inheritance indicates that the hypervariable DNA regions in question are located in the chromosomes, not in the mitochondrial DNA. The data obtained also demonstrate a potential range of M13 DNA applications as a probe for DNA fingerprinting of animals, plants and microorganisms, particularly for the determination of inbred lines, identification of bacterial strains and establishing stock, variety and strain distinctions.
Bibliographie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0014-5793
DOI:10.1016/0014-5793(88)80467-8