Cells respond to mechanical stress by rapid disassembly of caveolae

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretchin...

Full description

Saved in:
Bibliographic Details
Published in:Cell Vol. 144; no. 3; p. 402
Main Authors: Sinha, Bidisha, Köster, Darius, Ruez, Richard, Gonnord, Pauline, Bastiani, Michele, Abankwa, Daniel, Stan, Radu V, Butler-Browne, Gillian, Vedie, Benoit, Johannes, Ludger, Morone, Nobuhiro, Parton, Robert G, Raposo, Graça, Sens, Pierre, Lamaze, Christophe, Nassoy, Pierre
Format: Journal Article
Language:English
Published: United States 04.02.2011
Subjects:
Actins - physiology
Adenosine Triphosphate - physiology
Animals
Caveolae - physiology
Caveolae - ultrastructure
Cell Line
Endothelial Cells - cytology
Endothelial Cells - physiology
Humans
Mice
Muscle Cells - cytology
Muscle Cells - physiology
Stress, Mechanical
ISSN:1097-4172, 1097-4172
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin- and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin- and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1097-4172
1097-4172
DOI:10.1016/j.cell.2010.12.031