Silent spread of mcr-9 in ESBL-producing Enterobacteriaceae clinical isolates, Jimma, Ethiopia

Mobile colistin resistance (MCR) genes, have been increasingly identified worldwide, but their presence and characteristics in Africa remain poorly understood. Herein, we characterized a silent mcr-9 gene carried in Enterobacteriaceae from Ethiopia. In a study investigating genomic epidemiology of E...

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Vydáno v:PloS one Ročník 20; číslo 11; s. e0336440
Hlavní autoři: Sewunet, Tsegaye, Razavi, Mohammad, Tellapragada, Chaitanya, Giske, Christian G.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Public Library of Science 18.11.2025
Public Library of Science (PLoS)
Témata:
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics
Antimicrobial agents
Bacterial infections
Bacterial Proteins - genetics
Beta lactamases
beta-Lactamases - genetics
beta-Lactamases - metabolism
Chronic illnesses
Clinical isolates
Colistin
Colistin - pharmacology
Drug resistance
Drug Resistance, Bacterial - genetics
E coli
Enterobacter cloacae
Enterobacteriaceae
Enterobacteriaceae - drug effects
Enterobacteriaceae - enzymology
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Enterobacteriaceae Infections - drug therapy
Enterobacteriaceae Infections - epidemiology
Enterobacteriaceae Infections - microbiology
Epidemiology
Ethiopia - epidemiology
Feces
Gene sequencing
Genes
Genetics
Genomes
Genomics
Gram-negative bacilli
Humans
Klebsiella
Microbial Sensitivity Tests
Nosocomial infections
Plasmids
Plasmids - genetics
Salmonella
Secretions
Transposons
Virulence
Whole Genome Sequencing
Ethiopia
South Africa
ISSN:1932-6203, 1932-6203
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Shrnutí:Mobile colistin resistance (MCR) genes, have been increasingly identified worldwide, but their presence and characteristics in Africa remain poorly understood. Herein, we characterized a silent mcr-9 gene carried in Enterobacteriaceae from Ethiopia. In a study investigating genomic epidemiology of ESBL-producing Gram-negative bacilli from clinical samples in 2016, eleven isolates were found to encode mcr-9 genes. Whole genome sequencing, combining Illumina and MinION Nanopore technologies were performed for in-depth investigation of the genome and understand the genetic contexts of mcr-9 and its associated plasmids. The mcr-9 genes were detected in Enterobacter cloacae (n = 8), Escherichia coli (n = 1), Klebsiella pneumoniae (n = 1), and K. michiganensis (n = 1). They were isolated from urine (54.5%, 6/11), wound secretions (27.3%, 3/11), and fecal samples (n = 18.2%, 2/11). In addition to mcr-9, all isolates encoded blaCTX-M-15, and aac (6')-Ib-cr among several other resistance genes. These isolates were susceptible to colistin (MIC ≤ 0.5 mg/L). The E. cloacae strains belong to three different sequence types (ST): ST114 (n = 3), ST184 (n = 3), ST254 (n = 1), and Enterobacter mori ST2197 (n = 1)), whereas the E. coli and the K. pneumoniae strains belonged to ST410 and ST337. IncHI2 and IncHI2A plasmid replicons were present in all isolates. Although the genetic content of plasmids carrying the mcr-9 genes varied, the genetic contexts surrounding all mcr genes within ±10kb region were largely consistent, mostly flanked by composite transposons such as IS26, and IS903B. A silent mcr-9 gene was detected among ESBL-producing Enterobacteriaceae isolates. The IncHI2 plasmids encoding the mcr-9 had 25% genetic content dissimilarity.
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ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0336440