Biological study of skin wound treated with Alginate/Carboxymethyl cellulose/chorion membrane, diopside nanoparticles, and Botox A

A hydrogel-based wound dressing with desirable properties is necessary for achieving functional skin integrity post-injury. This study focuses on preparing a hydrogel using Alginate/Carboxymethyl cellulose (Alg/CMC) as a base material. To evaluate its regenerative effects on full-thickness wounds, d...

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Bibliographic Details
Published in:npj Regenerative medicine Vol. 9; no. 1; pp. 9 - 20
Main Authors: Mahheidari, Naimeh, Kamalabadi-Farahani, Mohammad, Nourani, Mohammad Reza, Atashi, Amir, Alizadeh, Morteza, Aldaghi, Niloofar, Salehi, Majid
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 27.02.2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/61/490
692/308/1426
Biomaterials
Biomedical and Life Sciences
Biomedicine
Blood vessels
Botulinum toxin
Cell adhesion & migration
Cell Biology
Cellulose
Connective tissue
Extracellular matrix
Growth factors
Hydrogels
Immunology
Morphology
Nanoparticles
Regenerative medicine
Regenerative Medicine/Tissue Engineering
Skin integrity
Spectrum analysis
Stem Cells
Tissue engineering
Wound healing
ISSN:2057-3995, 2057-3995
Online Access:Get full text
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Summary:A hydrogel-based wound dressing with desirable properties is necessary for achieving functional skin integrity post-injury. This study focuses on preparing a hydrogel using Alginate/Carboxymethyl cellulose (Alg/CMC) as a base material. To evaluate its regenerative effects on full-thickness wounds, diopside nanoparticles and Botulinum toxin A (BTX-A) were incorporated into the hydrogel along with chorion membrane. The diopside nanoparticles (DNPs) act as a proangiogenic factor, promoting proliferation and regulating inflammation, while the chorion membrane facilitates these processes. Additionally, BTX-A prevents scar formation and aids in wound closure. The nanoparticles and hydrogel were characterized using various techniques, and their cytocompatibility was assessed. In vivo studies and quantitative polymerase chain reaction analysis showed that wound area reduction was significant after two weeks of treatment with the Alg/CMC/ChNPs/DNPs/BTX-A hydrogel. Overall, this scaffold demonstrated potential for promoting tissue regeneration and new epithelization formation, making it a promising candidate for enhancing skin restoration in wound treatments.
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ISSN:2057-3995
2057-3995
DOI:10.1038/s41536-024-00354-2