Integrated analysis of the expression profiles of the lncRNA-miRNA-mRNA ceRNA network in CASMCs under hypoxia and normoxia conditions in yak heart

Hypoxia causes the occurrence of right heart hypertrophy and right heart failure. However, the yak living in the hypoxic environment, does not exhibit hypoxia-related pathological features. Therefore, It is of great significance to explore the hypoxia adaptation mechanism of yak heart. In this study...

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Bibliographic Details
Published in:Scientific reports Vol. 15; no. 1; pp. 9165 - 15
Main Authors: Yang, Shanshan, Cui, Yan, Yu, Sijiu, He, Junfeng, Ma, Rui, Bai, Xuefeng, Zhang, Hui, Zhao, Pengfei
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 17.03.2025
Nature Publishing Group
Nature Portfolio
Subjects:
1-Phosphatidylinositol 3-kinase
631/181
631/208
631/337
631/443
631/80
Adaptation
AKT protein
Animals
Cattle
Cell Hypoxia
CeRNA network
Congestive heart failure
Coronary vascular smooth muscle cells
Coronary Vessels - cytology
Coronary Vessels - metabolism
Extracellular matrix
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Heart
Humanities and Social Sciences
Hypertrophy
Hypoxia
Hypoxia - genetics
Hypoxic adaptation
Immune system
LncRNA
MAP kinase
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
MiRNA
Molecular modelling
multidisciplinary
Myocardium - metabolism
Myocytes, Smooth Muscle - metabolism
Non-coding RNA
Regulatory sequences
RNA, Competitive Endogenous
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Science
Science (multidisciplinary)
Smooth muscle
TOR protein
Transcription factors
Transcriptome
Transforming growth factor-b
Yak
LncRNA
CeRNA network
Yak
Hypoxic adaptation
Coronary vascular smooth muscle cells
MiRNA
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Hypoxia causes the occurrence of right heart hypertrophy and right heart failure. However, the yak living in the hypoxic environment, does not exhibit hypoxia-related pathological features. Therefore, It is of great significance to explore the hypoxia adaptation mechanism of yak heart. In this study, the yak heart coronary vascular smooth muscle cells (CASMCs) were treated with 21% O 2 (normoxic group) and 5% O 2 (hypoxic group). The results showed that hypoxia could promote the proliferation of CASMCs. Subsequently, we sequenced CASMCs in normoxic and hypoxic groups. The analysis revealed differential expression of 835 mRNAs, 285 lncRNAs and 126 miRNAs were between the two groups. GO and KEGG analysis showed that the differentially expressed genes were predominantly associated with extracellular matrix components, transcription factor activity, protein binding, immune system processes, metabolic processes and cell development processes and TGF-β, MAPK, cAMP, mTOR, PI3K-Akt and other signaling pathways. By constructing a network of mRNAs, miRNAs and lncRNAs based on the major differentially expressed RNAs, core regulatory elements associated with hypoxic adaptive function were identified. Our study may help to prove the potential role of differential genes related to hypoxic adaptation, and enhanced understanding of the molecular mechanisms of hypoxic adaptation in yak heart.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-025-85483-4