Comparative analysis of organoid, air-liquid interface, and direct infection models for studying pathogen–host interactions in endometrial tissue

The endometrium is a critical component of female reproductive health. Endometritis can significantly affect women’s health, leading to complications such as infertility, pregnancy failure, and intrauterine adhesions. Therefore, establishing a reliable and effective model of endometritis is essentia...

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Vydané v:	Scientific reports Ročník 15; číslo 1; s. 8531 - 11
Hlavní autori:	Zhang, Xin, Fan, Linyuan, Zhang, Li, Liu, Zhaohui
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 12.03.2025 Nature Publishing Group Nature Portfolio
Predmet:	631/1647/2204 631/1647/767 692/699/255 Air-liquid interface Animals Cell culture Comparative analysis E coli Endometrial organoids Endometritis Endometritis - microbiology Endometritis - pathology Endometrium Endometrium - microbiology Endometrium - pathology Escherichia coli - pathogenicity Escherichia coli Infections - microbiology Escherichia coli Infections - pathology Female Females Host-Pathogen Interactions Humanities and Social Sciences Humans Immune response Infection model Infections Infertility Microinjection Microinjections Models, Biological multidisciplinary Organoids Organoids - microbiology Pregnancy complications Replication Reproductive health Science Science (multidisciplinary) Suspension culture Transwell Endometrial organoids Air-liquid interface Infection model Transwell
ISSN:	2045-2322, 2045-2322
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Popis
Shrnutí:	The endometrium is a critical component of female reproductive health. Endometritis can significantly affect women’s health, leading to complications such as infertility, pregnancy failure, and intrauterine adhesions. Therefore, establishing a reliable and effective model of endometritis is essential for advancing research in female reproductive health. The air-liquid interface culture models epithelial exposure to both air and medium, supporting the study of apical-basal polarity and immune responses, but lacks the three-dimensional structure of organoids. Microinjection delivers bacteria directly into the organoid lumen, facilitating the study of bacterial invasion and replication, though it requires advanced technical skills and may not fully replicate natural infections. The direct infection of organoids in suspension culture offers a more realistic model of ascending infections by exposing the basal epithelial surfaces to bacteria, more closely mimicking in vivo conditions. Among the models tested, the direct infection method most accurately mirrors the progression of E. coli infection, showing its advantage in studying bacterial adhesion, replication, and epithelial barrier disruption. Our comparative analysis of microinjection, direct infection, and the ALI model highlighted distinct advantages and challenges associated with each. Microinjection offers precise delivery but is hindered by technical complexity and equipment demands. The ALI model, despite its efficacy, requires extended culture times and limits direct visualization of cell development. Conversely, the direct infection model, which involves the simple removal of Matrigel, proves to be user-friendly, cost-effective, and permits continuous observation of cell behavior. Nonetheless, the direct infection model still presents certain limitations that warrant further optimization.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-025-93374-x