Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost

The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uri...

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Vydáno v:Journal of virology Ročník 71; číslo 10; s. 7681
Hlavní autoři: Mjaaland, S, Rimstad, E, Falk, K, Dannevig, B H
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.10.1997
Témata:
Anemia - veterinary
Anemia - virology
Animals
Atlantic Ocean
Cell Line
Centrifugation, Density Gradient
Cloning, Molecular
Fish Diseases
Genome, Viral
Molecular Weight
Orthomyxoviridae - classification
Orthomyxoviridae - genetics
Orthomyxoviridae - isolation & purification
Orthomyxoviridae Infections - veterinary
Orthomyxoviridae Infections - virology
Polymerase Chain Reaction - methods
RNA, Viral - isolation & purification
Salmon - virology
Atlantic Ocean
ISSN:0022-538X
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Shrnutí:The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uridine-labeled ISAV RNA revealed that the ISAV genome is segmented. The genome consists of eight segments that range from 1.0 to 2.3 kb, with a total molecular size of approximately 14.5 kb. One ISAV-specific molecular clone, corresponding to the smallest genome segment, was obtained by cDNA cloning of mRNA from an ISAV-infected cell culture. This clone gave a positive hybridization signal on Northern blots of pelleted ISAV. Pretreatment of the ISAV pellet with RNase A resulted in the disappearance of the positive hybridization signal, demonstrating that the genome is single stranded. Reverse transcriptase PCR with primers corresponding to sequences from the molecular clone and target RNA from ISAV-infected and noninfected fish tissues gave specific positive reactions. Alignments of the nucleotide sequence of the molecular clone did not reveal significant homology with any other available sequence in databases. However, the data presented here, together with morphological and replicational properties previously described, indicate that ISAV has a strong resemblance to members of the Orthomyxoviridae family. This is the first thoroughly characterized orthomyxo-like virus isolated from a teleost.
Bibliografie:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0022-538X
DOI:10.1128/jvi.71.10.7681-7686.1997