Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue
Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberratio...
Uloženo v:
| Vydáno v: | Neurophotonics (Print) Ročník 8; číslo 3; s. 035001 |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
Bellingham
Society of Photo-Optical Instrumentation Engineers
01.07.2021
S P I E - International Society for Society of Photo-optical Instrumentation Engineers (SPIE) |
| Témata: | |
| ISSN: | 2329-423X, 2329-4248 |
| On-line přístup: | Získat plný text |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue.
Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth.
Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices.
Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μm inside the biological tissue and obtain a 60% signal increase after correction.
Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue. |
|---|---|
| AbstractList | Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue. Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth. Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices. Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μ m inside the biological tissue and obtain a 60% signal increase after correction. Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue.Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue. Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth. Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices. Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μ m inside the biological tissue and obtain a 60% signal increase after correction. Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue. Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue. Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth. Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices. Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μm inside the biological tissue and obtain a 60% signal increase after correction. Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue. Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue.Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth.Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices.Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μm inside the biological tissue and obtain a 60% signal increase after correction.Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue. Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that is highly sensitive to optical aberrations, limiting its depth penetration. Therefore, there is a need for methods to reduce optical aberrations and improve the spatial resolution of STED microscopy inside thick biological tissue. Aim: The aim of our work was to develop and validate a method based on adaptive optics to achieve an a priori correction of spherical aberrations as a function of imaging depth. Approach: We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. We then used a spatial light modulator to apply corrective phase shifts and validate this calibration approach by imaging neurons in living brain slices. Results: After quantifying the spatial resolution in depth in phantom samples, we demonstrated that the corrections can substantially increase image quality in living brain slices. Specifically, we could measure structures as small as 80 nm at a depth of 90 μm inside the biological tissue and obtain a 60% signal increase after correction. Conclusion: We propose a simple and robust approach to calibrate and compensate the distortions of the STED beam profile introduced by spherical aberrations with increasing imaging depth and demonstrated that this method offers significant improvements in microscopy performance for nanoscale cellular imaging in live tissue. We demonstrate an approach based on adaptive optics to improve the spatial resolution of STED microscopy inside thick biological tissue by a priori correction of spherical aberrations as a function of imaging depth. We first measured the aberrations in a phantom sample of gold and fluorescent nanoparticles suspended in an agarose gel with a refractive index closely matching living brain tissue. Using a spatial light modulator to apply corrective phase shifts, we imaged neurons in living brain slices and show that the corrections can substantially increase image quality. Specifically, we could measure structures as small as 80 nm at a depth of 90 μ m inside the biological tissue, and obtain a 60% signal increase after correction. |
| Author | Mercier, Luc Murana, Emanuele Bancelin, Stéphane Nägerl, U. Valentin |
| Author_xml | – sequence: 1 givenname: Stéphane orcidid: 0000-0001-6328-0423 surname: Bancelin fullname: Bancelin, Stéphane email: stephane.bancelin@u-bordeaux.fr organization: University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France – sequence: 2 givenname: Luc surname: Mercier fullname: Mercier, Luc email: luc.mercier@u-bordeaux.fr organization: University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France – sequence: 3 givenname: Emanuele surname: Murana fullname: Murana, Emanuele email: emanuele.murana@gmail.com organization: University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France – sequence: 4 givenname: U. Valentin orcidid: 0000-0001-6831-9008 surname: Nägerl fullname: Nägerl, U. Valentin email: stephane.bancelin@u-bordeaux.fr organization: University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France |
| BackLink | https://hal.science/hal-03390366$$DView record in HAL |
| BookMark | eNp9kUFr3DAQhUVJadJt7j0aemkPdiWN7ZUvhSWkTWFJcmihNyHL410F23IleSH_vvI6pHShOUkafe9pNO8tORvsgIS8ZzRjjK0_s-z2fp-JDDIKBaXsFbngwKs057k4e97Dr3Ny6f0DjQRnVcHgDTmHnEFZiOqCuE2Nzqlg7JBo6xzq49YMiQ-mnzoVsEmwN97P5QbHDo9Ab7SzXtvxMQk24tqh8piYXu3MsJvBsJ9dOnOYz7VT8RCizYTvyOtWdR4vn9YV-fn1-sfVTbq9-_b9arNNdQFVSBWt25YqVEVdYwnANJSai5KtFXBdsbxhStct51qItVKipg3TVEDTYKPavIQV-bL4jlPdY6NxCE51cnSxSfcorTLy35vB7OXOHqTglELJosGnxWB_IrvZbOVcowBVJMvDzH58eszZ3xP6IOPMNHadGtBOXvIiRlGsRZSsyIcT9MFOboijkPF7HEQh2GxIF2qes3fYPnfAqJzzl0zG_KWQIJf8o6Q8kWgTjtHG75nuJWG6CP1o8G83_-X_ANQzxl0 |
| CitedBy_id | crossref_primary_10_1016_j_molcel_2021_12_022 crossref_primary_10_1371_journal_pone_0290550 crossref_primary_10_3390_photonics10020178 crossref_primary_10_1038_s41596_024_01132_6 crossref_primary_10_1523_JNEUROSCI_1125_22_2022 crossref_primary_10_1063_5_0075012 crossref_primary_10_3389_fncel_2023_1243633 crossref_primary_10_1073_pnas_2422020121 crossref_primary_10_1002_glia_24103 crossref_primary_10_1117_1_NPh_11_1_014415 |
| Cites_doi | 10.1016/j.bpj.2012.12.053 10.1523/JNEUROSCI.09-08-02982.1989 10.1364/OL.33.000113 10.1515/nanoph-2018-0133 10.1364/OE.16.020774 10.1073/pnas.0604965103 10.1016/S0896-6273(00)00084-2 10.1016/j.bpj.2012.12.054 10.3389/neuro.01.1.1.010.2007 10.1088/2050-6120/3/2/024002 10.1002/lpor.201500151 10.1038/nature14467 10.1063/1.5020249 10.1016/j.ymeth.2020.01.020 10.1152/physrev.00012.2013 10.1364/OE.20.020998 10.1038/nn.3682 10.1364/BOE.10.001999 10.1364/OE.27.023378 10.1002/jbio.201300041 10.1111/j.1365-2818.2009.03188.x 10.1093/jmicro/dfv033 10.1021/acs.chemrev.6b00653 10.1126/science.1137395 10.1111/j.1365-2818.1998.99999.x 10.1088/1361-6463/ab6f1b 10.1016/j.neuron.2009.07.011 10.1046/j.1365-2818.2002.01004.x 10.1364/OE.17.001714 10.1016/j.bpj.2011.07.027 10.1088/0031-9155/47/12/305 10.1364/OE.20.001084 10.1364/OE.14.001339 10.1126/science.1215369 10.1117/12.227816 10.1364/OE.24.008862 10.7554/eLife.34700 10.1038/lsa.2014.46 10.1016/j.cell.2018.02.007 10.1038/nmeth.2019 |
| ContentType | Journal Article |
| Copyright | The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. 2021. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2021 The Authors. Distributed under a Creative Commons Attribution 4.0 International License 2021 The Authors 2021 The Authors |
| Copyright_xml | – notice: The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. – notice: 2021. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2021 The Authors. – notice: Distributed under a Creative Commons Attribution 4.0 International License – notice: 2021 The Authors 2021 The Authors |
| DBID | AAYXX CITATION 8FE 8FH ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI 7X8 1XC VOOES 5PM |
| DOI | 10.1117/1.NPh.8.3.035001 |
| DatabaseName | CrossRef ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Database AUTh Library subscriptions: ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central ProQuest Central Student SciTech Premium Collection Biological Sciences Biological Science Database ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Biological Science Collection ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) ProQuest One Academic ProQuest One Academic (New) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| EISSN | 2329-4248 |
| EndPage | 035001 |
| ExternalDocumentID | PMC8200361 oai:HAL:hal-03390366v1 10_1117_1_NPh_8_3_035001 |
| GrantInformation_xml | – fundername: Era-net NEURON grantid: ANR-17-NEU3-0005-01 – fundername: ANR grantid: ANR-17-CE37-0011 – fundername: FRM grantid: DEQ20160334901 – fundername: European Union’s Horizon 2020 – fundername: Marie Sklodowska-Curie grantid: 794492 |
| GroupedDBID | 0R 4.4 AAPBV ACGFS ADACO ADBBV ALMA_UNASSIGNED_HOLDINGS BCNDV EBS FQ0 GROUPED_DOAJ M4X O9- OK1 RPM SPBNH UT2 0R~ 53G AAFWJ AAYXX ADMLS AFFHD AFKRA AFPKN AKROS BBNVY BENPR BHPHI CCPQU CITATION HCIFZ M7P PBYJJ PHGZM PHGZT PIMPY PQGLB 8FE 8FH ABUWG AZQEC DWQXO GNUQQ LK8 PKEHL PQEST PQQKQ PQUKI 7X8 PUEGO 1XC VOOES 5PM |
| ID | FETCH-LOGICAL-c539t-a0bff0aea5bbe6331c36c28617a32c914d1acbf22c887aa8b0d1c083ddedaf463 |
| IEDL.DBID | M7P |
| ISICitedReferencesCount | 21 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000704179300002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2329-423X |
| IngestDate | Tue Nov 04 01:56:13 EST 2025 Tue Oct 14 20:48:50 EDT 2025 Thu Sep 04 16:00:40 EDT 2025 Fri Jul 25 11:34:50 EDT 2025 Sat Nov 29 06:22:44 EST 2025 Tue Nov 18 22:06:24 EST 2025 Sat Oct 02 10:56:51 EDT 2021 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | aberration correction stimulated emission depletion microscopy brain slice imaging |
| Language | English |
| License | Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c539t-a0bff0aea5bbe6331c36c28617a32c914d1acbf22c887aa8b0d1c083ddedaf463 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0001-6328-0423 0000-0001-6831-9008 |
| OpenAccessLink | https://www.proquest.com/docview/2862385811?pq-origsite=%requestingapplication% |
| PMID | 34136589 |
| PQID | 2862385811 |
| PQPubID | 6522123 |
| PageCount | 1 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_8200361 proquest_journals_2862385811 hal_primary_oai_HAL_hal_03390366v1 proquest_miscellaneous_2542357803 crossref_primary_10_1117_1_NPh_8_3_035001 crossref_citationtrail_10_1117_1_NPh_8_3_035001 spie_journals_10_1117_1_NPh_8_3_035001 |
| ProviderPackageCode | FQ0 SPBNH UT2 |
| PublicationCentury | 2000 |
| PublicationDate | 2021-07-01 |
| PublicationDateYYYYMMDD | 2021-07-01 |
| PublicationDate_xml | – month: 07 year: 2021 text: 2021-07-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Bellingham |
| PublicationPlace_xml | – name: Bellingham |
| PublicationTitle | Neurophotonics (Print) |
| PublicationTitleAlternate | Neurophoton |
| PublicationYear | 2021 |
| Publisher | Society of Photo-Optical Instrumentation Engineers S P I E - International Society for Society of Photo-optical Instrumentation Engineers (SPIE) |
| Publisher_xml | – name: Society of Photo-Optical Instrumentation Engineers – name: S P I E - International Society for – name: Society of Photo-optical Instrumentation Engineers (SPIE) |
| References | r2 r3 r4 r5 r6 r7 r8 r9 r30 r10 r32 r31 r12 r34 r11 r33 r14 r36 r13 r35 r16 r38 r15 r37 r18 r17 r39 r19 r40 r21 r20 r23 r22 r25 r24 r27 r26 r29 r28 r1 |
| References_xml | – ident: r13 doi: 10.1016/j.bpj.2012.12.053 – ident: r34 doi: 10.1523/JNEUROSCI.09-08-02982.1989 – ident: r20 doi: 10.1364/OL.33.000113 – ident: r24 doi: 10.1515/nanoph-2018-0133 – ident: r6 doi: 10.1364/OE.16.020774 – ident: r19 doi: 10.1073/pnas.0604965103 – ident: r28 doi: 10.1016/S0896-6273(00)00084-2 – ident: r14 doi: 10.1016/j.bpj.2012.12.054 – ident: r35 doi: 10.3389/neuro.01.1.1.010.2007 – ident: r12 doi: 10.1088/2050-6120/3/2/024002 – ident: r15 doi: 10.1002/lpor.201500151 – ident: r37 doi: 10.1038/nature14467 – ident: r8 doi: 10.1063/1.5020249 – ident: r30 doi: 10.1016/j.ymeth.2020.01.020 – ident: r33 doi: 10.1152/physrev.00012.2013 – ident: r11 doi: 10.1364/OE.20.020998 – ident: r38 doi: 10.1038/nn.3682 – ident: r22 doi: 10.1364/BOE.10.001999 – ident: r23 doi: 10.1364/OE.27.023378 – ident: r7 doi: 10.1002/jbio.201300041 – ident: r9 doi: 10.1111/j.1365-2818.2009.03188.x – ident: r17 doi: 10.1093/jmicro/dfv033 – ident: r2 doi: 10.1021/acs.chemrev.6b00653 – ident: r1 doi: 10.1126/science.1137395 – ident: r31 doi: 10.1111/j.1365-2818.1998.99999.x – ident: r16 doi: 10.1088/1361-6463/ab6f1b – ident: r3 doi: 10.1016/j.neuron.2009.07.011 – ident: r32 doi: 10.1046/j.1365-2818.2002.01004.x – ident: r10 doi: 10.1364/OE.17.001714 – ident: r4 doi: 10.1016/j.bpj.2011.07.027 – ident: r40 doi: 10.1088/0031-9155/47/12/305 – ident: r27 doi: 10.1364/OE.20.001084 – ident: r26 doi: 10.1364/OE.14.001339 – ident: r5 doi: 10.1126/science.1215369 – ident: r39 doi: 10.1117/12.227816 – ident: r21 doi: 10.1364/OE.24.008862 – ident: r36 doi: 10.7554/eLife.34700 – ident: r18 doi: 10.1038/lsa.2014.46 – ident: r25 doi: 10.1016/j.cell.2018.02.007 – ident: r29 doi: 10.1038/nmeth.2019 |
| SSID | ssj0001219513 |
| Score | 2.3142202 |
| Snippet | Significance: Stimulated emission depletion (STED) microscopy enables nanoscale imaging of live samples, but it requires a specific spatial beam shaping that... We demonstrate an approach based on adaptive optics to improve the spatial resolution of STED microscopy inside thick biological tissue by a priori correction... |
| SourceID | pubmedcentral hal proquest crossref spie |
| SourceType | Open Access Repository Aggregation Database Enrichment Source Index Database Publisher |
| StartPage | 035001 |
| SubjectTerms | Approximation Astigmatism Brain slice preparation Engineering Sciences Lasers Microscopy Morphology Nanoparticles Neuroimaging Optics Photonic Photonics Polynomials Research Papers Spatial discrimination |
| Title | Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue |
| URI | http://www.dx.doi.org/10.1117/1.NPh.8.3.035001 https://www.proquest.com/docview/2862385811 https://www.proquest.com/docview/2542357803 https://hal.science/hal-03390366 https://pubmed.ncbi.nlm.nih.gov/PMC8200361 |
| Volume | 8 |
| WOSCitedRecordID | wos000704179300002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2329-4248 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001219513 issn: 2329-423X databaseCode: DOA dateStart: 20190101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2329-4248 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001219513 issn: 2329-423X databaseCode: M7P dateStart: 20190101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2329-4248 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001219513 issn: 2329-423X databaseCode: BENPR dateStart: 20190101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2329-4248 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001219513 issn: 2329-423X databaseCode: PIMPY dateStart: 20190101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9NAEB6RlAMXXgURKNGCUCUOTrze-HVCAbUqUoksBFI4rXbXa8VS44TErdR_z8zaThMkeuFm2ePHemZn57XzAXwoQm6oy4iXByrxJoXBORelykuoeVxI_ph2G4Uv49ksmc_TrA24bduyyk4nOkWdrwzFyMcBmt6UxOL80_q3R6hRlF1tITR6cERdEoQr3cv2YiwcDQjh8OWC1EPLYd5lKnk85qNZthglIzGi7FqLCtOtTL0F1UXuGZ1_l0z2t-vS7i1F50_-dxBP4XFrhLJpIzXP4IGtnsPxtEIHfHnLTpkrC3Xx9mPYTLXdNGLCDEF5uI0QrKwYKoclgX_ZnBFoHIXdWG7X1M0bj5ZU6Ud7Xm5ZvUJysk63lpVLB4tEhPWCnnJVUkiDaYKqYLUTgxfw8_zsx5cLrwVq8Ewo0tpTvi4KX1kVam0jIbgRkcGx8liJwKR8knNldBEEBlWaUon2c27Q9kPVmqtiEomX0K9WlX0FTBWRNWGUo9pDV8-kGs0rHSVGoB_LhU4HMO6YJE3bxZzANK5k483Ekktkq0ykkA1bB_Bxd8e66eBxD-175PuOjFpvX0wvJZ3zhUhxtY9ukOikY65sJ_xW3nF2AO92l_HnU_5FVXZ1jTThxDUX8sUA4gNxOnjj4ZWqXLim3wlVEUb49FMSvLsX_2sor-__yjfwKKDqHFd4fAL9enNt38JDc4OM3gyhF8-TIRx9Pptl34cuTjF0UwvPZV-_Zb_-AHptK3w |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEB61KRJceBVEoMCCoBIHJ1lv7NgHhCKgStQ0yqFI4bTsrtdKpMYJiVuUP8VvZGZtpwkSvfXAzbLH69c345md2fkA3qUBN9RlxEt8FXnt1KDOhbHyImoeF1A8pt1C4UFnOIzG43i0B7-rtTBUVlnZRGeok7mhOfKmj643JbE4_7T46RFrFGVXKwqNAhandv0LQ7bVx_4X_L7vff_k6_nnnleyCngmEHHuqZZO05ayKtDahkJwI0KDo_OOEr6JeTvhyujU9w3qn1KRbiXcoKOCdiBRaTsUOO4-HLQR7FENDkb9s9H3rVkdji6LcIx2fuyhrzKucqO80-SN4WjSiBqiQfm8koem-hfuT6gSc8vN_btIs7ZaTO3Wz-_kwf_22h7C_dLNZt1CLx7Bns0ew2E3U_l8tmbHzBW-uozCISy72i4LRWCGyErcUg82zRiavxnRm9mEES0eTSyyxC6oXzluzaiWkVb1rFk-R3Hyv1eWTWeO-IkE8wmNcjGlSRumiYyD5Q7oT-DbrTz9U6hl88w-A6bS0JogTNCwYzBrYo0OpA4jIzBS50LHdWhWoJCm7NNOdCEXsojXOpJLhJGMpJAFjOrwYXPGouhRcoPsW8TZRoyai_e6A0n7WkLE6M-EVyh0VIFJliZtJa-RVIc3m8P48inDpDI7v0SZoO3aJ7VEHTo78N254u6RbDpxbc0jqpMMcfRjAvr1hf_1KM9vvsvXcLd3fjaQg_7w9AXc86kWyZVZH0EtX17al3DHXOFHX74qlZjBj9tWgD-TJYf- |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF61KUJceBVEoMCCoBIHJ1mvnweEIkrUqCHKAaRw2u6u14qlxg6JW5S_xq9jZm2nCRK99cDNsie7sf3NeGZndj5C3qU-09hlxElcGTleqkHnglg6ETaP8zEeU3aj8Cgcj6PpNJ7skd_NXhgsq2xsojXUSaFxjbzrguuNSSzGumldFjE5GXxa_HSQQQozrQ2dRgWRM7P-BeHb6uPwBN71e9cdfPn2-dSpGQYc7fO4dGRPpWlPGukrZQLOmeaBhplYKLmrY-YlTGqVuq4GXZQyUr2EaXBawCYkMvUCDuPukwNwyT3QsYPJ8Ovkx9YKDwP3hVt2Ozd2wG-ZNnlSFnZZZzyZdaIO72Bur-akab6L-zOsytxyef8u2GytFpnZ-hAOHvzPj_AhuV-737Rf6csjsmfyx-Swn8uymK_pMbUFsTbTcEiWfWWWlYJQjSQmdgsIzXIKZnGOtGcmoUiXhwuONDEL7GMOR3OsccTdPmtaFiCOfvnK0GxuCaFQsJzhKBcZLuZQhSQdtLQK8IR8v5W7f0paeZGbZ4TKNDDaDxIw-BDk6liBY6mCSHOI4BlXcZt0G4AIXfdvRxqRC1HFcaFgAiAlIsFFBak2-bD5xaLqXXKD7FvA3EYMm46f9kcCz_U4j8HPCa5A6KgBlqhN3Upco6pN3mwuw8PHzJPMTXEJMr5n2yr1eJuEO1DemXH3Sp7NbLvzCOsnAxj9GEF_PfG_buX5zf_yNbkLqBej4fjsBbnnYomSrb4-Iq1yeWlekjv6Ct758lWtz5Sc3zb-_wCMZZC- |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Aberration+correction+in+stimulated+emission+depletion+microscopy+to+increase+imaging+depth+in+living+brain+tissue&rft.jtitle=Neurophotonics+%28Print%29&rft.au=Bancelin%2C+St%C3%A9phane&rft.au=Mercier%2C+Luc&rft.au=Murana%2C+Emanuele&rft.au=N%C3%A4gerl%2C+U.+Valentin&rft.date=2021-07-01&rft.pub=Society+of+Photo-optical+Instrumentation+Engineers+%28SPIE%29&rft.issn=2329-423X&rft.eissn=2329-4248&rft.volume=8&rft.issue=3&rft_id=info:doi/10.1117%2F1.NPh.8.3.035001&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=oai%3AHAL%3Ahal-03390366v1 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2329-423X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2329-423X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2329-423X&client=summon |