DNA methylation patterns in umbilical cord blood from infants of methadone maintained opioid dependent mothers

Methadone maintenance treatment for opioid dependent mothers is standard of care. Infants of methadone maintained opioid dependent (MMOD) mothers have better outcomes compared to infants of opioid dependent mothers without treatment. However, when compared to non-exposed infants, infants of MMOD mot...

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Bibliographic Details
Published in:Scientific reports Vol. 14; no. 1; pp. 17298 - 12
Main Authors: Adegboyega, Oluwatobi, Gayen nee’ Betal, Suhita, Urday, Pedro, Huang, Rachel, Bodycot, Katherine, Al-Kouatly, Huda B., Solarin, Kolawole, Chan, Joanna S. Y., Addya, Sankar, Boelig, Rupsa C., Aghai, Zubair H.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 27.07.2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/208/176
692/700/1720/3187
Adult
Analgesics, Opioid - therapeutic use
Chorioamnionitis
Cord blood
Deoxyribonucleic acid
DNA
DNA Methylation
Female
Fetal Blood - metabolism
Humanities and Social Sciences
Humans
Infant, Newborn
Infants
Male
Methadone
Methadone - therapeutic use
Mothers
multidisciplinary
Narcotics
Neonates
Opiate Substitution Treatment
Opioid-Related Disorders - genetics
Opioids
Pilot Projects
Pregnancy
Pregnancy Complications - drug therapy
Pregnancy Complications - genetics
Science
Science (multidisciplinary)
Umbilical cord
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Methadone maintenance treatment for opioid dependent mothers is standard of care. Infants of methadone maintained opioid dependent (MMOD) mothers have better outcomes compared to infants of opioid dependent mothers without treatment. However, when compared to non-exposed infants, infants of MMOD mothers are associated with worse outcomes. We conducted a pilot study to examine genome wide differential DNA methylation using cord blood samples from sixteen term and near-term infants of MMOD and opioid naïve mothers, excluding Infants with chorioamnionitis. A total of 152 differentially methylated loci were identified at a difference >  + 2, < − 2 and p-value < 0.05. There were 90 hypermethylated loci (59 annotated genes) and 62 hypomethylated loci (38 annotated genes) observed. The hypermethylated and hypomethylated DNA changes involved multiple genes, pathways and networks that may explain some of the changes seen in infants of MMOD mothers. Top hypermethylated and hypomethylated genes involved areas of cell growth, neurodevelopment, vision and xenobiotic metabolism functions. Our data may explain the role of key pathways and genes relevant to neonatal outcomes seen from methadone exposure in pregnancy. Functional studies on the identified pathways and genes could lead to improved understanding of the mechanisms and identify areas for intervention.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-66899-w