Comparison of infection and human immune responses of two SARS-CoV-2 strains in a humanized hACE2 NIKO mouse model

The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized hACE2 (adenovirus expressing hACE2) NOD-SCID IL2R...

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Veröffentlicht in:Scientific reports Jg. 13; H. 1; S. 12484 - 10
Hauptverfasser: Yong, Kylie Su Mei, Anderson, Danielle E., Zheng, Adrian Kang Eng, Liu, Min, Tan, Sue Yee, Tan, Wilson Wei Sheng, Chen, Qingfeng, Wang, Lin-Fa
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 01.08.2023
Nature Publishing Group
Nature Portfolio
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Adenoviruses
Animal models
Animals
Antigens
Chemokines
COVID-19
Cytokines
Cytomegalovirus
Disease
Disease Models, Animal
Experimental infection
Experiments
Global economy
Histology
Humanities and Social Sciences
Humans
Immune response
Immune system
Immunology
Infections
Inflammation
Lung
Lung cancer
Mice
Mice, Inbred NOD
Mice, SCID
Mice, Transgenic
multidisciplinary
Mutants
Pandemics
Pathogenesis
Plasmids
Proteins
SARS-CoV-2
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Spleen
Stem cells
Therapeutic applications
ISSN:2045-2322, 2045-2322
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Zusammenfassung:The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized hACE2 (adenovirus expressing hACE2) NOD-SCID IL2Rγ −/− (NIKO) mouse model and compare infection with ancestral and mutant (SARS-CoV-2-∆382) strains of SARS-CoV-2. Immune cell infiltration, inflammation, lung damage and pro-inflammatory cytokines and chemokines was observed in humanized hACE2 NIKO mice. Humanized hACE2 NIKO mice infected with the ancestral and mutant SARS-CoV-2 strain had lung inflammation and production of pro-inflammatory cytokines and chemokines. This model can aid in examining the pathological basis of SARS-CoV-2 infection in a human immune environment and evaluation of therapeutic interventions.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-39628-y