Age-Related Mechanisms of Cricoid Cartilage Response to Injury in the Developing Rabbit

Objective To evaluate age‐related mechanisms of cricoid cartilage response to injury in an animal model. Study Design A pilot study using monoclonal antibodies to chondrocyte proliferation markers collagen II, aggrecan, and proliferating cell nuclear antigen (PCNA) to evaluate age‐related response o...

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Published in:The Laryngoscope Vol. 113; no. 7; pp. 1145 - 1148
Main Authors: Cherukupally, Shilpa Reddy, Adams, Allison B., Mankarious, Leila A.
Format: Journal Article
Language:English
Published: Hoboken, NJ John Wiley & Sons, Inc 01.07.2003
Wiley-Blackwell
Subjects:
Aggrecans
Aging - metabolism
Aging - pathology
Animals
Biological and medical sciences
Cell Division
chondrocyte proliferation
Chondrocytes - pathology
Chondroitin Sulfate Proteoglycans - analysis
Collagen Type II - analysis
cricoid
Cricoid Cartilage - chemistry
Cricoid Cartilage - injuries
Cricoid Cartilage - pathology
Ent, stomatology, face, injuries. Foreign bodies. Diseases due to physical agents: otorhinolaryngology
Extracellular Matrix Proteins
Immunohistochemistry
Intubation, Intratracheal - adverse effects
Lectins, C-Type
Medical sciences
Proliferating Cell Nuclear Antigen - analysis
Proteoglycans - analysis
rabbit
Rabbits
Subglottic stenosis
Traumas. Diseases due to physical agents
Wound Healing
Animal model
Correlation
Cricoid cartilage
Pathophysiology
Subglottic stenosis
cricoid
Rabbit
chondrocyte proliferation
Lagomorpha
Experimental study
Reaction
Trauma
Mechanism
Vertebrata
Mammalia
ENT disease
Larynx disease
immunohistochemistry
Age
ISSN:0023-852X, 1531-4995
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Summary:Objective To evaluate age‐related mechanisms of cricoid cartilage response to injury in an animal model. Study Design A pilot study using monoclonal antibodies to chondrocyte proliferation markers collagen II, aggrecan, and proliferating cell nuclear antigen (PCNA) to evaluate age‐related response of injured cricoid cartilage. Methods Twenty‐seven New Zealand white rabbits aged 4 weeks, 8 weeks, and 1.5 years were studied. Six animals in each age group underwent intracartilaginous injury through cricofissure and tracheofissure. Three animals of each group were used as control subjects. The animals were killed 4 weeks after injury, their cricoid cartilages harvested, and 7‐μm sections of tissue obtained. The cricoid tissue sections were stained with immunofluorescent monoclonal antibody markers to collagen II, aggrecan, and PCNA. Results In all age groups, no control animals had symptoms of airway compromise or immunohistochemical abnormality. For all three markers of chondrocyte proliferation, the 4‐week‐old animals showed markedly increased staining at the injured edges of cartilage. The 8‐week‐old animals showed mild increased extracellular staining, and the 1.5‐year‐old animals showed no increased staining compared with uninjured areas of the cricoid ring and with control animals. Conclusion There was a progressive, age‐related attenuation of staining for markers of chondrocyte proliferation in the 8‐week and 1.5‐year‐old rabbits compared with the 4‐week‐old rabbit cricoids.
Bibliography:istex:027A0A88CA9385253F9DAD1527ADA2211E7759A2
ark:/67375/WNG-GBNDSJ1T-W
ArticleID:LARY5541130709
Supported by a grant from the Hood Foundation. Presented as a Poster at the Meeting of the American Society of Pediatric Otolaryngology, Scottsdale, AZ, May 9, 2001.
Presented as a Poster at the Meeting of the American Society of Pediatric Otolaryngology, Scottsdale, AZ, May 9, 2001.
Supported by a grant from the Hood Foundation.
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ISSN:0023-852X
1531-4995
DOI:10.1097/00005537-200307000-00009