Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it is challenging to profile Cas9 activity in the endogenous cellular environment. Here we leverage our TRIP technology to integrate ~ 1k barcoded repor...

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Bibliographic Details
Published in:	Nature communications Vol. 10; no. 1; pp. 1598 - 14
Main Authors:	Gisler, Santiago, Gonçalves, Joana P., Akhtar, Waseem, de Jong, Johann, Pindyurin, Alexey V., Wessels, Lodewyk F. A., van Lohuizen, Maarten
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 08.04.2019 Nature Publishing Group Nature Portfolio
Subjects:	38/39 38/47 38/70 45/41 631/1647/1513/1967/3196 631/1647/2017/1958 631/337/176 631/337/4041 Animals Cell Line CRISPR CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA - genetics DNA Mutational Analysis DNA repair Efficiency Embryo cells Gene Editing - methods Gene sequencing Genes, Reporter - genetics Genetic Loci - genetics Genetic modification Genetic Vectors - genetics Genome editing Genomes Genomics gRNA Humanities and Social Sciences Loci Lymphocytes B Mice Mouse Embryonic Stem Cells multidisciplinary Mutation Mutation Rate Nucleotide sequence Plasmids - genetics Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics Science Science (multidisciplinary) Stem cell transplantation Stem cells
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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