A single-dose intranasal live-attenuated codon deoptimized vaccine provides broad protection against SARS-CoV-2 and its variants

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) continues its significant health and economic impact globally. Despite the success of spike-protein vaccines in preventing severe disease, long-lasting protection against emerging variants and the prevention of breakthrough infec...

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Vydané v:Nature communications Ročník 15; číslo 1; s. 7225 - 19
Hlavní autori: Liu, Xiang, Ng, Wern Hann, Zusinaite, Eva, Freitas, Joseph, Taylor, Adam, Yerragunta, Venugopal, Aavula, Shukra Madhaha, Gorriparthi, Sambaiah, Ponsekaran, Santhakumar, Bonda, Rama Lakshmi, Mani, Priyanka, Nimmagadda, Sridevi V., Wang, Sainan, Lello, Laura Sandra, Zaid, Ali, Dua, Ujjwal, Taft-Benz, Sharon A., Anderson, Elizabeth, Baxter, Victoria K., Sarkar, Sanjay, Ling, Zheng L., Ashhurst, Thomas M., Cheng, Samuel M. S., Pattnaik, Priyabrata, Kanakasapapathy, Anand Kumar, Baric, Ralph S., Burt, Felicity J., Peiris, Malik, Heise, Mark T., King, Nicholas J. C., Merits, Andres, Lingala, Rajendra, Mahalingam, Suresh
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 26.08.2024
Nature Publishing Group
Nature Portfolio
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Administration, Intranasal
Animal models
Animals
Antibodies
Antibodies, Neutralizing - immunology
Antibodies, Viral - immunology
Attenuation
Chlorocebus aethiops
Codon
Coronaviruses
COVID-19
COVID-19 - immunology
COVID-19 - prevention & control
COVID-19 - virology
COVID-19 Vaccines - administration & dosage
COVID-19 Vaccines - immunology
Cricetinae
Disease transmission
Economic impact
Female
Hamsters
Humanities and Social Sciences
Humans
Immunity
Immunization
Lymphocytes T
Male
Mice
multidisciplinary
Proteins
SARS-CoV-2 - genetics
SARS-CoV-2 - immunology
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Spike Glycoprotein, Coronavirus - genetics
Spike Glycoprotein, Coronavirus - immunology
T-Lymphocytes - immunology
Vaccines
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - genetics
Vaccines, Attenuated - immunology
Viral diseases
ISSN:2041-1723, 2041-1723
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Shrnutí:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) continues its significant health and economic impact globally. Despite the success of spike-protein vaccines in preventing severe disease, long-lasting protection against emerging variants and the prevention of breakthrough infections and transmission remain elusive. We generate an intranasal live-attenuated SARS-CoV-2 vaccine, CDO-7N-1, using codon deoptimization. CDO-7N-1 shows highly attenuated replication and minimal or no lung pathology in vivo over multiple passages. It induces robust mucosal and systemic neutralizing antibody and T-cell subset responses, in mice (female K18-hACE2 and male HFH4-hACE2 mice), hamsters, and macaques triggered by a single immunization. Mice and hamsters vaccinated with CDO-7N-1 are protected from challenge with wild-type (WT) SARS-CoV-2 and other variants of concern. Serum from vaccinated animals neutralizes WT SARS-CoV-2, variants of concern (beta and delta), variants of interest (omicron XBB.1.5) and SARS-CoV-1. Antibody responses are sustained and enhanced by repeated immunization or infection with WT SARS-CoV-2. Immunity against all SARS-CoV-2 proteins by CDO-7N-1 should improve efficacy against future SARS-CoV-2 variants. Current SARS-CoV-2 vaccines require several injections for optimal immune protection. Here, the authors report an intranasal live-attenuated vaccine that induces long-term immunity following a single dose and protects from SARS-CoV-2 wildtype and variants in non-human primate and small animal models.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-024-51535-y