Automated immobilized metal affinity chromatography system for enrichment of Escherichia coli phosphoproteome

Enrichment of bacterial phosphopeptides is an essential step prior to bottom‐up mass spectrometry‐based analysis of the phosphoproteome, which is fundamental to understanding the role of phosphoproteins in cell signaling and regulation of protein activity. We developed an automated immobilized metal...

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Bibliographic Details
Published in:	Electrophoresis Vol. 34; no. 11; pp. 1619 - 1626
Main Authors:	Qu, Yi, Wu, Si, Zhao, Rui, Zink, Erika, Orton, Daniel J., Moore, Ronald J., Meng, Da, Clauss, Therese R. W., Aldrich, Joshua T., Lipton, Mary S., Paša-Tolić, Ljiljana
Format:	Journal Article
Language:	English
Published:	Germany Blackwell Publishing Ltd 01.06.2013
Subjects:	Affinity Amino Acid Sequence Automated Bacteria Chromatography Chromatography, Affinity - instrumentation Electrophoresis Enrichment Equipment Design Escherichia coli Escherichia coli - chemistry Escherichia coli Proteins - chemistry Escherichia coli Proteins - isolation & purification Immobilized metal affinity chromatography enrichment Metals - chemistry Molecular Sequence Data Peptides Phosphopeptides - chemistry Phosphopeptides - isolation & purification Phosphoproteins - chemistry Phosphoproteins - isolation & purification Phosphorylation Proteomics Proteomics - methods
ISSN:	0173-0835, 1522-2683, 1522-2683
Online Access:	Get full text
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