Weak correlation between antibody titers and neutralizing activity in sera from SARS‐CoV‐2 infected subjects

Plenty of serologic tests for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) have been developed so far, thus documenting the importance of evaluating the relevant features of the immune response to this viral agent. The performance of these assays is currently under investigation. Amo...

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Vydáno v:Journal of medical virology Ročník 93; číslo 4; s. 2160 - 2167
Hlavní autoři: Criscuolo, Elena, Diotti, Roberta A., Strollo, Marta, Rolla, Serena, Ambrosi, Alessandro, Locatelli, Massimo, Burioni, Roberto, Mancini, Nicasio, Clementi, Massimo, Clementi, Nicola
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Wiley Subscription Services, Inc 01.04.2021
John Wiley and Sons Inc
Témata:
Algorithms
Animals
Antibodies
Antibodies, Neutralizing
Antibodies, Viral - blood
Chlorocebus aethiops
Comparative analysis
Coronaviridae
Coronaviruses
Correlation analysis
COVID-19
COVID-19 - blood
COVID-19 - epidemiology
COVID-19 - immunology
COVID-19 - virology
COVID-19 Testing - methods
COVID‐19 diagnostic assays
Humans
Immune response
Immunoglobulin G
Immunoglobulin G - blood
Italy - epidemiology
Laboratories
Neutralizing
neutralizing activity
Pandemics
SARS-CoV-2 - immunology
SARS‐CoV‐2 serology
Sensitivity and Specificity
Serologic Tests - methods
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Vaccines
Vero Cells
Viral diseases
Virology
Italy
neutralizing activity
COVID-19 diagnostic assays
SARS-CoV-2 serology
ISSN:0146-6615, 1096-9071, 1096-9071
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Shrnutí:Plenty of serologic tests for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) have been developed so far, thus documenting the importance of evaluating the relevant features of the immune response to this viral agent. The performance of these assays is currently under investigation. Amongst them, LIAISON® SARS‐CoV‐2 S1/S2 IgG by DiaSorin and Elecsys Anti‐SARS‐CoV‐2 cobas® by Roche are currently used by laboratory medicine hospital departments in Italy and many other countries. In the present study, we firstly compared two serologic tests on serum samples collected at two different time points from 46 laboratory‐confirmed coronavirus disease‐2019 (COVID‐19) subjects. Secondly, 85 negative serum samples collected before the SARS‐CoV‐2 pandemic were analyzed. Thirdly, possible correlations between antibody levels and the resulting neutralizing activity against a clinical isolate of SARS‐CoV‐2 were evaluated. Results revealed that both tests are endowed with low sensitivity on the day of hospital admission, which increased to 97.8% and 100% for samples collected after 15 days for DiaSorin and Roche tests, respectively. The specificity evaluated for the two tests ranges from 96.5% to 100%, respectively. Importantly, a poor direct correlation between antibody titers and neutralizing activity levels was evidenced in the present study. These data further shed light on both potentials and possible limitations related to SARS‐CoV‐2 serology. In this context, great efforts are still necessary for investigating antibody kinetics to develop novel diagnostic algorithms. Moreover, further investigations on the role of neutralizing antibodies and their correlate of protection will be of paramount importance for the development of effective vaccines. Highlights A comparative analysis between two serologic assays for the detection of antibodies directed against SARS‐CoV‐2 was carried out on sera from subjects testing positive for SARS‐CoV‐2 nasopharyngeal swabs. Results revealed that both tests are endowed with low sensitivity on the day of hospital admission, which increased for samples collected after 15 days. A poor direct correlation between antibody titers and neutralizing activity levels was evidenced in the present study.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0146-6615
1096-9071
1096-9071
DOI:10.1002/jmv.26605