Assessment of variability in the plasma 7k SomaScan proteomics assay

SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we pres...

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Veröffentlicht in:Scientific reports Jg. 12; H. 1; S. 17147 - 12
Hauptverfasser: Candia, Julián, Daya, Gulzar N., Tanaka, Toshiko, Ferrucci, Luigi, Walker, Keenan A.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 13.10.2022
Nature Publishing Group
Nature Portfolio
Schlagworte:
631/114/2784
631/1647/2067
631/61/475
Aptamers
Biomarkers - metabolism
Datasets
Human subjects
Humanities and Social Sciences
Humans
multidisciplinary
Normal distribution
Performance assessment
Principal components analysis
Proteins
Proteomics
Proteomics - methods
Science
Science (multidisciplinary)
ISSN:2045-2322, 2045-2322
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-22116-0