Accurate determination of CRISPR-mediated gene fitness in transplantable tumours

Assessing tumour gene fitness in physiologically-relevant model systems is challenging due to biological features of in vivo tumour regeneration, including extreme variations in single cell lineage progeny. Here we develop a reproducible, quantitative approach to pooled genetic perturbation in patie...

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Bibliographic Details
Published in:	Nature communications Vol. 13; no. 1; pp. 4534 - 19
Main Authors:	Eirew, Peter, O’Flanagan, Ciara, Ting, Jerome, Salehi, Sohrab, Brimhall, Jazmine, Wang, Beixi, Biele, Justina, Algara, Teresa, Lee, So Ra, Hoang, Corey, Yap, Damian, McKinney, Steven, Bates, Cherie, Kong, Esther, Lai, Daniel, Beatty, Sean, Andronescu, Mirela, Zaikova, Elena, Funnell, Tyler, Ceglia, Nicholas, Chia, Stephen, Gelmon, Karen, Mar, Colin, Shah, Sohrab, Roth, Andrew, Bouchard-Côté, Alexandre, Aparicio, Samuel
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 04.08.2022 Nature Publishing Group Nature Portfolio
Subjects:	13/44 42/109 42/47 45/23 49/109 631/154/556 631/67/1347 631/67/2329 631/67/69 64/60 Animals Bayes Theorem Bayesian analysis Breast cancer Breast Neoplasms - genetics Cell lineage Clustered Regularly Interspaced Short Palindromic Repeats CRISPR Data analysis Disease Models, Animal Female Fitness Heterografts Humanities and Social Sciences Humans In vivo methods and tests Mathematical models MDM2 protein multidisciplinary Outliers (statistics) p53 Protein Perturbation Progeny Reproductive fitness Science Science (multidisciplinary) Transplants Transplants & implants Tumors Uncertainty Xenograft Model Antitumor Assays Xenografts Xenotransplantation
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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