Assessment of variability in the plasma 7k SomaScan proteomics assay
SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we pres...
Gespeichert in:
| Veröffentlicht in: | Scientific reports Jg. 12; H. 1; S. 17147 - 12 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
London
Nature Publishing Group UK
13.10.2022
Nature Publishing Group Nature Portfolio |
| Schlagworte: | |
| ISSN: | 2045-2322, 2045-2322 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users. |
|---|---|
| AbstractList | SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users. SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users. Abstract SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users. |
| ArticleNumber | 17147 |
| Author | Daya, Gulzar N. Tanaka, Toshiko Candia, Julián Ferrucci, Luigi Walker, Keenan A. |
| Author_xml | – sequence: 1 givenname: Julián surname: Candia fullname: Candia, Julián email: julian.candia@nih.gov organization: Translational Gerontology Branch, National Institute on Aging, National Institutes of Health – sequence: 2 givenname: Gulzar N. surname: Daya fullname: Daya, Gulzar N. organization: Laboratory of Behavioral Neuroscience, National Institute on Aging, National Institutes of Health – sequence: 3 givenname: Toshiko surname: Tanaka fullname: Tanaka, Toshiko organization: Translational Gerontology Branch, National Institute on Aging, National Institutes of Health – sequence: 4 givenname: Luigi surname: Ferrucci fullname: Ferrucci, Luigi organization: Translational Gerontology Branch, National Institute on Aging, National Institutes of Health – sequence: 5 givenname: Keenan A. surname: Walker fullname: Walker, Keenan A. organization: Laboratory of Behavioral Neuroscience, National Institute on Aging, National Institutes of Health |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36229504$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9ks1u3CAUhVGVqknTvEAXlaVuunEL12DDplKU9CdSpC7SrtEFw4SpbabgiTRvXyZO0ySLsAHBdw6Hy31NDqY4OULeMvqR0UZ-ypwJJWsKUAMw1tb0BTkCykUNDcDBg_UhOcl5TcsQoDhTr8hh0wIoQfkROT_N2eU8ummuoq9uMAU0YQjzrgpTNV-7ajNgHrHqfldXccQri1O1SXF2cQw2V5gz7t6Qlx6H7E7u5mPy6-uXn2ff68sf3y7OTi9rKzida3QGEZU0rUTuKEMqTEnlgXdC0rbvwPbYUGi58L231siuMcw44T1Aq6A5JheLbx9xrTcpjJh2OmLQtxsxrTSmOdjBaasAO9oY3zLkKKj0nPass9gb5ozZe31evDZbM7relgIkHB6ZPj6ZwrVexRutRMuY5MXgw51Bin-2Ls96DNm6YcDJxW3W0IFgqmuVKuj7J-g6btNUSrWnOAclO1modw8T3Uf591kFgAWwKeacnL9HGNX7ptBLU-jSFPq2KTQtIvlEZMOMc4j7V4XheWmzSHO5Z1q59D_2M6q_h3rK1g |
| CitedBy_id | crossref_primary_10_1182_blood_2024025923 crossref_primary_10_3389_fcvm_2023_1123682 crossref_primary_10_1021_jasms_3c00035 crossref_primary_10_1016_j_jtct_2023_10_012 crossref_primary_10_1016_j_tibtech_2023_06_007 crossref_primary_10_3390_biom13020215 crossref_primary_10_1038_s43587_024_00693_1 crossref_primary_10_1136_heartjnl_2025_326091 crossref_primary_10_3390_ijms24031899 crossref_primary_10_1016_j_bbi_2024_07_002 crossref_primary_10_1080_14789450_2024_2431272 crossref_primary_10_1111_cen_14904 crossref_primary_10_1016_j_advnut_2023_09_002 crossref_primary_10_1177_22143602241304990 crossref_primary_10_1038_s41598_024_81277_2 crossref_primary_10_3390_cancers15102672 crossref_primary_10_7554_eLife_86136 crossref_primary_10_1016_j_jtha_2023_07_008 crossref_primary_10_1038_s43587_025_00877_3 crossref_primary_10_3390_healthcare13172133 crossref_primary_10_1097_HC9_0000000000000766 crossref_primary_10_1038_s41591_024_03039_x crossref_primary_10_3390_neurosci6020050 crossref_primary_10_1016_j_arbres_2024_07_007 crossref_primary_10_1080_14789450_2023_2295861 crossref_primary_10_3390_ijms25074082 crossref_primary_10_1002_alz_70261 crossref_primary_10_1016_j_ebiom_2025_105858 crossref_primary_10_1016_j_vaccine_2024_126484 crossref_primary_10_1038_s43587_024_00639_7 crossref_primary_10_1159_000530795 crossref_primary_10_1513_AnnalsATS_202210_857OC crossref_primary_10_1371_journal_pone_0309677 crossref_primary_10_1126_sciadv_adt7176 crossref_primary_10_1186_s12882_025_04166_4 crossref_primary_10_1111_liv_70325 crossref_primary_10_1038_s43587_024_00682_4 crossref_primary_10_1007_s00281_022_00982_0 crossref_primary_10_1002_ange_202412810 crossref_primary_10_1089_ipm_09_06_04 crossref_primary_10_3389_fonc_2024_1417459 crossref_primary_10_1038_s43587_025_00965_4 crossref_primary_10_1002_alz_13676 crossref_primary_10_1002_jcph_70077 crossref_primary_10_3390_jcm14134600 crossref_primary_10_1093_jalm_jfac145 crossref_primary_10_1002_alz_70579 crossref_primary_10_1038_s41586_023_06802_1 crossref_primary_10_1007_s43657_023_00153_7 crossref_primary_10_1371_journal_pmed_1004464 crossref_primary_10_1080_17576180_2024_2437418 crossref_primary_10_1038_s41593_025_01972_9 crossref_primary_10_1055_a_2484_0836 crossref_primary_10_1016_j_ebiom_2025_105800 crossref_primary_10_1161_STROKEAHA_123_043908 crossref_primary_10_3390_ijms26010286 crossref_primary_10_1161_CIRCHEARTFAILURE_124_012434 crossref_primary_10_1016_j_jchf_2023_05_031 crossref_primary_10_1038_s41597_024_03485_9 crossref_primary_10_1016_j_numecd_2024_01_017 crossref_primary_10_3390_ijms25063319 crossref_primary_10_1016_j_jpba_2023_115803 crossref_primary_10_1093_nsr_nwaf278 crossref_primary_10_1038_s41467_025_56935_2 crossref_primary_10_1002_alz_70505 crossref_primary_10_1186_s12931_025_03312_8 crossref_primary_10_1146_annurev_immunol_090122_042631 crossref_primary_10_1021_acs_jproteome_4c01114 crossref_primary_10_1136_ard_2024_225868 crossref_primary_10_1158_1078_0432_CCR_24_3952 crossref_primary_10_3389_fimmu_2025_1528465 crossref_primary_10_1038_s41467_023_42834_x crossref_primary_10_1186_s13024_024_00757_1 crossref_primary_10_1002_pmic_13983 crossref_primary_10_1080_14789450_2024_2350938 crossref_primary_10_1002_anie_202412810 crossref_primary_10_3389_fimmu_2024_1471168 crossref_primary_10_1002_ana_26817 crossref_primary_10_1038_s41591_025_03833_1 crossref_primary_10_1186_s12916_024_03249_7 crossref_primary_10_1186_s13024_025_00874_5 crossref_primary_10_1186_s40364_023_00510_8 crossref_primary_10_1016_j_ajog_2024_03_012 crossref_primary_10_1161_HYPERTENSIONAHA_124_24490 crossref_primary_10_1016_j_jtha_2025_04_020 crossref_primary_10_1038_s41591_025_03890_6 crossref_primary_10_1161_CIRCHEARTFAILURE_124_012343 crossref_primary_10_3390_ijms24010769 crossref_primary_10_59761_RCR5116 crossref_primary_10_3390_nu15030781 crossref_primary_10_3390_pathogens13100867 crossref_primary_10_1038_s41591_025_03563_4 crossref_primary_10_1038_s41598_024_67843_8 crossref_primary_10_1093_rheumatology_keaf311 crossref_primary_10_1126_scitranslmed_adn3504 crossref_primary_10_1038_s41467_024_48259_4 |
| Cites_doi | 10.1371/journal.pone.0015004 10.1002/pmic.201900278 10.1111/acel.12799 10.3389/fonc.2019.01150 10.1186/s40364-017-0112-9 10.2215/CJN.11700921 10.1038/s41591-019-0665-2 10.1038/s41598-018-26640-w 10.1371/journal.pone.0095192 10.1038/s41467-021-27164-0 10.1126/science.aaq1327 10.1038/s41598-017-14755-5 10.1373/jalm.2018.028621 10.5334/jors.166 10.1038/s41586-018-0175-2 10.1373/jalm.2018.027086 10.1038/mtna.2014.49 10.1186/1477-5956-10-13 10.1126/science.abj1541 10.1038/s43587-021-00064-0 10.1038/s41591-019-0673-2 10.3155/1047-3289.59.9.1032 10.1126/sciadv.abi8178 10.1016/B978-0-12-821595-1.00007-5 |
| ContentType | Journal Article |
| Copyright | This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 – notice: 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. – notice: This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | C6C AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA |
| DOI | 10.1038/s41598-022-22116-0 |
| DatabaseName | Springer Nature OA Free Journals CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Biological Science Collection ProQuest Health & Medical Collection Medical Database Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE - Academic Publicly Available Content Database MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2045-2322 |
| EndPage | 12 |
| ExternalDocumentID | oai_doaj_org_article_c92a703bf61a4a508f40d17cadb1ebb2 PMC9561184 36229504 10_1038_s41598_022_22116_0 |
| Genre | Journal Article Research Support, N.I.H., Intramural |
| GrantInformation_xml | – fundername: National Institute on Aging grantid: ZIAAG000971-14; ZIAAG000349-01 funderid: http://dx.doi.org/10.13039/100000049 – fundername: National Institutes of Health (NIH) – fundername: Intramural NIH HHS grantid: ZIA AG000971 – fundername: Intramural NIH HHS grantid: ZIA AG000349 – fundername: ; – fundername: ; grantid: ZIAAG000971-14; ZIAAG000349-01 |
| GroupedDBID | 0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD ABDBF ABUWG ACGFS ACSMW ACUHS ADBBV ADRAZ AENEX AEUYN AFKRA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU DIK DWQXO EBD EBLON EBS ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RNT RNTTT RPM SNYQT UKHRP AASML AAYXX AFFHD AFPKN CITATION PHGZM PHGZT PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM PUEGO 7XB 8FK K9. PKEHL PQEST PQUKI PRINS Q9U 7X8 5PM |
| ID | FETCH-LOGICAL-c540t-aebaaa98b68a4e01a05b000f2475806d72cda302645fdfccb873b1be5ff226923 |
| IEDL.DBID | BENPR |
| ISICitedReferencesCount | 129 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000867889200009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2045-2322 |
| IngestDate | Mon Nov 10 04:34:42 EST 2025 Tue Nov 04 02:07:16 EST 2025 Sat Nov 01 14:39:08 EDT 2025 Tue Oct 07 07:44:21 EDT 2025 Thu Aug 28 04:23:42 EDT 2025 Tue Nov 18 20:58:51 EST 2025 Sat Nov 29 02:07:18 EST 2025 Fri Feb 21 02:36:47 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| License | 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c540t-aebaaa98b68a4e01a05b000f2475806d72cda302645fdfccb873b1be5ff226923 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://www.proquest.com/docview/2724429878?pq-origsite=%requestingapplication% |
| PMID | 36229504 |
| PQID | 2724429878 |
| PQPubID | 2041939 |
| PageCount | 12 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_c92a703bf61a4a508f40d17cadb1ebb2 pubmedcentral_primary_oai_pubmedcentral_nih_gov_9561184 proquest_miscellaneous_2725197699 proquest_journals_2724429878 pubmed_primary_36229504 crossref_primary_10_1038_s41598_022_22116_0 crossref_citationtrail_10_1038_s41598_022_22116_0 springer_journals_10_1038_s41598_022_22116_0 |
| PublicationCentury | 2000 |
| PublicationDate | 2022-10-13 |
| PublicationDateYYYYMMDD | 2022-10-13 |
| PublicationDate_xml | – month: 10 year: 2022 text: 2022-10-13 day: 13 |
| PublicationDecade | 2020 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London – name: England |
| PublicationTitle | Scientific reports |
| PublicationTitleAbbrev | Sci Rep |
| PublicationTitleAlternate | Sci Rep |
| PublicationYear | 2022 |
| Publisher | Nature Publishing Group UK Nature Publishing Group Nature Portfolio |
| Publisher_xml | – name: Nature Publishing Group UK – name: Nature Publishing Group – name: Nature Portfolio |
| References | Kukova (CR19) 2019; 4 Tuli (CR25) 2012; 10 Tin (CR14) 2019; 4 Välikangas, Suomi, Elo (CR26) 2016; 19 Lopez-Silva (CR20) 2022; 17 Sun (CR5) 2018; 558 Williams (CR8) 2019; 25 Pietzner (CR9) 2021; 374 Rohloff (CR3) 2014; 3 CR10 Cheung (CR27) 2017; 5 Armbruster, Pry (CR22) 2008; 29 Lim (CR18) 2017; 5 CR2 Emilsson (CR4) 2018; 361 Lehallier (CR7) 2019; 25 Gold (CR1) 2010; 5 Hyslop, White (CR17) 2009; 59 Reed, Lynn, Meade (CR21) 2002; 9 Pietzner (CR16) 2021; 12 Kim (CR13) 2018; 8 Assarsson (CR23) 2014; 9 Graumann (CR24) 2019; 9 Candia (CR12) 2017; 7 Walker (CR11) 2021; 1 Raffield (CR15) 2020; 20 Tanaka (CR6) 2018; 17 B Lehallier (22116_CR7) 2019; 25 L Gold (22116_CR1) 2010; 5 E Assarsson (22116_CR23) 2014; 9 K Walker (22116_CR11) 2021; 1 T Välikangas (22116_CR26) 2016; 19 N Hyslop (22116_CR17) 2009; 59 T Tanaka (22116_CR6) 2018; 17 22116_CR2 M Pietzner (22116_CR9) 2021; 374 C Kim (22116_CR13) 2018; 8 JC Rohloff (22116_CR3) 2014; 3 L Kukova (22116_CR19) 2019; 4 V Emilsson (22116_CR4) 2018; 361 L Raffield (22116_CR15) 2020; 20 J Candia (22116_CR12) 2017; 7 S Williams (22116_CR8) 2019; 25 S Lim (22116_CR18) 2017; 5 D Armbruster (22116_CR22) 2008; 29 G Reed (22116_CR21) 2002; 9 B Sun (22116_CR5) 2018; 558 M Pietzner (22116_CR16) 2021; 12 A Tin (22116_CR14) 2019; 4 F Cheung (22116_CR27) 2017; 5 J Graumann (22116_CR24) 2019; 9 22116_CR10 C Lopez-Silva (22116_CR20) 2022; 17 L Tuli (22116_CR25) 2012; 10 |
| References_xml | – volume: 5 year: 2010 ident: CR1 article-title: Aptamer-based multiplexed proteomic technology for biomarker discovery publication-title: PLoS ONE doi: 10.1371/journal.pone.0015004 – volume: 20 start-page: 1900278 year: 2020 ident: CR15 article-title: Comparison of proteomic assessment methods in multiple cohort studies publication-title: Proteomics doi: 10.1002/pmic.201900278 – volume: 17 year: 2018 ident: CR6 article-title: Plasma proteomic signature of age in healthy humans publication-title: Aging Cell doi: 10.1111/acel.12799 – volume: 9 start-page: 1150 year: 2019 ident: CR24 article-title: Multi-platform affinity proteomics identify proteins linked to metastasis and immune suppression in ovarian cancer plasma publication-title: Front. Oncol. doi: 10.3389/fonc.2019.01150 – volume: 5 start-page: 32 year: 2017 ident: CR18 article-title: Evaluation of two high-throughput proteomic technologies for plasma biomarker discovery in immunotherapy- treated melanoma patients publication-title: Biomark. Res. doi: 10.1186/s40364-017-0112-9 – volume: 17 start-page: 350 year: 2022 end-page: 360 ident: CR20 article-title: Comparison of aptamer-based and antibody-based assays for protein quantification in chronic kidney disease publication-title: CJASN doi: 10.2215/CJN.11700921 – ident: CR2 – volume: 25 start-page: 1851 year: 2019 end-page: 1857 ident: CR8 article-title: Plasma protein patterns as comprehensive indicators of health publication-title: Nat. Med. doi: 10.1038/s41591-019-0665-2 – volume: 29 start-page: S49 issue: Suppl 1 year: 2008 end-page: 52 ident: CR22 article-title: Limit of blank, limit of detection and limit of quantitation publication-title: Clin. Biochem. Rev. – volume: 8 start-page: 8382 year: 2018 ident: CR13 article-title: Stability and reproducibility of proteomic profiles measured with an aptamer-based platform publication-title: Sci. Rep. doi: 10.1038/s41598-018-26640-w – volume: 9 year: 2014 ident: CR23 article-title: Homogenous 96-plex pea immunoassay exhibiting high sensitivity, specificity, and excellent scalability publication-title: PLoS ONE doi: 10.1371/journal.pone.0095192 – ident: CR10 – volume: 12 start-page: 6822 year: 2021 ident: CR16 article-title: Synergistic insights into human health from aptamer- and antibody-based proteomic profiling publication-title: Nat. Commun. doi: 10.1038/s41467-021-27164-0 – volume: 361 start-page: 769 year: 2018 end-page: 773 ident: CR4 article-title: Co-regulatory networks of human serum proteins link genetics to disease publication-title: Science doi: 10.1126/science.aaq1327 – volume: 7 start-page: 14248 year: 2017 ident: CR12 article-title: Assessment of variability in the Somascan assay publication-title: Sci. Rep. doi: 10.1038/s41598-017-14755-5 – volume: 4 start-page: 331 year: 2019 end-page: 342 ident: CR19 article-title: Comparison of urine and plasma biomarker concentrations measured by aptamer-based versus immunoassay methods in cardiac surgery patients publication-title: J. Appl. Lab. Med. doi: 10.1373/jalm.2018.028621 – volume: 5 start-page: 20 year: 2017 ident: CR27 article-title: Web tool for navigating and plotting SomaLogic ADAT files publication-title: J. Open Res. Softw. doi: 10.5334/jors.166 – volume: 558 start-page: 73 year: 2018 ident: CR5 article-title: Genomic atlas of the human plasma proteome publication-title: Nature doi: 10.1038/s41586-018-0175-2 – volume: 4 start-page: 30 year: 2019 end-page: 39 ident: CR14 article-title: Reproducibility and variability of protein analytes measured using a multiplexed modified aptamer assay publication-title: J. Appl. Lab. Med. doi: 10.1373/jalm.2018.027086 – volume: 3 year: 2014 ident: CR3 article-title: Nucleic acid ligands with protein-like side chains: Modified aptamers and their use as diagnostic and therapeutic agents publication-title: Mol. Therapy Nucl. Acids doi: 10.1038/mtna.2014.49 – volume: 10 start-page: 13 year: 2012 ident: CR25 article-title: Using a spike-in experiment to evaluate analysis of lc-ms data publication-title: Proteome Sci. doi: 10.1186/1477-5956-10-13 – volume: 374 start-page: 839 year: 2021 ident: CR9 article-title: Mapping the proteo-genomic convergence of human diseases publication-title: Science doi: 10.1126/science.abj1541 – volume: 1 start-page: 473 year: 2021 end-page: 489 ident: CR11 article-title: Large-scale plasma proteomic analysis identifies proteins and pathways associated with dementia risk publication-title: Nat. Aging doi: 10.1038/s43587-021-00064-0 – volume: 9 start-page: 1235 year: 2002 end-page: 1239 ident: CR21 article-title: Use of coefficient of variation in assessing variability of quantitative assays publication-title: Clin. Diagn. Lab. Immunol. – volume: 25 start-page: 1843 year: 2019 end-page: 1850 ident: CR7 article-title: Undulating changes in human plasma proteome profiles across the lifespan publication-title: Nat. Med. doi: 10.1038/s41591-019-0673-2 – volume: 59 start-page: 1032 year: 2009 end-page: 1039 ident: CR17 article-title: Estimating precision using duplicate measurements publication-title: J. Air Waste Manag. Assoc. doi: 10.3155/1047-3289.59.9.1032 – volume: 19 start-page: 1 year: 2016 end-page: 11 ident: CR26 article-title: A systematic evaluation of normalization methods in quantitative label-free proteomics publication-title: Brief. Bioinform. – volume: 9 start-page: 1150 year: 2019 ident: 22116_CR24 publication-title: Front. Oncol. doi: 10.3389/fonc.2019.01150 – volume: 4 start-page: 30 year: 2019 ident: 22116_CR14 publication-title: J. Appl. Lab. Med. doi: 10.1373/jalm.2018.027086 – volume: 5 year: 2010 ident: 22116_CR1 publication-title: PLoS ONE doi: 10.1371/journal.pone.0015004 – volume: 558 start-page: 73 year: 2018 ident: 22116_CR5 publication-title: Nature doi: 10.1038/s41586-018-0175-2 – ident: 22116_CR10 doi: 10.1126/sciadv.abi8178 – volume: 9 start-page: 1235 year: 2002 ident: 22116_CR21 publication-title: Clin. Diagn. Lab. Immunol. – volume: 1 start-page: 473 year: 2021 ident: 22116_CR11 publication-title: Nat. Aging doi: 10.1038/s43587-021-00064-0 – volume: 17 start-page: 350 year: 2022 ident: 22116_CR20 publication-title: CJASN doi: 10.2215/CJN.11700921 – volume: 59 start-page: 1032 year: 2009 ident: 22116_CR17 publication-title: J. Air Waste Manag. Assoc. doi: 10.3155/1047-3289.59.9.1032 – volume: 7 start-page: 14248 year: 2017 ident: 22116_CR12 publication-title: Sci. Rep. doi: 10.1038/s41598-017-14755-5 – volume: 8 start-page: 8382 year: 2018 ident: 22116_CR13 publication-title: Sci. Rep. doi: 10.1038/s41598-018-26640-w – volume: 29 start-page: S49 issue: Suppl 1 year: 2008 ident: 22116_CR22 publication-title: Clin. Biochem. Rev. – volume: 5 start-page: 32 year: 2017 ident: 22116_CR18 publication-title: Biomark. Res. doi: 10.1186/s40364-017-0112-9 – volume: 3 year: 2014 ident: 22116_CR3 publication-title: Mol. Therapy Nucl. Acids doi: 10.1038/mtna.2014.49 – volume: 9 year: 2014 ident: 22116_CR23 publication-title: PLoS ONE doi: 10.1371/journal.pone.0095192 – volume: 5 start-page: 20 year: 2017 ident: 22116_CR27 publication-title: J. Open Res. Softw. doi: 10.5334/jors.166 – volume: 25 start-page: 1843 year: 2019 ident: 22116_CR7 publication-title: Nat. Med. doi: 10.1038/s41591-019-0673-2 – volume: 4 start-page: 331 year: 2019 ident: 22116_CR19 publication-title: J. Appl. Lab. Med. doi: 10.1373/jalm.2018.028621 – volume: 10 start-page: 13 year: 2012 ident: 22116_CR25 publication-title: Proteome Sci. doi: 10.1186/1477-5956-10-13 – volume: 17 year: 2018 ident: 22116_CR6 publication-title: Aging Cell doi: 10.1111/acel.12799 – volume: 374 start-page: 839 year: 2021 ident: 22116_CR9 publication-title: Science doi: 10.1126/science.abj1541 – volume: 19 start-page: 1 year: 2016 ident: 22116_CR26 publication-title: Brief. Bioinform. – volume: 12 start-page: 6822 year: 2021 ident: 22116_CR16 publication-title: Nat. Commun. doi: 10.1038/s41467-021-27164-0 – volume: 361 start-page: 769 year: 2018 ident: 22116_CR4 publication-title: Science doi: 10.1126/science.aaq1327 – volume: 20 start-page: 1900278 year: 2020 ident: 22116_CR15 publication-title: Proteomics doi: 10.1002/pmic.201900278 – ident: 22116_CR2 doi: 10.1016/B978-0-12-821595-1.00007-5 – volume: 25 start-page: 1851 year: 2019 ident: 22116_CR8 publication-title: Nat. Med. doi: 10.1038/s41591-019-0665-2 |
| SSID | ssj0000529419 |
| Score | 2.6585126 |
| Snippet | SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of... Abstract SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of... |
| SourceID | doaj pubmedcentral proquest pubmed crossref springer |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 17147 |
| SubjectTerms | 631/114/2784 631/1647/2067 631/61/475 Aptamers Biomarkers - metabolism Datasets Human subjects Humanities and Social Sciences Humans multidisciplinary Normal distribution Performance assessment Principal components analysis Proteins Proteomics Proteomics - methods Science Science (multidisciplinary) |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEB5KaKGX0qQvJ2lQobfWRJJlSzqmj5BTCKSF3IQkS3Rp4w3ZTWD_fUeS1832eenVkmH4NNJ8gzTfALzWyqbAKWvNfVOnU7J2Tnd1aEKgvlOpbi83m5Cnp-riQp_dafWV3oQVeeAC3KHX3KJXutgxKyzSiShoz6S3vWPBuXz6Iuu5k0wVVW-uBdNjlQxt1OECI1WqJsPci2PSg2n0RiTKgv2_Y5m_Ppb86cY0B6Ljx_BoZJDkqFi-DffCsAMPSk_J1RP4cDRpbZJ5JLeYCxcp7hWZDQTpHrlCwnxpifxKzueX9hyxJVmtIdUnLwiSabt6Cp-PP356f1KPrRJqj5RrWdvgrLVauU5ZESiztE3hPXKB-QDtesl9bxvMt0Qb--i9U7JxzIU2RuRfSPKewdYwH8ILIMw5pjxH5t21Qnhp-w4nS4Q0st7RvgK2hs34UUc8tbP4ZvJ9dqNMgdog1CZDbWgFb6Z_roqKxl9nv0urMc1MCtj5A_qFGf3C_MsvKthfr6UZt-XCcIlshmslVQWvpmHcUOmWxA5hfpPnpGLeTusKnpelnyzBaM91S0UFcsMpNkzdHBlmX7Jodyogxmy6grdr9_lh1p-h2P0fUOzBQ578Pr3DafZha3l9E17CfX-7nC2uD_LG-Q6RvRnk priority: 102 providerName: Directory of Open Access Journals |
| Title | Assessment of variability in the plasma 7k SomaScan proteomics assay |
| URI | https://link.springer.com/article/10.1038/s41598-022-22116-0 https://www.ncbi.nlm.nih.gov/pubmed/36229504 https://www.proquest.com/docview/2724429878 https://www.proquest.com/docview/2725197699 https://pubmed.ncbi.nlm.nih.gov/PMC9561184 https://doaj.org/article/c92a703bf61a4a508f40d17cadb1ebb2 |
| Volume | 12 |
| WOSCitedRecordID | wos000867889200009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: DOA dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M~E dateStart: 20110101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M7P dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: 7X7 dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Proquest Central Journals customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: BENPR dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: PIMPY dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M2P dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB7RXZB64U0JlJWRuEHUxHnYPqEWWsGhq4iCtJws23HKCposm22l_feMnWyq5dELFx8SR3I8Hs83Hs83AK8EV85wslBQk4Rulwy1FnloE2sjk3OXt-eLTbDplM9mougP3Nr-WuVmT_QbddkYd0Z-QBkaIooeMn-7-Bm6qlEuutqX0NiBsWMqS0cwPjqeFp-GUxYXx0pj0WfLRAk_aNFiuawy9MEofoPu9JZF8sT9f0Obf16a_C1y6g3Syb3__ZX7cLeHouSwWzsP4JatH8Kdrjjl-hG8PxxIO0lTkSt0qjtO7zWZ1wRxI1kg8r5QhH0nZ82FOkMhEU_74BKdW4KoXK0fw5eT48_vPoR9zYXQIHZbhcpqpZTgOucqtVGsoszhhIqm6FhEecmoKVWCjluaVWVljOYs0bG2WVUhkEO0-ARGdVPbp0BirWNuKEL4PEtTw1SZY2eGMqniUkdlAPFm3qXpCcldXYwf0gfGEy47WUmUlfSyklEAr4dvFh0dx429j5w4h56OSts_aJbnstdMaQRVuO3pKo9VqhCvVmlUxsyoUsdWaxrA_kaKstfvVl6LMICXw2vUTBduUbVtLn0flxWcCxHAXrd2hpEgbKAii9IA2Naq2hrq9pt6_s2zf7tMZHTLA3izWX_Xw_r3VDy7-S-ewy51KuGu6iT7MFotL-0LuG2uVvN2OYEdNmO-5ZNewyb-8ALbU1q4lmE7Lj6eFl9_ARbbLsY |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFH6qCggu7EuggJHgBFFjJxPbB4QKpWrVMkJqkebm2o5DR6XJMDMtmj_Fb-TZWaph6a0HrokjOfH3li9vA3gphfaGk8eS2TT2WjI2RuaxS51LbC583V4YNsGHQzEayc8r8LOrhfFplZ1ODIq6qK3_R77OOBoihgxZvJt8j_3UKB9d7UZoNLDYdYsfSNlmb3c28XxfMbb18eDDdtxOFYgteifzWDujtZbC5EJnLqE6GXhLWLIMXeckLzizhU6RmmSDsiitNYKnhho3KEt0VaRvdIAq_wruhfsUMj7i_T8dHzXLqGxrc5JUrM_QPvoaNmR8DKkWkvcl-xfGBPzNt_0zRfO3OG0wf1u3_rcPdxtuto422Wgk4w6suOouXGtGby7uweZG35KU1CU50yiIIU94QcYVQa-YTJBXnGjCj8l-faL3EYIkNLXwZdwzgpxDL-7Dl0t5hQewWtWVewSEGkOFZUhQ8kGWWa6LHBdzxEBJC5MUEdDunJVt2637qR_fVAj7p0I12FCIDRWwoZIIXvfPTJpmIxeufu_h06_0jcLDhXr6VbV6R1nJNCp1U-ZUZxq98TJLCsqtLgx1xrAI1jrUqFZ7zdQ5ZCJ40d9GveODSbpy9WlY42uecykjeNhgtd8JOkVMDpIsAr6E4qWtLt-pxkeht7mvs6YCn3zT4f18W__-FI8vfovncH374NOe2tsZ7j6BG8yLo09KStdgdT49dU_hqj2bj2fTZ0GeCRxethz8AjU4hPs |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFLaqsogL-xIoYCQ4QTSxs9g-IFQYRlSF0UgFqTfXdmw6Kk2GmWnR_DV-Hc_OUg1Lbz1wTRzJjr-3fPZbEHouuPKGk8WCmjT2WjLWWhSxTa1NTMF93l5oNsHGY76_LyYb6GeXC-PDKjudGBR1WRt_Rj6gDAwRBYbMB64Ni5gMR29m32PfQcrftHbtNBqI7NrVD6Bvi9c7Q9jrF5SO3n9-9yFuOwzEBjyVZaysVkoJrguuMpsQleTeKjqagRudFCWjplQp0JQsd6UzRnOWaqJt7hy4LcIXPQD1f4llee6l6xOd9Oc7_gYtI6LN00lSPliArfT5bMD-KNAuIPJrtjC0DPibn_tnuOZvd7bBFI5u_M8_8Sa63jrgeLuRmFtow1a30ZWmJefqDhpu96VKce3wqQIBDfHDKzytMHjLeAZ841hhdoT36mO1B9DEodiFT-9eYOAianUXfbmQJdxDm1Vd2QcIE60JNxSIS5FnmWGqLGAwAzw4UuqkjBDp9lyatgy77wbyTYZwgJTLBicScCIDTmQSoZf9N7OmCMm5o996KPUjfQHx8KCef5WtPpJGUAXKXruCqEyBl-6ypCTMqFITqzWN0FaHINlqtYU8g0-EnvWvQR_5SyZV2fokjPG50IUQEbrf4LafCThLVORJFiG2hui1qa6_qaaHoea5z78mHL581WH_bFr__hUPz1_FU3QV4C8_7ox3H6Fr1Eumj1VKt9Dmcn5iH6PL5nQ5XcyfBNHG6OCixeAXmzqNyA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Assessment+of+variability+in+the+plasma+7k+SomaScan+proteomics+assay&rft.jtitle=Scientific+reports&rft.au=Candia%2C+Juli%C3%A1n&rft.au=Daya%2C+Gulzar+N.&rft.au=Tanaka%2C+Toshiko&rft.au=Ferrucci%2C+Luigi&rft.date=2022-10-13&rft.pub=Nature+Publishing+Group+UK&rft.eissn=2045-2322&rft.volume=12&rft.issue=1&rft_id=info:doi/10.1038%2Fs41598-022-22116-0&rft.externalDocID=10_1038_s41598_022_22116_0 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon |