Probing Physical Properties of a DNA-Protein Complex Using Nanofluidic Channels

A method to investigate physical properties of a DNA‐protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double‐stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching...

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Bibliographic Details
Published in:	Small (Weinheim an der Bergstrasse, Germany) Vol. 10; no. 5; pp. 884 - 887
Main Authors:	Frykholm, Karolin, Alizadehheidari, Mohammadreza, Fritzsche, Joachim, Wigenius, Jens, Modesti, Mauro, Persson, Fredrik, Westerlund, Fredrik
Format:	Journal Article
Language:	English
Published:	Germany Blackwell Publishing Ltd 12.03.2014 Wiley Subscription Services, Inc
Subjects:	Biophysical Phenomena Channels Deoxyribonucleic acid DNA DNA - chemistry DNA, Circular - metabolism Filaments fluorescence Handles Joining lipid passivation Lipids Microfluidics - methods nanofluidics Nanofluids Nanostructure Nanotechnology Nanotechnology - methods Physical properties proteins Rec A Recombinases - chemistry lipid passivation fluorescence nanofluidics DNA proteins
ISSN:	1613-6810, 1613-6829, 1613-6829
Online Access:	Get full text
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