A new eriophyid mite-borne membrane-enveloped virus-like complex isolated from plants

A decade ago, a new mite-transmitted disease was described on wheat ( Triticum aesativum) and maize ( Zea mays) that due to its geographical location was referred to as High Plains Disease (HPD). To determine the etiology, we established colonies of HPD pathogen-transmitting eriophyid wheat curl mit...

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Published in:Virology (New York, N.Y.) Vol. 347; no. 2; pp. 343 - 353
Main Authors: Skare, Joanna M., Wijkamp, Ineke, Denham, Ivey, Rezende, Jorge A.M., Kitajima, Elliot W., Park, Jong-Won, Desvoyes, Benedicte, Rush, Charles M., Michels, Gerald, Scholthof, Karen-Beth G., Scholthof, Herman B.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 10.04.2006
Subjects:
32-kDa protein
Aceria tosichella
amino acid sequences
Animals
Arachnid Vectors - pathogenicity
Arachnid Vectors - virology
complementary DNA
corn
disease vectors
Eriophyid mite
High Plains wheat mosaic emaravirus
HPD
Maize
mites
Mites - pathogenicity
Mites - virology
molecular cloning
Mosaic Viruses - isolation & purification
Mosaic Viruses - pathogenicity
negative sense, single-stranded RNA viruses
Plant
Plant Diseases - parasitology
Plant Diseases - virology
Plant Viruses - isolation & purification
Plant Viruses - pathogenicity
Plants
RNA
Triticum
Triticum aestivum
Virus
Wheat
Zea mays
Zea mays - virology
Virus
Plant
RNA
Eriophyid mite
Maize
32-kDa protein
HPD
Wheat
ISSN:0042-6822, 1096-0341
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Summary:A decade ago, a new mite-transmitted disease was described on wheat ( Triticum aesativum) and maize ( Zea mays) that due to its geographical location was referred to as High Plains Disease (HPD). To determine the etiology, we established colonies of HPD pathogen-transmitting eriophyid wheat curl mites ( Aceria tosichella) on wheat plants for maintenance of a continuous source of infected material. Analyses of nucleic acid obtained from infected plants showed the presence of HPD-specific RNAs ranging from 1.5 to 8 kilobases, but comparisons between the sequence of cDNAs and the databases did not reveal any clear identity with known viruses. We demonstrate that a diagnostic HPD-specific 32-kDa protein that accumulates in plants is encoded by a small RNA species (RNA-s). Upon infestation of upper wheat parts with viruliferous mites, the RNA-s encoded protein becomes detectable within a few days in the roots, indicative of an effective virus-like mode of transport. Membranous particles, resembling those observed in thin sections of infected plants, were isolated and shown to envelope a thread-like ribonucleoprotein complex containing the RNA-s encoded 32-kDa protein. This complex was associated with single-stranded (−)-sense RNAs, whereas free (+)-sense RNA was only detected in total RNA of infected plants. Based on the collective properties, we conclude that HPD is caused by a newly emerged mite-borne virus, for which we propose the name Maize red stripe virus (MRStV).
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ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2005.11.030