A plant virus vector for systemic expression of foreign genes in cereals

Summary Inserts bearing the coding sequences of NPT II and β‐glucuronidase (GUS) were placed between the nuclear inclusion b (NIb) and coat protein (CP) domains of the wheat streak mosaic virus (WSMV) polyprotein ORF. The WSMV NIb‐CP junction containing the nuclear inclusion a (NIa) protease cleavag...

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Bibliographic Details
Published in:	The Plant journal : for cell and molecular biology Vol. 23; no. 4; pp. 547 - 555
Main Authors:	Choi, Il‐Ryong, Stenger, Drake C., Morris, T. Jack, French, Roy
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01.08.2000 Blackwell Science
Subjects:	Agronomy. Soil science and plant productions Avena sativa beta-glucuronidase Biological and medical sciences Biotechnology Carrier Proteins Carrier Proteins - genetics Carrier Proteins - metabolism cereals coat proteins Edible Grain Edible Grain - genetics Edible Grain - metabolism Edible Grain - virology Fundamental and applied biological sciences. Psychology gene expression Gene Transfer Techniques Genes, Reporter Genetic engineering Genetic engineering applications Genetic technics genetic transformation Genetic Vectors genetics Genetics and breeding of economic plants Glucuronidase Glucuronidase - genetics Glucuronidase - metabolism GUS gene histochemistry Hordeum vulgare infection interspecific variation leaves metabolism Methods. Procedures. Technologies monocotyledonous plants Mosaic Viruses Mosaic Viruses - genetics NIb protein NPT II gene nptII gene open reading frames Plant breeding: fundamental aspects and methodology Plant Roots Plant Roots - metabolism polymerase chain reaction reporter genes Reverse Transcriptase Polymerase Chain Reaction roots seedlings Sodium-Phosphate Cotransporter Proteins Symporters Transgenic animals and transgenic plants Transgenic plants Triticum aestivum virology virulence virus gene expression vector Wheat streak mosaic virus Zea mays Monocotyledones β-Glucuronidase Genetic transformation Zea mays Plant pathogen Enzyme Plant juvenile growth stage Insertion Gene expression Cereal crop Virus Gramineae Glycosidases Angiospermae Cereal Hydrolases Spermatophyta Genetic engineering Transgenic plant O-Glycosidases Vector Triticum aestivum
ISSN:	0960-7412, 1365-313X
Online Access:	Get full text
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