Probing Physical Properties of a DNA-Protein Complex Using Nanofluidic Channels

A method to investigate physical properties of a DNA‐protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double‐stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Jg. 10; H. 5; S. 884 - 887
Hauptverfasser: Frykholm, Karolin, Alizadehheidari, Mohammadreza, Fritzsche, Joachim, Wigenius, Jens, Modesti, Mauro, Persson, Fredrik, Westerlund, Fredrik
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Germany Blackwell Publishing Ltd 12.03.2014
Wiley Subscription Services, Inc
Schlagworte:
Biophysical Phenomena
Channels
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA, Circular - metabolism
Filaments
fluorescence
Handles
Joining
lipid passivation
Lipids
Microfluidics - methods
nanofluidics
Nanofluids
Nanostructure
Nanotechnology
Nanotechnology - methods
Physical properties
proteins
Rec A Recombinases - chemistry
lipid passivation
fluorescence
nanofluidics
DNA
proteins
ISSN:1613-6810, 1613-6829, 1613-6829
Online-Zugang:Volltext
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Zusammenfassung:A method to investigate physical properties of a DNA‐protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double‐stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.
Bibliographie:ArticleID:SMLL201302028
Swedish Research Council - No. 2011-4324
Chalmers Area of Advance in Nanoscience and Nanotechnology and the Swedish Foundation for Strategic Research
Agence Nationale pour la Recherche - No. 1521 01
ark:/67375/WNG-6V4XC5PG-Q
istex:E296512C75E8A134BED59E70FA9DE07A74915F21
These authors contributed equally.
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ISSN:1613-6810
1613-6829
1613-6829
DOI:10.1002/smll.201302028