Dynamic FRET-FLIM based screening of signal transduction pathways

Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative method to screen for protein–protein interactions and is frequently used to record the outcome of signal transduction events. With new highly sensitive and photon efficient FLIM instrumentation, the technique also becomes attracti...

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Bibliographic Details
Published in:	Scientific reports Vol. 11; no. 1; pp. 20711 - 13
Main Authors:	Harkes, Rolf, Kukk, Olga, Mukherjee, Sravasti, Klarenbeek, Jeffrey, van den Broek, Bram, Jalink, Kees
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 20.10.2021 Nature Publishing Group Nature Portfolio
Subjects:	631/114/1314 631/114/2163 631/114/2391 631/114/2400 631/45/731 631/57/2267 631/61/191/505 631/80/2373 631/80/86 Cell activation Cell Line, Tumor Cell signaling Cell surface Cyclic AMP Cyclic AMP - metabolism Energy transfer Fluorescence Fluorescence resonance energy transfer Fluorescence Resonance Energy Transfer - methods HeLa Cells Humanities and Social Sciences Humans Instrumentation Isoforms Microscopy, Fluorescence - methods multidisciplinary Optical Imaging - methods Phosphoric Diester Hydrolases - metabolism Photons Protein interaction Proteins - metabolism Science Science (multidisciplinary) Signal transduction Signal Transduction - physiology
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
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