Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through multiple...

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Veröffentlicht in:Scientific reports Jg. 7; H. 1; S. 4369 - 13
Hauptverfasser: Sjollema, Jelmer, van der Mei, Henny C., Hall, Connie L., Peterson, Brandon W., de Vries, Joop, Song, Lei, Jong, Ed D. de, Busscher, Henk J., Swartjes, Jan J. T. M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 29.06.2017
Nature Publishing Group
Nature Portfolio
Schlagworte:
631/57/2282
639/766/25
Adhesion
Atomic force microscopy
Bacteria
Bacterial Adhesion
Bacterial Physiological Phenomena
Biomechanical Phenomena
Computer Simulation
Data processing
Focal Adhesions
Humanities and Social Sciences
Microscopy, Atomic Force
Models, Biological
multidisciplinary
Science
Science (multidisciplinary)
ISSN:2045-2322, 2045-2322
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Zusammenfassung:Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through multiple, reversibly-binding tethers that detach and successively re-attach, but not collectively detach to cause detachment of an entire bacterium. Arguments build on combining analyses of confined Brownian-motion of bacteria adhering to glass and their AFM force-distance curves and include the following observations: (1) force-distance curves showed detachment events indicative of multiple binding tethers, (2) vibration amplitudes of adhering bacteria parallel to a surface decreased with increasing adhesion-forces acting perpendicular to the surface, (3) nanoscopic displacements of bacteria with relatively long autocorrelation times up to several seconds, in absence of microscopic displacement, (4) increases in Mean-Squared-Displacement over prolonged time periods according to t α with 0 < α ≪ 1, indicative of confined displacement. Analysis of simulated position-maps of adhering particles using a new, in silico model confirmed that adhesion to surfaces is irreversible through detachment and successive re-attachment of reversibly-binding tethers. This makes bacterial adhesion mechanistically comparable with the irreversible adsorption of high-molecular-weight proteins to surfaces, mediated by multiple, reversibly-binding molecular segments.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-04703-8