Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

Agrobacterium -mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a s...

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Bibliographic Details
Published in:Nature communications Vol. 13; no. 1; pp. 2581 - 14
Main Authors: Raman, Vidhyavathi, Rojas, Clemencia M., Vasudevan, Balaji, Dunning, Kevin, Kolape, Jaydeep, Oh, Sunhee, Yun, Jianfei, Yang, Lishan, Li, Guangming, Pant, Bikram D., Jiang, Qingzhen, Mysore, Kirankumar S.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 11.05.2022
Nature Publishing Group
Nature Portfolio
Subjects:
45/22
45/88
631/449/2169/2673
631/449/447/2311
631/61/447/8
96/106
96/44
Agricultural biotechnology
Agricultural practices
Agricultural research
Agrobacterium
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - metabolism
Alfalfa
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biotechnology
Defense mechanisms
Effector cells
Effectors
Flowers & plants
Genetic transformation
Histone H2A
Host plants
Humanities and Social Sciences
multidisciplinary
Plant bacterial diseases
Plant cells
Plant Cells - metabolism
Plant Diseases - microbiology
Plant growth
Plant species
Proteins
Pseudomonas
Pseudomonas syringae - genetics
Pseudomonas syringae - metabolism
Science
Science (multidisciplinary)
Secretion
Species
Transformations
Type III Secretion Systems - genetics
Type III Secretion Systems - metabolism
ISSN:2041-1723, 2041-1723
Online Access:Get full text
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Summary:Agrobacterium -mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a strategy to increase AMT by engineering Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and individually deliver the P. syringae effectors AvrPto, AvrPtoB, or HopAO1 to suppress host defense responses. Using the engineered Agrobacterium , we demonstrate increase in AMT of wheat, alfalfa and switchgrass by ~250%–400%. We also show that engineered A. tumefaciens expressing a T3SS can deliver a plant protein, histone H2A-1, to enhance AMT. This strategy is of great significance to both basic research and agricultural biotechnology for transient and stable transformation of recalcitrant plant species/varieties and to deliver proteins into plant cells in a non-transgenic manner. Agrobacterium infection can cause defense responses in many plants, which leads to transformation recalcitrance. Here, the authors express type III secretion system in Agrobacterium to deliver effector proteins into plant cells to suppress host defense responses and thus enhance transformation in some plant species.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-022-30180-3