High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics

•Column length was found as an important factor for top-down proteomic RPLC separation.•Long (≥1m) columns can provide peak capacities of >400 for resolving proteoforms.•Both porous and superficially porous particles were effective to separate proteins. Particles with 200–450Å pores enabled chrom...

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Bibliographic Details
Published in:	Journal of Chromatography Vol. 1498; no. C; pp. 99 - 110
Main Authors:	Shen, Yufeng, Tolić, Nikola, Piehowski, Paul D., Shukla, Anil K., Kim, Sangtae, Zhao, Rui, Qu, Yi, Robinson, Errol, Smith, Richard D., Paša-Tolić, Ljiljana
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 19.05.2017 Elsevier
Subjects:	BASIC BIOLOGICAL SCIENCES Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Columns and stationary phases INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY Intact proteins Mass spectrometry molecular weight peptides Peptides - analysis Peptides - isolation & purification Porosity Pressure proteins Proteins - analysis Proteins - isolation & purification Proteoforms proteomics Proteomics - methods reversed-phase liquid chromatography spectrometers Surface Properties Tandem Mass Spectrometry Top-down proteomics UPLC Top-down proteomics Columns and stationary phases Intact proteins UPLC Mass spectrometry Proteoforms
ISSN:	0021-9673, 1873-3778, 1873-3778
Online Access:	Get full text
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