High‐viscosity sample‐injection device for serial femtosecond crystallography at atmospheric pressure

A sample‐injection device has been developed at SPring‐8 Angstrom Compact Free‐Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas fl...

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Veröffentlicht in:Journal of applied crystallography Jg. 52; H. 6; S. 1280 - 1288
Hauptverfasser: Shimazu, Yoshiaki, Tono, Kensuke, Tanaka, Tomoyuki, Yamanaka, Yasuaki, Nakane, Takanori, Mori, Chihiro, Terakado Kimura, Kanako, Fujiwara, Takaaki, Sugahara, Michihiro, Tanaka, Rie, Doak, R. Bruce, Shimamura, Tatsuro, Iwata, So, Nango, Eriko, Yabashi, Makina
Format: Journal Article
Sprache:Englisch
Veröffentlicht: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.12.2019
Blackwell Publishing Ltd
Schlagworte:
Atmospheric pressure
Cartridges
Crystallography
Data collection
Dynamic structural analysis
Gas flow
Grease
Injection
Lysozyme
microcrystal injection
Microcrystals
Nozzles
Research Papers
Reservoirs
room‐temperature crystallography
serial femtosecond crystallography
Suction
Viscosity
Water flow
X‐ray free‐electron lasers
microcrystal injection
room-temperature crystallography
X-ray free-electron lasers
serial femtosecond crystallography
ISSN:1600-5767, 0021-8898, 1600-5767
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Zusammenfassung:A sample‐injection device has been developed at SPring‐8 Angstrom Compact Free‐Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge‐type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature‐regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A2A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg‐white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump–probe techniques. A high‐viscosity cartridge‐type injector for serial femtosecond crystallography has been developed at SPring‐8 Angstrom Compact Free‐Electron Laser.
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SourceType-Scholarly Journals-1
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Present address: Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, England.
ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S1600576719012846