Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes

Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph...

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Vydáno v:	eLife Ročník 11
Hlavní autoři:	Yang, Peng, Chen, Yaohui, Huang, Zhiqi, Xia, Huidan, Cheng, Ling, Wu, Hao, Zhang, Yueling, Wang, Fan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England eLife Sciences Publications Ltd 06.10.2022 eLife Sciences Publications, Ltd
Témata:	Animals Arthropod Proteins - genetics differentiation Gene expression Genomics Granulocytes Hemocytes Hemolymph Immune response Immunity, Innate - genetics Immunology and Inflammation Infectious diseases Innate immunity invertebrate Invertebrates Leukocytes (granulocytic) Macrophages Macrophages - metabolism Mammals - genetics Monocytes myeloid cell evolution NLR Family, Pyrin Domain-Containing 3 Protein - metabolism Pathogens Penaeidae - genetics Penaeus vannamei Phagocytes Phagocytes - metabolism Proteins Sequence analysis Sequence Analysis, RNA Vertebrates macrophages differentiation inflammation Penaeus vannamei myeloid cell evolution invertebrate immunology
ISSN:	2050-084X, 2050-084X
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph. Our results identified a novel macrophage-like subset called monocytic hemocytes 2 (MH2) defined by the expression of certain marker genes, including Nlrp3 and Casp1 . This subtype of shrimp hemocytes is phagocytic and expresses markers that indicate some conservation with mammalian macrophages. Combined, our work resolves the heterogenicity of hemocytes in a very economically important aquatic species and identifies a novel innate immune cell subset that is likely a critical player in the immune responses of shrimp to threatening infectious diseases affecting this industry.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2050-084X 2050-084X
DOI:	10.7554/eLife.80127