Single‐cell protein profiling defines cell populations associated with triple‐negative breast cancer aggressiveness

Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single‐cell techniques in combination with next‐...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Molecular oncology Ročník 17; číslo 6; s. 1024 - 1040
Hlavní autoři: Kvokačková, Barbora, Fedr, Radek, Kužílková, Daniela, Stuchlý, Jan, Vávrová, Adéla, Navrátil, Jiří, Fabian, Pavel, Ondruššek, Róbert, Ovesná, Petra, Remšík, Ján, Bouchal, Jan, Kalina, Tomáš, Souček, Karel
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States John Wiley & Sons, Inc 01.06.2023
John Wiley and Sons Inc
Wiley
Témata:
Antibodies
Bar codes
Breast Cancer
Cells
Cloning
Cytometry
Fibroblasts
Glycerol
mass cytometry
Metastasis
Patients
phenotypic plasticity
Proteins
Proteomics
Single-cell protein
single‐cell profiles
Stromal cells
Surgery
triple‐negative breast cancer
Tumor cells
tumor heterogeneity
Tumor microenvironment
Tumors
unsupervised machine learning algorithm
United States > US
Germany
unsupervised machine learning algorithm
triple-negative breast cancer
phenotypic plasticity
mass cytometry
single-cell profiles
tumor heterogeneity
ISSN:1574-7891, 1878-0261, 1878-0261
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single‐cell techniques in combination with next‐generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single‐cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial‐mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor‐associated stroma. Furthermore, in a retrospective tissue‐microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential. The extensive heterogeneity of triple‐negative breast cancer (TNBC) affects disease progression and therapeutic response. To investigate TNBC heterogeneity in more detail, we profiled 26 treatment‐naïve primary TNBC at the single‐cell level using mass cytometry. We introduced a new clinically relevant Ki‐67+LNR index and identified distinct tumor and stromal populations associated with clinical observations.
AbstractList Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single‐cell techniques in combination with next‐generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single‐cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial‐mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor‐associated stroma. Furthermore, in a retrospective tissue‐microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential. The extensive heterogeneity of triple‐negative breast cancer (TNBC) affects disease progression and therapeutic response. To investigate TNBC heterogeneity in more detail, we profiled 26 treatment‐naïve primary TNBC at the single‐cell level using mass cytometry. We introduced a new clinically relevant Ki‐67+LNR index and identified distinct tumor and stromal populations associated with clinical observations.
Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single-cell techniques in combination with next-generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single-cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial-mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor-associated stroma. Furthermore, in a retrospective tissue-microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential.Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single-cell techniques in combination with next-generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single-cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial-mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor-associated stroma. Furthermore, in a retrospective tissue-microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential.
Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single-cell techniques in combination with next-generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single-cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial-mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor-associated stroma. Furthermore, in a retrospective tissue-microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential.
Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single-cell techniques in combination with next-generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single-cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial-mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor-associated stroma. Furthermore, in a retrospective tissue-microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential.
Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic, extensive intratumoral heterogeneity that promotes disease progression and influences drug response. Single‐cell techniques in combination with next‐generation computation provide an unprecedented opportunity to identify molecular events with therapeutic potential. Here, we describe the generation of a comprehensive mass cytometry panel for multiparametric detection of 23 phenotypic markers and 13 signaling molecules. This single‐cell proteomic approach allowed us to explore the landscape of TNBC heterogeneity, with particular emphasis on the tumor microenvironment. We prospectively profiled freshly resected tumors from 26 TNBC patients. These tumors contained phenotypically distinct subpopulations of cancer and stromal cells that were associated with the patient's clinical status at the time of surgery. We further classified the epithelial‐mesenchymal plasticity of tumor cells, and molecularly defined phenotypically diverse populations of tumor‐associated stroma. Furthermore, in a retrospective tissue‐microarray TNBC cohort, we showed that the level of CD97 at the time of surgery has prognostic potential. The extensive heterogeneity of triple‐negative breast cancer (TNBC) affects disease progression and therapeutic response. To investigate TNBC heterogeneity in more detail, we profiled 26 treatment‐naïve primary TNBC at the single‐cell level using mass cytometry. We introduced a new clinically relevant Ki‐67+LNR index and identified distinct tumor and stromal populations associated with clinical observations.
Author Ondruššek, Róbert
Fabian, Pavel
Remšík, Ján
Stuchlý, Jan
Kvokačková, Barbora
Ovesná, Petra
Bouchal, Jan
Kalina, Tomáš
Navrátil, Jiří
Vávrová, Adéla
Fedr, Radek
Souček, Karel
Kužílková, Daniela
AuthorAffiliation 2 International Clinical Research Center St. Anne's University Hospital Brno Czech Republic
7 Department of Comprehensive Cancer Care Masaryk Memorial Cancer Institute Brno Czech Republic
9 Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry Palacký University and University Hospital Olomouc Czech Republic
11 Institute of Biostatistics and Analyses, Faculty of Medicine Masaryk University Brno Czech Republic
3 Department of Experimental Biology, Faculty of Science Masaryk University Brno Czech Republic
4 Childhood Leukaemia Investigation Prague Czech Republic
12 Human Oncology and Pathogenesis Program Memorial Sloan Kettering Cancer Center New York City NY USA
6 Faculty of Science Charles University Prague Czech Republic
5 Department of Pediatric Haematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol Czech Republic
8 Department of Oncological Pathology Masaryk Memor
AuthorAffiliation_xml – name: 2 International Clinical Research Center St. Anne's University Hospital Brno Czech Republic
– name: 9 Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry Palacký University and University Hospital Olomouc Czech Republic
– name: 8 Department of Oncological Pathology Masaryk Memorial Cancer Institute Brno Czech Republic
– name: 11 Institute of Biostatistics and Analyses, Faculty of Medicine Masaryk University Brno Czech Republic
– name: 1 Department of Cytokinetics Institute of Biophysics of the Czech Academy of Sciences Brno Czech Republic
– name: 6 Faculty of Science Charles University Prague Czech Republic
– name: 4 Childhood Leukaemia Investigation Prague Czech Republic
– name: 5 Department of Pediatric Haematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol Czech Republic
– name: 7 Department of Comprehensive Cancer Care Masaryk Memorial Cancer Institute Brno Czech Republic
– name: 10 Department of Pathology EUC Laboratoře CGB a.s. Ostrava Czech Republic
– name: 3 Department of Experimental Biology, Faculty of Science Masaryk University Brno Czech Republic
– name: 12 Human Oncology and Pathogenesis Program Memorial Sloan Kettering Cancer Center New York City NY USA
Author_xml – sequence: 1
  givenname: Barbora
  orcidid: 0000-0002-6478-9923
  surname: Kvokačková
  fullname: Kvokačková, Barbora
  organization: Masaryk University
– sequence: 2
  givenname: Radek
  surname: Fedr
  fullname: Fedr, Radek
  organization: St. Anne's University Hospital
– sequence: 3
  givenname: Daniela
  orcidid: 0000-0001-8086-9790
  surname: Kužílková
  fullname: Kužílková, Daniela
  organization: Charles University Prague and University Hospital Motol
– sequence: 4
  givenname: Jan
  surname: Stuchlý
  fullname: Stuchlý, Jan
  organization: Charles University Prague and University Hospital Motol
– sequence: 5
  givenname: Adéla
  surname: Vávrová
  fullname: Vávrová, Adéla
  organization: Charles University Prague
– sequence: 6
  givenname: Jiří
  surname: Navrátil
  fullname: Navrátil, Jiří
  organization: Masaryk Memorial Cancer Institute
– sequence: 7
  givenname: Pavel
  surname: Fabian
  fullname: Fabian, Pavel
  organization: Masaryk Memorial Cancer Institute
– sequence: 8
  givenname: Róbert
  surname: Ondruššek
  fullname: Ondruššek, Róbert
  organization: EUC Laboratoře CGB a.s
– sequence: 9
  givenname: Petra
  orcidid: 0000-0002-3547-5896
  surname: Ovesná
  fullname: Ovesná, Petra
  organization: Masaryk University
– sequence: 10
  givenname: Ján
  orcidid: 0000-0002-3228-2896
  surname: Remšík
  fullname: Remšík, Ján
  organization: Memorial Sloan Kettering Cancer Center
– sequence: 11
  givenname: Jan
  orcidid: 0000-0003-4842-1720
  surname: Bouchal
  fullname: Bouchal, Jan
  organization: Palacký University and University Hospital
– sequence: 12
  givenname: Tomáš
  orcidid: 0000-0003-4475-2872
  surname: Kalina
  fullname: Kalina, Tomáš
  organization: Charles University Prague and University Hospital Motol
– sequence: 13
  givenname: Karel
  orcidid: 0000-0001-7283-8150
  surname: Souček
  fullname: Souček, Karel
  email: ksoucek@ibp.cz
  organization: Masaryk University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/36550781$$D View this record in MEDLINE/PubMed
BookMark eNqFks1u1DAUhSNURH9gzQ5FYsNmWtvx7wqhqkClQV0Aa8uxb1KPMvZgZzrqjkfgGXkSnElb0UqI1bV8z_l85HuPq4MQA1TVa4xOMULkDEshF4hwfIqbhrNn1dHDzUE5M0EXQip8WB3nvEKIccXVi-qwSBkSEh9Vu68-9AP8_vnLwjDUmxRH8GGqnR9Kq3bQ-QC5nttxsx3M6GPItck5Wm9GcPXOj9f1mPxmDwrQF8kN1G0Ck8fammAh1abvE-RcGgWXX1bPOzNkeHVXT6rvHy--nX9eLK8-XZ5_WC4saxhbSAe2c6IFxJnrWiZbpUBCJ1xjMafAQTiFHLS0tZxLSTvWUkowdZjKthHNSXU5c100K71Jfm3SrY7G6_1FTL02afR2AK0QMVw6KggiVFGQilPjRHmDWyWcLaz3M2uzbdfgLIQxmeER9HEn-GvdxxuNESmTwLQQ3t0RUvyxhTzqtc_Tz5oAcZs1EUxipKgkRfr2iXQVtymUv9JEEtqUiFwV1Zu_Iz1kuR9wEbBZYFPMOUGnrR_3EywJ_VCi6WmR9LQ2elobvV-k4jt74rtH_9vBZ8fOD3D7P7n-crUks_EP8vndnQ
CitedBy_id crossref_primary_10_1016_j_jlr_2024_100609
crossref_primary_10_1007_s10637_025_01546_3
crossref_primary_10_3389_fonc_2023_1230647
Cites_doi 10.1016/j.cell.2008.03.027
10.1038/s41598‐020‐63539‐x
10.1007/s10549‐012‐2315‐y
10.1038/s41588‐021‐00911‐1
10.1186/bcr2834
10.1200/JCO.19.02309
10.1002/cyto.b.20530
10.1016/j.cell.2018.08.039
10.1158/1078‐0432.CCR‐14‐0432
10.1634/theoncologist.2019‐0982
10.3390/cells11091538
10.1038/nrclinonc.2016.66
10.1002/cyto.a.22625
10.1073/pnas.1618298114
10.1093/nar/gkv468
10.1038/s41467‐019‐14134‐w
10.15252/embj.2019104063
10.7554/eLife.56879
10.1158/2159‐8290.CD‐19‐1384
10.3390/cancers13092188
10.1038/s41598‐019‐57094‐3
10.1158/2159‐8290.CD‐20‐1808
10.1016/j.cels.2018.02.010
10.15252/embj.2020107333
10.1016/j.ejca.2017.07.041
10.1038/ncomms15081
10.1038/s41392‐020‐0110‐5
10.1002/1878‐0261.12083
10.1186/s12885‐017‐3969‐y
10.1186/1476‐4598‐9‐179
10.1158/2159‐8290.CD‐18‐1177
10.1089/omi.2017.0020
10.1038/s41571‐021‐00565‐2
10.1038/s41467‐018‐06052‐0
10.1038/ncb3041
10.1038/s41467‐017‐01666‐2
10.1038/nm.3394
10.1016/j.cell.2019.03.005
10.1371/journal.pone.0182786
10.1016/j.clbc.2018.03.012
10.1016/j.semcancer.2019.08.004
10.1038/bjc.2017.497
10.1016/j.stem.2012.05.007
10.1371/journal.pone.0106966
10.1093/jnci/djaa201
10.1126/sciadv.aav4275
10.1101/2021.02.11.430721
10.1073/pnas.1812876116
ContentType Journal Article
Copyright 2022 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
2022 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2022 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
– notice: 2022 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
– notice: 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID 24P
AAYXX
CITATION
NPM
3V.
7X7
7XB
88E
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M7P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
7X8
5PM
DOA
DOI 10.1002/1878-0261.13365
DatabaseName Wiley Online Library Open Access
CrossRef
PubMed
ProQuest Central (Corporate)
Health & Medical Collection (ProQuest)
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central Database Suite (ProQuest)
Natural Science Collection
ProQuest One
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Collection (ProQuest)
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
ProQuest Health & Medical Collection
Medical Database
Biological Science Database (ProQuest)
Proquest Central Premium
ProQuest One Academic (New)
ProQuest Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic (retired)
ProQuest One Academic UKI Edition
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central Student
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Health & Medical Research Collection
Biological Science Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList
MEDLINE - Academic
Publicly Available Content Database
PubMed


CrossRef
Database_xml – sequence: 1
  dbid: 24P
  name: Wiley Online Library Open Access
  url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html
  sourceTypes: Publisher
– sequence: 2
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 3
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 4
  dbid: PIMPY
  name: Publicly Available Content Database
  url: http://search.proquest.com/publiccontent
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
DocumentTitleAlternate Single‐cell profiling of TNBC heterogeneity
EISSN 1878-0261
EndPage 1040
ExternalDocumentID oai_doaj_org_article_902a68d47202494e8964ad7e6e6c97dc
PMC10257414
36550781
10_1002_1878_0261_13365
MOL213365
Genre researchArticle
Journal Article
GeographicLocations United States--US
Germany
GeographicLocations_xml – name: United States--US
– name: Germany
GrantInformation_xml – fundername: Grantová Agentura České Republiky
  funderid: 20‐22984S; 21‐11585S
– fundername: Ministerstvo Školství, Mládeže a Tělovýchovy
  funderid: CZ.02.1.01/0.0/0.0/16_025/0007381
– fundername: Agentura Pro Zdravotnický Výzkum České Republiky
  funderid: NU21‐08‐00023; NV18‐08‐00245
– fundername: Agentura Pro Zdravotnický Výzkum České Republiky
  grantid: NU21-08-00023
– fundername: Grantová Agentura České Republiky
  grantid: 20-22984S
– fundername: Grantová Agentura České Republiky
  grantid: 21-11585S
– fundername: Ministerstvo Školství, Mládeže a Tělovýchovy
  grantid: CZ.02.1.01/0.0/0.0/16_025/0007381
– fundername: Agentura Pro Zdravotnický Výzkum České Republiky
  grantid: NV18-08-00245
– fundername: ;
  grantid: 20‐22984S; 21‐11585S
– fundername: ;
  grantid: CZ.02.1.01/0.0/0.0/16_025/0007381
– fundername: ;
  grantid: NU21‐08‐00023; NV18‐08‐00245
GroupedDBID ---
--K
.~1
0R~
0SF
123
1B1
1OC
1~.
24P
4.4
457
4G.
53G
5VS
6I.
7-5
71M
8FE
8FH
8P~
AACTN
AAEDW
AAFTH
AAFWJ
AAHHS
AAIKJ
AALRI
AAQFI
AAXUO
ABBQC
ABFRF
ABGSF
ABMAC
ABVKL
ABWVN
ACCFJ
ACCMX
ACGFO
ACGFS
ACRPL
ACXQS
ADBBV
ADEZE
ADMUD
ADNMO
ADPDF
ADUVX
ADVLN
AEEZP
AEFWE
AEKER
AENEX
AEQDE
AEXQZ
AFKRA
AFPKN
AGHFR
AGYEJ
AITUG
AIWBW
AJBDE
AJOXV
AJRQY
AKRWK
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMRAJ
AOIJS
AVUZU
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BLXMC
CCPQU
CS3
DIK
DU5
E3Z
EBS
EJD
EMOBN
EO9
EP2
EP3
F5P
FDB
FEDTE
FIRID
FNPLU
GBLVA
GROUPED_DOAJ
HCIFZ
HVGLF
HYE
HZ~
IAO
IHE
IHR
ITC
IXB
J1W
LK8
M41
M7P
MO0
N9A
NCXOZ
O-L
OAUVE
OK1
OVD
OVEED
OZT
P-8
P-9
P2P
PC.
PIMPY
PROAC
Q38
RIG
ROL
RPM
RPZ
SDF
SDG
SEL
SES
SSZ
TEORI
TR2
UNMZH
WIN
7X7
88E
8FI
8FJ
9DU
AAMMB
AAYWO
AAYXX
ABUWG
ACVFH
ADCNI
AEFGJ
AEUPX
AFFHD
AFPUW
AGXDD
AIDQK
AIDYY
AIGII
AKBMS
AKYEP
CITATION
EFLBG
FYUFA
HMCUK
M1P
PHGZM
PHGZT
PJZUB
PPXIY
PQGLB
PSQYO
UKHRP
~HD
NPM
3V.
7XB
8FK
AZQEC
DWQXO
GNUQQ
K9.
PKEHL
PQEST
PQQKQ
PQUKI
7X8
5PM
ID FETCH-LOGICAL-c5355-8decfd7be065dfb58b99e8ef7d3c164e6e7d90deb4bc66884f5b44214d148b373
IEDL.DBID 24P
ISICitedReferencesCount 3
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000926769000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1574-7891
1878-0261
IngestDate Fri Oct 03 12:51:31 EDT 2025
Tue Nov 04 02:06:50 EST 2025
Sun Nov 09 14:44:59 EST 2025
Tue Oct 07 06:46:20 EDT 2025
Thu Apr 03 07:05:18 EDT 2025
Sat Nov 29 05:53:46 EST 2025
Tue Nov 18 21:28:55 EST 2025
Wed Jan 22 16:22:36 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 6
Keywords unsupervised machine learning algorithm
triple-negative breast cancer
phenotypic plasticity
mass cytometry
single-cell profiles
tumor heterogeneity
Language English
License Attribution
2022 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c5355-8decfd7be065dfb58b99e8ef7d3c164e6e7d90deb4bc66884f5b44214d148b373
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0001-7283-8150
0000-0002-3228-2896
0000-0001-8086-9790
0000-0002-3547-5896
0000-0002-6478-9923
0000-0003-4842-1720
0000-0003-4475-2872
OpenAccessLink https://onlinelibrary.wiley.com/doi/abs/10.1002%2F1878-0261.13365
PMID 36550781
PQID 2824324969
PQPubID 4370298
PageCount 1040
ParticipantIDs doaj_primary_oai_doaj_org_article_902a68d47202494e8964ad7e6e6c97dc
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10257414
proquest_miscellaneous_2758109482
proquest_journals_2824324969
pubmed_primary_36550781
crossref_citationtrail_10_1002_1878_0261_13365
crossref_primary_10_1002_1878_0261_13365
wiley_primary_10_1002_1878_0261_13365_MOL213365
PublicationCentury 2000
PublicationDate June 2023
PublicationDateYYYYMMDD 2023-06-01
PublicationDate_xml – month: 06
  year: 2023
  text: June 2023
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Hoboken
PublicationTitle Molecular oncology
PublicationTitleAlternate Mol Oncol
PublicationYear 2023
Publisher John Wiley & Sons, Inc
John Wiley and Sons Inc
Wiley
Publisher_xml – name: John Wiley & Sons, Inc
– name: John Wiley and Sons Inc
– name: Wiley
References 2010; 78
2017; 84
2019; 9
2017; 8
2019; 5
2020; 62
2017; 21
2020; 39
2008; 9
2020; 38
2011; 13
2020; 11
2020; 10
2017; 114
2012; 10
2017; 9
2016; 13
2013; 19
2021; 13
2018; 6
2018; 174
2018; 9
2018; 18
2020; 5
2021; 53
2021; 11
2021; 113
2021
2018; 118
2017; 11
2021; 19
2020; 9
2015; 87
2015; 43
2015; 21
2017; 12
2014; 16
2019; 116
2020; 25
2022; 11
2012; 136
2014; 9
2008; 133
2021; 40
2019; 177
2010; 9
e_1_2_11_32_1
e_1_2_11_30_1
e_1_2_11_36_1
e_1_2_11_51_1
e_1_2_11_13_1
e_1_2_11_34_1
e_1_2_11_11_1
e_1_2_11_29_1
e_1_2_11_6_1
e_1_2_11_27_1
e_1_2_11_4_1
e_1_2_11_48_1
e_1_2_11_2_1
Tian H (e_1_2_11_42_1) 2017; 9
R Core Team (e_1_2_11_23_1) 2021
e_1_2_11_20_1
e_1_2_11_45_1
e_1_2_11_47_1
e_1_2_11_41_1
e_1_2_11_8_1
e_1_2_11_22_1
e_1_2_11_43_1
e_1_2_11_17_1
e_1_2_11_15_1
e_1_2_11_38_1
e_1_2_11_19_1
e_1_2_11_50_1
e_1_2_11_10_1
e_1_2_11_31_1
e_1_2_11_14_1
e_1_2_11_35_1
e_1_2_11_52_1
e_1_2_11_12_1
e_1_2_11_33_1
e_1_2_11_7_1
e_1_2_11_28_1
e_1_2_11_5_1
e_1_2_11_26_1
e_1_2_11_3_1
e_1_2_11_49_1
Van der Maaten L (e_1_2_11_24_1) 2008; 9
e_1_2_11_21_1
e_1_2_11_44_1
e_1_2_11_46_1
e_1_2_11_25_1
e_1_2_11_40_1
e_1_2_11_9_1
e_1_2_11_18_1
e_1_2_11_16_1
e_1_2_11_37_1
e_1_2_11_39_1
References_xml – volume: 174
  start-page: 1373
  year: 2018
  end-page: 87
  article-title: A structured tumor‐immune microenvironment in triple negative breast cancer revealed by multiplexed ion beam imaging
  publication-title: Cell
– volume: 11
  start-page: 933
  year: 2021
  end-page: 59
  article-title: Therapeutic targeting of the tumor microenvironment
  publication-title: Cancer Discov
– volume: 177
  start-page: 1330
  year: 2019
  end-page: 45
  article-title: A single‐cell atlas of the tumor and immune ecosystem of human breast cancer
  publication-title: Cell
– volume: 21
  start-page: 1688
  year: 2015
  end-page: 98
  article-title: Comprehensive genomic analysis identifies novel subtypes and targets of triple‐negative breast cancer
  publication-title: Clin Cancer Res
– volume: 16
  start-page: 1105
  year: 2014
  end-page: 17
  article-title: A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages
  publication-title: Nat Cell Biol
– year: 2021
– volume: 9
  start-page: 2579
  year: 2008
  end-page: 605
  article-title: Visualizing data using t‐SNE
  publication-title: J Mach Learn Res
– volume: 8
  start-page: 1669
  year: 2017
  article-title: Role of epithelial to mesenchymal transition associated genes in mammary gland regeneration and breast tumorigenesis
  publication-title: Nat Commun
– year: 2021
  article-title: A strategy to address dissociation‐induced compositional and transcriptional bias for single‐cell analysis of the human mammary gland
  publication-title: bioRxiv
– volume: 5
  start-page: 8
  year: 2020
  article-title: Targeting cancer stem cell pathways for cancer therapy
  publication-title: Signal Transduct Target Ther
– volume: 84
  start-page: 219
  year: 2017
  end-page: 27
  article-title: Interlaboratory variability of Ki67 staining in breast cancer
  publication-title: Eur J Cancer
– volume: 116
  start-page: 7353
  year: 2019
  end-page: 62
  article-title: Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells
  publication-title: Proc Natl Acad Sci USA
– volume: 10
  start-page: 225
  year: 2020
  article-title: The prognostic and predictive potential of Ki‐67 in triple‐negative breast cancer
  publication-title: Sci Rep
– volume: 18
  start-page: 56
  year: 2018
  article-title: Clinical and pathological factors influencing survival in a large cohort of triple‐negative breast cancer patients
  publication-title: BMC Cancer
– volume: 118
  start-page: 813
  year: 2018
  end-page: 9
  article-title: Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer
  publication-title: Br J Cancer
– volume: 21
  start-page: 225
  year: 2017
  end-page: 31
  article-title: NF‐kappaB is a potential molecular drug target in triple‐negative breast cancers
  publication-title: OMICS
– volume: 9
  start-page: 176
  year: 2019
  end-page: 98
  article-title: Insights into molecular classifications of triple‐negative breast cancer: improving patient selection for treatment
  publication-title: Cancer Discov
– volume: 43
  start-page: W566
  year: 2015
  end-page: 70
  article-title: ClustVis: a web tool for visualizing clustering of multivariate data using principal component analysis and heatmap
  publication-title: Nucleic Acids Res
– volume: 133
  start-page: 704
  year: 2008
  end-page: 15
  article-title: The epithelial‐mesenchymal transition generates cells with properties of stem cells
  publication-title: Cell
– volume: 38
  start-page: 1346
  year: 2020
  end-page: 66
  article-title: Estrogen and progesterone receptor testing in breast cancer: ASCO/CAP guideline update
  publication-title: J Clin Oncol
– volume: 11
  start-page: 404
  year: 2020
  article-title: Cancer‐associated fibroblast heterogeneity in axillary lymph nodes drives metastases in breast cancer through complementary mechanisms
  publication-title: Nat Commun
– volume: 19
  start-page: 1423
  year: 2013
  end-page: 37
  article-title: Microenvironmental regulation of tumor progression and metastasis
  publication-title: Nat Med
– volume: 13
  year: 2021
  article-title: Phenotypic heterogeneity of triple‐negative breast cancer mediated by epithelial‐mesenchymal plasticity
  publication-title: Cancers (Basel)
– volume: 113
  start-page: 808
  year: 2021
  end-page: 19
  article-title: Assessment of Ki67 in breast cancer: updated recommendations from the international Ki67 in breast cancer working group
  publication-title: J Natl Cancer Inst
– volume: 53
  start-page: 1334
  year: 2021
  end-page: 47
  article-title: A single‐cell and spatially resolved atlas of human breast cancers
  publication-title: Nat Genet
– volume: 8
  year: 2017
  article-title: Single‐cell RNA‐seq enables comprehensive tumour and immune cell profiling in primary breast cancer
  publication-title: Nat Commun
– volume: 19
  start-page: 91
  year: 2021
  end-page: 113
  article-title: Treatment landscape of triple‐negative breast cancer – expanded options, evolving needs
  publication-title: Nat Rev Clin Oncol
– volume: 10
  start-page: 1330
  year: 2020
  end-page: 51
  article-title: Single‐cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
  publication-title: Cancer Discov
– volume: 13
  start-page: R22
  year: 2011
  article-title: Ki‐67 can be used for further classification of triple negative breast cancer into two subtypes with different response and prognosis
  publication-title: Breast Cancer Res
– volume: 9
  start-page: 4640
  year: 2017
  end-page: 51
  article-title: Effects of targeted CD97 immune epitopes small interference RNA on cellular biological behaviors in MDA‐MB231 malignant breast cancer cell line
  publication-title: Am J Transl Res
– volume: 39
  year: 2020
  article-title: Stromal cell diversity associated with immune evasion in human triple‐negative breast cancer
  publication-title: EMBO J
– volume: 87
  start-page: 636
  year: 2015
  end-page: 45
  article-title: FlowSOM: using self‐organizing maps for visualization and interpretation of cytometry data
  publication-title: Cytometry A
– volume: 11
  year: 2022
  article-title: To detach, migrate, adhere, and metastasize: CD97/ADGRE5 in cancer
  publication-title: Cell
– volume: 12
  year: 2017
  article-title: Expression of the MHC class II in triple‐negative breast cancer is associated with tumor‐infiltrating lymphocytes and interferon signaling
  publication-title: PLoS One
– volume: 10
  start-page: 717
  year: 2012
  end-page: 28
  article-title: Cancer stem cells: current status and evolving complexities
  publication-title: Cell Stem Cell
– volume: 40
  year: 2021
  article-title: A single‐cell RNA expression atlas of normal, preneoplastic and tumorigenic states in the human breast
  publication-title: EMBO J
– volume: 114
  start-page: E2337
  year: 2017
  end-page: 46
  article-title: Integrin‐beta4 identifies cancer stem cell‐enriched populations of partially mesenchymal carcinoma cells
  publication-title: Proc Natl Acad Sci USA
– volume: 11
  start-page: 755
  year: 2017
  end-page: 69
  article-title: EMT and MET: necessary or permissive for metastasis?
  publication-title: Mol Oncol
– volume: 18
  start-page: e1123
  year: 2018
  end-page: 32
  article-title: Feasibility of classification of triple negative breast cancer by immunohistochemical surrogate markers
  publication-title: Clin Breast Cancer
– volume: 25
  start-page: e1481
  year: 2020
  end-page: 91
  article-title: Molecular subtyping of triple‐negative breast cancers by immunohistochemistry: molecular basis and clinical relevance
  publication-title: Oncologist
– volume: 5
  year: 2019
  article-title: Epithelial‐type systemic breast carcinoma cells with a restricted mesenchymal transition are a major source of metastasis
  publication-title: Sci Adv
– volume: 136
  start-page: 795
  year: 2012
  end-page: 804
  article-title: Clinical and biologic features of triple‐negative breast cancers in a large cohort of patients with long‐term follow‐up
  publication-title: Breast Cancer Res Treat
– volume: 9
  year: 2020
  article-title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
  publication-title: Elife
– volume: 78
  start-page: 287
  year: 2010
  end-page: 301
  article-title: Localization of CD44 and CD90 positive cells to the invasive front of breast tumors
  publication-title: Cytometry B Clin Cytom
– volume: 9
  year: 2014
  article-title: NF‐kappaB affects proliferation and invasiveness of breast cancer cells by regulating CD44 expression
  publication-title: PLoS One
– volume: 62
  start-page: 166
  year: 2020
  end-page: 81
  article-title: Cancer‐associated fibroblasts in desmoplastic tumors: emerging role of integrins
  publication-title: Semin Cancer Biol
– volume: 13
  start-page: 674
  year: 2016
  end-page: 90
  article-title: Triple‐negative breast cancer: challenges and opportunities of a heterogeneous disease
  publication-title: Nat Rev Clin Oncol
– volume: 9
  year: 2010
  article-title: Breast carcinoma cells re‐express E‐cadherin during mesenchymal to epithelial reverting transition
  publication-title: Mol Cancer
– volume: 9
  start-page: 3588
  year: 2018
  article-title: Unravelling subclonal heterogeneity and aggressive disease states in TNBC through single‐cell RNA‐seq
  publication-title: Nat Commun
– volume: 10
  start-page: 6598
  year: 2020
  article-title: Spatially‐resolved quantification of proteins in triple negative breast cancers reveals differences in the immune microenvironment associated with prognosis
  publication-title: Sci Rep
– volume: 6
  start-page: 612
  year: 2018
  end-page: 20
  article-title: Compensation of signal spillover in suspension and imaging mass cytometry
  publication-title: Cell Syst
– ident: e_1_2_11_7_1
  doi: 10.1016/j.cell.2008.03.027
– ident: e_1_2_11_44_1
  doi: 10.1038/s41598‐020‐63539‐x
– ident: e_1_2_11_3_1
  doi: 10.1007/s10549‐012‐2315‐y
– ident: e_1_2_11_38_1
  doi: 10.1038/s41588‐021‐00911‐1
– ident: e_1_2_11_32_1
  doi: 10.1186/bcr2834
– ident: e_1_2_11_27_1
  doi: 10.1200/JCO.19.02309
– ident: e_1_2_11_36_1
  doi: 10.1002/cyto.b.20530
– ident: e_1_2_11_37_1
  doi: 10.1016/j.cell.2018.08.039
– ident: e_1_2_11_28_1
  doi: 10.1158/1078‐0432.CCR‐14‐0432
– ident: e_1_2_11_30_1
  doi: 10.1634/theoncologist.2019‐0982
– ident: e_1_2_11_41_1
  doi: 10.3390/cells11091538
– ident: e_1_2_11_2_1
  doi: 10.1038/nrclinonc.2016.66
– ident: e_1_2_11_25_1
  doi: 10.1002/cyto.a.22625
– ident: e_1_2_11_11_1
  doi: 10.1073/pnas.1618298114
– ident: e_1_2_11_26_1
  doi: 10.1093/nar/gkv468
– volume: 9
  start-page: 4640
  year: 2017
  ident: e_1_2_11_42_1
  article-title: Effects of targeted CD97 immune epitopes small interference RNA on cellular biological behaviors in MDA‐MB231 malignant breast cancer cell line
  publication-title: Am J Transl Res
– ident: e_1_2_11_50_1
  doi: 10.1038/s41467‐019‐14134‐w
– ident: e_1_2_11_18_1
  doi: 10.15252/embj.2019104063
– ident: e_1_2_11_22_1
  doi: 10.7554/eLife.56879
– ident: e_1_2_11_49_1
  doi: 10.1158/2159‐8290.CD‐19‐1384
– ident: e_1_2_11_6_1
  doi: 10.3390/cancers13092188
– ident: e_1_2_11_34_1
  doi: 10.1038/s41598‐019‐57094‐3
– ident: e_1_2_11_15_1
  doi: 10.1158/2159‐8290.CD‐20‐1808
– ident: e_1_2_11_21_1
  doi: 10.1016/j.cels.2018.02.010
– ident: e_1_2_11_17_1
  doi: 10.15252/embj.2020107333
– ident: e_1_2_11_39_1
  doi: 10.1016/j.ejca.2017.07.041
– ident: e_1_2_11_19_1
  doi: 10.1038/ncomms15081
– ident: e_1_2_11_13_1
  doi: 10.1038/s41392‐020‐0110‐5
– ident: e_1_2_11_9_1
  doi: 10.1002/1878‐0261.12083
– ident: e_1_2_11_33_1
  doi: 10.1186/s12885‐017‐3969‐y
– ident: e_1_2_11_10_1
  doi: 10.1186/1476‐4598‐9‐179
– ident: e_1_2_11_5_1
  doi: 10.1158/2159‐8290.CD‐18‐1177
– ident: e_1_2_11_45_1
  doi: 10.1089/omi.2017.0020
– ident: e_1_2_11_4_1
  doi: 10.1038/s41571‐021‐00565‐2
– volume: 9
  start-page: 2579
  year: 2008
  ident: e_1_2_11_24_1
  article-title: Visualizing data using t‐SNE
  publication-title: J Mach Learn Res
– ident: e_1_2_11_16_1
  doi: 10.1038/s41467‐018‐06052‐0
– ident: e_1_2_11_35_1
  doi: 10.1038/ncb3041
– ident: e_1_2_11_48_1
  doi: 10.1038/s41467‐017‐01666‐2
– ident: e_1_2_11_14_1
  doi: 10.1038/nm.3394
– ident: e_1_2_11_20_1
  doi: 10.1016/j.cell.2019.03.005
– ident: e_1_2_11_43_1
  doi: 10.1371/journal.pone.0182786
– ident: e_1_2_11_29_1
  doi: 10.1016/j.clbc.2018.03.012
– ident: e_1_2_11_51_1
  doi: 10.1016/j.semcancer.2019.08.004
– ident: e_1_2_11_31_1
  doi: 10.1038/bjc.2017.497
– ident: e_1_2_11_8_1
  doi: 10.1016/j.stem.2012.05.007
– ident: e_1_2_11_46_1
  doi: 10.1371/journal.pone.0106966
– ident: e_1_2_11_40_1
  doi: 10.1093/jnci/djaa201
– ident: e_1_2_11_47_1
  doi: 10.1126/sciadv.aav4275
– ident: e_1_2_11_52_1
  doi: 10.1101/2021.02.11.430721
– volume-title: R: a language and environment for statistical computing
  year: 2021
  ident: e_1_2_11_23_1
– ident: e_1_2_11_12_1
  doi: 10.1073/pnas.1812876116
SSID ssj0056969
Score 2.3720222
Snippet Triple‐negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic,...
Triple-negative breast cancer (TNBC) is an aggressive and complex subtype of breast cancer that lacks targeted therapy. TNBC manifests characteristic,...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
wiley
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 1024
SubjectTerms Antibodies
Bar codes
Breast Cancer
Cells
Cloning
Cytometry
Fibroblasts
Glycerol
mass cytometry
Metastasis
Patients
phenotypic plasticity
Proteins
Proteomics
Single-cell protein
single‐cell profiles
Stromal cells
Surgery
triple‐negative breast cancer
Tumor cells
tumor heterogeneity
Tumor microenvironment
Tumors
unsupervised machine learning algorithm
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQhRAXxJvQgozEgUu6cez4caQVFQdakACpNyt-lZWqtNrdwpWfwG_sL2HGzka7AtQLt2jHG1kzn-35nNE3hLzuWTLYTLKW0qla-IbXLnV9HbgRykQTvEm52YQ6OdGnp-bTRqsvrAkr8sDFcTPTtL3UQagWxe1E1EaKPqgoo_RGBY-7b6PMmkyVPbiTJjezYx1WG2rD1qI-TTtjGjVlgTjsAz3DM2XjPMqy_X_LNf8smdxMZfNZdHSf3BuTSPq2TP4BuRWHh-TO8fiZ_BH58RkOpPN4_fMX3svTrMUwH2jpzw0mGmLCcndazFMPryXtx2jFQPGGlq4WeBEPLxriWZYIpw6r2FfUI1oWtD_LfH3cMh-Tr0fvvhy-r8cOC7XvINGodYg-BeUiJCIhuU47Y6KOSQXugUeBk1UwTYhOOC-l1iJ1ToiWiQAsynHFn5Cd4WKIzwhlLsm-5ZwbHiBJ4M6IJibmesFQMEdVZH_tZ-tH-XHsgnFui3ByazEwFgNjc2Aq8mb6w2VR3vj30AMM3DQMJbPzDwAkOwLJ3gSkiuytw27Hdby0QEhRshDQVJFXkxlWIManH-LFFYwBysWAJeu2Ik8LSqaZwOQ6lFOqiN7Cz9ZUty3D_FtW-YbMD9DLREVmGWo3OcEef_zQ5qfn_8Mdu-QuWHipitsjO6vFVXxBbvvvq_ly8TKvtt-4iSy0
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Health & Medical Collection (ProQuest)
  dbid: 7X7
  link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB5BQYgLb2igICNx4OLuJnb8OCFAVBxoQQKkvVnxI8tKVbZNtvx-PI43dMXrwC2KHcnOjO35xqPvA3jRlK1GMUkqhJWUuzmjtq0b6pnmUgftnW6T2IQ8OVGLhf6UE25DLqvc7olpo_ZrhznyWYQGSB6nhX51dk5RNQpvV7OExlW4hrLZ6OdyMQGuWugkaVfWWHOodLml9plXs9hbUcQfhxGk4cly6VRK5P2_izh_LZy8HNCmE-no9v_O5Q7cyrEoeT06z124Erp7cOM437bfh-FzPNdOA8XkPkmEDquOjCLfsYH40GLNPBmbJyGwgTTZ5METTPOSTY_ZfNqFZWIZJxYL4TfEocP1pFkmyJ933Qfw9ejdl7fvaRZpoK6OsQpVPrjWSxtiLONbWyurdVChlZ65CMWCCNLruQ-WWyeEUrytLedVyX0EYpZJ9hD2unUX9oGUthVNxRjTzMc4g1nN56EtbcNL5NyRBRxujWRcZjBHIY1TM3IvVwatatCqJlm1gJfTB2cjecefu75Bq0_dkHU7vVj3S5MXsdHzqhHKc1kh0SIPSgveeBnnKJyW3hVwsLW3yVvBYH4au4DnU3NcxGidpgvri9gnorYyAm1VFfBodLFpJHFwNTIyFaB2nG9nqLst3epbIgqPwWN0_ZIXMEt--q-fYI4_fqjS0-O_z-QJ3Iy_gI0lcwewt-kvwlO47r5vVkP_LC3CHzaON7A
  priority: 102
  providerName: ProQuest
Title Single‐cell protein profiling defines cell populations associated with triple‐negative breast cancer aggressiveness
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2F1878-0261.13365
https://www.ncbi.nlm.nih.gov/pubmed/36550781
https://www.proquest.com/docview/2824324969
https://www.proquest.com/docview/2758109482
https://pubmed.ncbi.nlm.nih.gov/PMC10257414
https://doaj.org/article/902a68d47202494e8964ad7e6e6c97dc
Volume 17
WOSCitedRecordID wos000926769000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: DOA
  dateStart: 20170101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVPQU
  databaseName: Biological Science Database
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: M7P
  dateStart: 20070601
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/biologicalscijournals
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Health & Medical Collection
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: 7X7
  dateStart: 20070601
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/healthcomplete
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: ProQuest Central
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: BENPR
  dateStart: 20070601
  isFulltext: true
  titleUrlDefault: https://www.proquest.com/central
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: Publicly Available Content Database
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: PIMPY
  dateStart: 20070601
  isFulltext: true
  titleUrlDefault: http://search.proquest.com/publiccontent
  providerName: ProQuest
– providerCode: PRVWIB
  databaseName: Wiley Online Library Free Content
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: WIN
  dateStart: 20070101
  isFulltext: true
  titleUrlDefault: https://onlinelibrary.wiley.com
  providerName: Wiley-Blackwell
– providerCode: PRVWIB
  databaseName: Wiley Online Library Open Access
  customDbUrl:
  eissn: 1878-0261
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0056969
  issn: 1574-7891
  databaseCode: 24P
  dateStart: 20070101
  isFulltext: true
  titleUrlDefault: https://authorservices.wiley.com/open-science/open-access/browse-journals.html
  providerName: Wiley-Blackwell
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lj9MwELZgixAX3guBpTISBy7ZxrHjx5FFu2IlWioeopyi-JFSaZWitgtXfgK_kV_CjJMGykMIiUuUZBzLGc_YM5PJN4Q8qlhtsJhkKqVVqXAZT21dVKnnRigTjHemjsUm1GSiZzMz7bIJ8V-YFh-iD7ihZsT1GhW8suvRd9BQphEbFhyAQ3CzZHGRDBjjCgU7F9PtYlxIE6vasQLTDrVhW3SfLB_91MHOxhTx-39ndP6aO_mjTRs3pZNr_-F1rpOrnUVKn7QidINcCM1NcnncfXO_RT69gt3tLHz9_AWD_DQCOywa2hb7BhL1ocbcedqS-4Jga1p1Ux88xXAv3awwqg8dNWEe8capxZT4DXUoeitazaPz362_t8mbk-PXT5-lXbmG1BVgtaTaB1d7ZQNYNb62hbbGBB1q5bkDpyzIoLzJfLDCOim1FnVhhciZ8OCSWa74Ptlrlk24SyiztaxyzrnhHiwObo3IQs1sJRii76iEHG7nqnQdljmW1DgrWxTmvER2lsjOMrIzIY_7Bz60MB5_bnqEk983Q_zteGO5mpedOpcmyyupvVA5Qi6KoI0UlVfwjtIZ5V1CDraiU3aLwroE7xbxD0EiE_KwJ4M64_xUTVieQxvw3xi43DpPyJ1W0vqRwOAKxGZKiN6RwZ2h7lKaxfsIGQ5mJGgAEwkZRSH8GxPK8YvneTy7989P3CdXgCu8zac7IHub1Xl4QC65j5vFejWM6glHNVPxqIdkcHQ8mb4cxnDIEJNvgT6Yno6n7-Dq7enkG9BWQqg
linkProvider Wiley-Blackwell
linkToHtml http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFH4qUwRc2JdAASOBxCWdJHa8HBBiqzrqzFCJIpVTiJcMI1WZMjMF8af4jfg5ydAR26kHblHsRLbzvc1--R7A4zKtFBaTjDnXImYmobGu8jK2VDGhnLJGVaHYhBiP5eGh2t-A792_MJhW2enEoKjtzOAeed-HBkgep7h6fvw5xqpReLraldBoYLHnvn31Idvi2eC1_75PsmznzcGr3bitKhCb3BvXWFpnKiu088bXVjqXWiknXSUsNT52cNwJqxLrNNOGcylZlWvGspRZHzloKqh_7znY9LfypAeb-4PR_odO9-dchSJ6aY5ZjlKlHZlQkvVTiVy2PmDZ9mEh2rJTdjCUC_idj_trquZpFzrYwJ0r_9vqXYXLrbdNXjTicQ02XH0dLozafIIbsHjnLfeRi_H4ggTKimlNmjLmvoFYV-FfAaRpXpU6W5CyBbWzBDeyyXKO5xVx7SaBR51oTPVfEoMiNSflJGxqtHblJrw_kynfgl49q90dIKmueJlRShW13pOiWrHEVakuWYqsQiKC7Q4UhWk52rFUyFHRsEtnBaKoQBQVAUURPF09cNzQk_y560tE2aob8oqHG7P5pGjVVKGSrOTSMpEhlSRzUnFWWuHnyI0S1kSw1eGraJXdovgJrggerZq9msKvU9ZuduL7-Lg0TRSTWQS3G0ivRuIHlyPnVARyDexrQ11vqaefAhW6d4-9qKUsgn6Qi38tQjF6O8zC1d2_z-QhXNw9GA2L4WC8dw8u-eWgTYLgFvSW8xN3H86bL8vpYv6gVQEEPp613PwAQMyXzA
linkToPdf http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1bb9MwFD4aHZp44X4JDDASSLxkTWInth8QYmwV07ZScZH2lsW3UmlKR9uB-Gv8OnycpKzi9rQH3qLajWznOzf7-DsAT6vUSSwmGReF4jHTCY2Vy6vYUMm4tNJo6UKxCT4ciqMjOVqD791dGEyr7HRiUNRmqnGPvO9DAySPk4XsuzYtYrQzeHn6OcYKUnjS2pXTaCCyb7999eHb_MXejv_Wz7JssPvh9Zu4rTAQ69wb2lgYq53hynpDbJzKhZLSCuu4odrHEbaw3MjEWMWULgohmMsVY1nKjI8iFOXUv_cSrHPqg54erG_vDkfvOjuQFzIU1EtzzHgUMu2IhZKsnwrktfXBy5YPEdGunbOJoXTA7_zdX9M2z7vTwR4Orv3PK3kdrrZeOHnViM0NWLP1Tdg4bPMMbsH8vbfoJzbGYw0SqCwmNWnKm_sGYqzD2wKkaV6WQJuTqgW7NQQ3uMlihucYcW3HgV-dKLwCsCAaRW1GqnHY7GjtzW34eCFTvgO9elrbe0BS5Yoqo5RKaryHRZVkiXWpqliKbEM8gq0OIKVuuduxhMhJ2bBOZyUiqkRElQFRETxf_uG0oS35c9dtRNyyG_KNhx-ms3HZqq9SJllVCMN4hhSTzApZsMpwP8dCS250BJsd1spWCc7Ln0CL4Mmy2asv_DpVbadnvo-PV9NEMpFFcLeB93IkfnA5clFFIFaAvzLU1ZZ68ilQpHu32YtdyiLoBxn51yKUh28PsvB0_-8zeQwbXljKg73h_gO44leDNnmDm9BbzM7sQ7isvywm89mjVhsQOL5osfkBG5egZg
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Single%E2%80%90cell+protein+profiling+defines+cell+populations+associated+with+triple%E2%80%90negative+breast+cancer+aggressiveness&rft.jtitle=Molecular+oncology&rft.au=Kvoka%C4%8Dkov%C3%A1%2C+Barbora&rft.au=Fedr%2C+Radek&rft.au=Ku%C5%BE%C3%ADlkov%C3%A1%2C+Daniela&rft.au=Stuchl%C3%BD%2C+Jan&rft.date=2023-06-01&rft.pub=John+Wiley+and+Sons+Inc&rft.issn=1574-7891&rft.eissn=1878-0261&rft.volume=17&rft.issue=6&rft.spage=1024&rft.epage=1040&rft_id=info:doi/10.1002%2F1878-0261.13365&rft_id=info%3Apmid%2F36550781&rft.externalDocID=PMC10257414
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1574-7891&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1574-7891&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1574-7891&client=summon