Automated immobilized metal affinity chromatography system for enrichment of Escherichia coli phosphoproteome

Enrichment of bacterial phosphopeptides is an essential step prior to bottom‐up mass spectrometry‐based analysis of the phosphoproteome, which is fundamental to understanding the role of phosphoproteins in cell signaling and regulation of protein activity. We developed an automated immobilized metal...

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Vydáno v:	Electrophoresis Ročník 34; číslo 11; s. 1619 - 1626
Hlavní autoři:	Qu, Yi, Wu, Si, Zhao, Rui, Zink, Erika, Orton, Daniel J., Moore, Ronald J., Meng, Da, Clauss, Therese R. W., Aldrich, Joshua T., Lipton, Mary S., Paša-Tolić, Ljiljana
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Germany Blackwell Publishing Ltd 01.06.2013
Témata:	Affinity Amino Acid Sequence Automated Bacteria Chromatography Chromatography, Affinity - instrumentation Electrophoresis Enrichment Equipment Design Escherichia coli Escherichia coli - chemistry Escherichia coli Proteins - chemistry Escherichia coli Proteins - isolation & purification Immobilized metal affinity chromatography enrichment Metals - chemistry Molecular Sequence Data Peptides Phosphopeptides - chemistry Phosphopeptides - isolation & purification Phosphoproteins - chemistry Phosphoproteins - isolation & purification Phosphorylation Proteomics Proteomics - methods
ISSN:	0173-0835, 1522-2683, 1522-2683
On-line přístup:	Získat plný text
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