Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs

Most cells constitutively secrete mitochondrial DNA and proteins in extracellular vesicles (EVs). While EVs are small vesicles that transfer material between cells, Mitochondria-Derived Vesicles (MDVs) carry material specifically between mitochondria and other organelles. Mitochondrial content can e...

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Vydané v:Nature communications Ročník 12; číslo 1; s. 1971 - 12
Hlavní autori: Todkar, Kiran, Chikhi, Lilia, Desjardins, Véronique, El-Mortada, Firas, Pépin, Geneviève, Germain, Marc
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 30.03.2021
Nature Publishing Group
Nature Portfolio
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14/19
38
38/77
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631/80/313
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Alarmins - metabolism
Atrophy
Damage patterns
DNA, Mitochondrial - metabolism
Extracellular vesicles
Extracellular Vesicles - metabolism
GTP Phosphohydrolases - metabolism
Humanities and Social Sciences
Humans
Immune response
Inflammation
Inflammation - metabolism
Lysosomes
Lysosomes - metabolism
Mitochondria
Mitochondria - metabolism
Mitochondrial DNA
Mitochondrial Proteins - metabolism
Movement disorders
multidisciplinary
Neurodegenerative diseases
Nexin
Optic atrophy
Organelles
Packaging
Parkin protein
Parkinson Disease - metabolism
Parkinson's disease
Proteins
Quality control
Science
Science (multidisciplinary)
Sorting Nexins - metabolism
Ubiquitin-Protein Ligases - metabolism
Vesicles
ISSN:2041-1723, 2041-1723
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Popis
Shrnutí:Most cells constitutively secrete mitochondrial DNA and proteins in extracellular vesicles (EVs). While EVs are small vesicles that transfer material between cells, Mitochondria-Derived Vesicles (MDVs) carry material specifically between mitochondria and other organelles. Mitochondrial content can enhance inflammation under pro-inflammatory conditions, though its role in the absence of inflammation remains elusive. Here, we demonstrate that cells actively prevent the packaging of pro-inflammatory, oxidized mitochondrial proteins that would act as damage-associated molecular patterns (DAMPs) into EVs. Importantly, we find that the distinction between material to be included into EVs and damaged mitochondrial content to be excluded is dependent on selective targeting to one of two distinct MDV pathways. We show that Optic Atrophy 1 (OPA1) and sorting nexin 9 (Snx9)-dependent MDVs are required to target mitochondrial proteins to EVs, while the Parkinson’s disease-related protein Parkin blocks this process by directing damaged mitochondrial content to lysosomes. Our results provide insight into the interplay between mitochondrial quality control mechanisms and mitochondria-driven immune responses. Mitochondrial content in extracellular vesicles (EVs) can enhance inflammation, although its role in noninflammatory conditions is unclear. Here, the authors show that mitochondria-derived vesicles target material to EVs, whereas Parkin directs damaged mitochondrial content to lysosomes, providing insight into mitochondria-driven immune responses.
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SourceType-Scholarly Journals-1
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-21984-w