Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy

We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accura...

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Vydáno v:Journal of biomedical optics Ročník 17; číslo 12; s. 126008
Hlavní autoři: Dedecker, Peter, Duwé, Sam, Neely, Robert K, Zhang, Jin
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.12.2012
Témata:
Algorithms
Image Enhancement - methods
Image Interpretation, Computer-Assisted - methods
Microscopy, Fluorescence - methods
Programming Languages
Reproducibility of Results
Sensitivity and Specificity
Software
Software Validation
ISSN:1560-2281, 1560-2281
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Popis
Shrnutí:We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accuracy and performance and comes with a fully featured and easy-to-use graphical user interface but is also designed to be integrated in higher-level analysis environments. Due to its modular design, Localizer can be readily extended with new algorithms as they become available, while maintaining the same interface and performance. We provide front-ends for running Localizer from Igor Pro, Matlab, or as a stand-alone program. We show that Localizer performs favorably when compared with two existing superresolution packages, and to our knowledge is the only freely available implementation of SOFI/pcSOFI microscopy. By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies.
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ISSN:1560-2281
1560-2281
DOI:10.1117/1.JBO.17.12.126008