Genomic epidemiology of Mycobacterium tuberculosis in Santa Catarina, Southern Brazil

Mycobacterium tuberculosis ( M.tb ), the pathogen responsible for tuberculosis (TB) poses as the major cause of death among infectious diseases. The knowledge about the molecular diversity of M.tb enables the implementation of more effective surveillance and control measures and, nowadays, Whole Gen...

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Bibliographic Details
Published in:Scientific reports Vol. 10; no. 1; p. 12891
Main Authors: Verza, Mirela, Scheffer, Mara Cristina, Salvato, Richard Steiner, Schorner, Marcos André, Barazzetti, Fernando Hartmann, Machado, Hanalydia de Melo, Medeiros, Taiane Freitas, Rovaris, Darcita Buerger, Portugal, Isabel, Viveiros, Miguel, Perdigão, João, Kritski, Afrânio, Bazzo, Maria Luiza
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 30.07.2020
Nature Publishing Group
Subjects:
631/326
631/326/107
631/326/325
631/337
Adult
Antibiotics
Antitubercular Agents - pharmacology
Brazil - epidemiology
Disease transmission
Drug resistance
Epidemiology
Ethambutol
Ethionamide
Female
Genomes
Humanities and Social Sciences
Humans
Infectious diseases
Isoniazid
Male
Metropolitan areas
Middle Aged
Molecular Epidemiology
multidisciplinary
Multidrug resistance
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - pathogenicity
Phylogeny
Pyrazinamide
Rifampin
Science
Science (multidisciplinary)
Streptomycin
Tuberculosis
Tuberculosis, Multidrug-Resistant - epidemiology
Tuberculosis, Multidrug-Resistant - genetics
Tuberculosis, Multidrug-Resistant - transmission
Whole Genome Sequencing
Brazil
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Mycobacterium tuberculosis ( M.tb ), the pathogen responsible for tuberculosis (TB) poses as the major cause of death among infectious diseases. The knowledge about the molecular diversity of M.tb enables the implementation of more effective surveillance and control measures and, nowadays, Whole Genome Sequencing (WGS) holds the potential to produce high-resolution epidemiological data in a high-throughput manner. Florianópolis, the state capital of Santa Catarina (SC) in south Brazil, shows a high TB incidence (46.0/100,000). Here we carried out a WGS-based evaluation of the M.tb strain diversity, drug-resistance and ongoing transmission in the capital metropolitan region. Resistance to isoniazid, rifampicin, streptomycin was identified respectively in 4.0% (n = 6), 2.0% (n = 3) and 1.3% (n = 2) of the 151 studied strains by WGS. Besides, resistance to pyrazinamide and ethambutol was detected in 0.7% (n = 1) and reistance to ethionamide and fluoroquinolone (FQ) in 1.3% (n = 2), while a single (0.7%) multidrug-resistant (MDR) strain was identified. SNP-based typing classified all isolates into M.tb Lineage 4, with high proportion of sublineages LAM (60.3%), T (16.4%) and Haarlem (7.9%). The average core-genome distance between isolates was 420.3 SNPs, with 43.7% of all isolates grouped across 22 genomic clusters thereby showing the presence of important ongoing TB transmission events. Most clusters were geographically distributed across the study setting which highlights the need for an urgent interruption of these large transmission chains. The data conveyed by this study shows the presence of important and uncontrolled TB transmission in the metropolitan area and provides precise data to support TB control measures in this region.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-69755-9