Identification and characterization of a SARS-CoV-2 specific CD8+ T cell response with immunodominant features

The COVID-19 pandemic caused by SARS-CoV-2 is a continuous challenge worldwide, and there is an urgent need to map the landscape of immunogenic and immunodominant epitopes recognized by CD8 + T cells. Here, we analyze samples from 31 patients with COVID-19 for CD8 + T cell recognition of 500 peptide...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Nature communications Ročník 12; číslo 1; s. 2593 - 14
Hlavní autoři:	Gangaev, Anastasia, Ketelaars, Steven L. C., Isaeva, Olga I., Patiwael, Sanne, Dopler, Anna, Hoefakker, Kelly, De Biasi, Sara, Gibellini, Lara, Mussini, Cristina, Guaraldi, Giovanni, Girardis, Massimo, Ormeno, Cami M. P. Talavera, Hekking, Paul J. M., Lardy, Neubury M., Toebes, Mireille, Balderas, Robert, Schumacher, Ton N., Ovaa, Huib, Cossarizza, Andrea, Kvistborg, Pia
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 10.05.2021 Nature Publishing Group Nature Portfolio
Témata:	38/1 38/91 49/31 631/250/1619/554/1834 631/250/2152 631/250/255 Adult Aged Aged, 80 and over Alleles Antigens CD8 antigen CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - pathology Cell activation Cell migration Cell recognition Convalescence Coronaviruses COVID-19 COVID-19 - immunology COVID-19 - pathology Cytokines Epitopes Epitopes, T-Lymphocyte - immunology Female Gene expression Histocompatibility antigen HLA Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class I - immunology Humanities and Social Sciences Humans Immune response Immunodominance Immunodominant Epitopes - chemistry Immunodominant Epitopes - immunology Immunogenicity Immunologic Memory Immunological memory Lymphocyte Activation Lymphocytes Lymphocytes T Male Memory cells Middle Aged multidisciplinary Pandemics Polyproteins - immunology SARS-CoV-2 - immunology Science Science (multidisciplinary) Severe acute respiratory syndrome coronavirus 2 Viral diseases Viral Proteins - immunology
ISSN:	2041-1723, 2041-1723
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	The COVID-19 pandemic caused by SARS-CoV-2 is a continuous challenge worldwide, and there is an urgent need to map the landscape of immunogenic and immunodominant epitopes recognized by CD8 + T cells. Here, we analyze samples from 31 patients with COVID-19 for CD8 + T cell recognition of 500 peptide-HLA class I complexes, restricted by 10 common HLA alleles. We identify 18 CD8 + T cell recognized SARS-CoV-2 epitopes, including an epitope with immunodominant features derived from ORF1ab and restricted by HLA-A*01:01. In-depth characterization of SARS-CoV-2-specific CD8 + T cell responses of patients with acute critical and severe disease reveals high expression of NKG2A, lack of cytokine production and a gene expression profile inhibiting T cell re-activation and migration while sustaining survival. SARS-CoV-2-specific CD8 + T cell responses are detectable up to 5 months after recovery from critical and severe disease, and these responses convert from dysfunctional effector to functional memory CD8 + T cells during convalescence. Many viral antigens have been identified in patients with COVID-19 patients, but which of these result in meaningful immune responses is unclear. Here the authors identify a range of SARS-CoV-2 CD8 + T cell responses across patients including a response targeting an epitope of ORF1ab with immunodominant properties.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-021-22811-y