Identification and characterization of enhancers controlling the inflammatory gene expression program in macrophages
Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the acetyl-transferase p300, which has allowed genome-wide enhancer identification. However, the regulatory principles by which subsets of enhancers become...
Uloženo v:
| Vydáno v: | Immunity (Cambridge, Mass.) Ročník 32; číslo 3; s. 317 |
|---|---|
| Hlavní autoři: | , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
26.03.2010
|
| Témata: | |
| ISSN: | 1097-4180, 1097-4180 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the acetyl-transferase p300, which has allowed genome-wide enhancer identification. However, the regulatory principles by which subsets of enhancers become active in specific developmental and/or environmental contexts are unknown. We exploited inducible p300 binding to chromatin to identify, and then mechanistically dissect, enhancers controlling endotoxin-stimulated gene expression in macrophages. In these enhancers, binding sites for the lineage-restricted and constitutive Ets protein PU.1 coexisted with those for ubiquitous stress-inducible transcription factors such as NF-kappaB, IRF, and AP-1. PU.1 was required for maintaining H3K4me1 at macrophage-specific enhancers. Reciprocally, ectopic expression of PU.1 reactivated these enhancers in fibroblasts. Thus, the combinatorial assembly of tissue- and signal-specific transcription factors determines the activity of a distinct group of enhancers. We suggest that this may represent a general paradigm in tissue-restricted and stimulus-responsive gene regulation. |
|---|---|
| AbstractList | Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the acetyl-transferase p300, which has allowed genome-wide enhancer identification. However, the regulatory principles by which subsets of enhancers become active in specific developmental and/or environmental contexts are unknown. We exploited inducible p300 binding to chromatin to identify, and then mechanistically dissect, enhancers controlling endotoxin-stimulated gene expression in macrophages. In these enhancers, binding sites for the lineage-restricted and constitutive Ets protein PU.1 coexisted with those for ubiquitous stress-inducible transcription factors such as NF-kappaB, IRF, and AP-1. PU.1 was required for maintaining H3K4me1 at macrophage-specific enhancers. Reciprocally, ectopic expression of PU.1 reactivated these enhancers in fibroblasts. Thus, the combinatorial assembly of tissue- and signal-specific transcription factors determines the activity of a distinct group of enhancers. We suggest that this may represent a general paradigm in tissue-restricted and stimulus-responsive gene regulation.Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the acetyl-transferase p300, which has allowed genome-wide enhancer identification. However, the regulatory principles by which subsets of enhancers become active in specific developmental and/or environmental contexts are unknown. We exploited inducible p300 binding to chromatin to identify, and then mechanistically dissect, enhancers controlling endotoxin-stimulated gene expression in macrophages. In these enhancers, binding sites for the lineage-restricted and constitutive Ets protein PU.1 coexisted with those for ubiquitous stress-inducible transcription factors such as NF-kappaB, IRF, and AP-1. PU.1 was required for maintaining H3K4me1 at macrophage-specific enhancers. Reciprocally, ectopic expression of PU.1 reactivated these enhancers in fibroblasts. Thus, the combinatorial assembly of tissue- and signal-specific transcription factors determines the activity of a distinct group of enhancers. We suggest that this may represent a general paradigm in tissue-restricted and stimulus-responsive gene regulation. Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the acetyl-transferase p300, which has allowed genome-wide enhancer identification. However, the regulatory principles by which subsets of enhancers become active in specific developmental and/or environmental contexts are unknown. We exploited inducible p300 binding to chromatin to identify, and then mechanistically dissect, enhancers controlling endotoxin-stimulated gene expression in macrophages. In these enhancers, binding sites for the lineage-restricted and constitutive Ets protein PU.1 coexisted with those for ubiquitous stress-inducible transcription factors such as NF-kappaB, IRF, and AP-1. PU.1 was required for maintaining H3K4me1 at macrophage-specific enhancers. Reciprocally, ectopic expression of PU.1 reactivated these enhancers in fibroblasts. Thus, the combinatorial assembly of tissue- and signal-specific transcription factors determines the activity of a distinct group of enhancers. We suggest that this may represent a general paradigm in tissue-restricted and stimulus-responsive gene regulation. |
| Author | Barozzi, Iros Gregory, Lorna Chew, Adeline Ghisletti, Serena De Santa, Francesca Lonie, Lorne Wei, Chia-Lin Mietton, Flore Polletti, Sara Ragoussis, Jiannis Venturini, Elisa Natoli, Gioacchino |
| Author_xml | – sequence: 1 givenname: Serena surname: Ghisletti fullname: Ghisletti, Serena organization: Department of Experimental Oncology, European Institute of Oncology (IEO), IFOM-IEO Campus, Via Adamello 16, Milan, Italy – sequence: 2 givenname: Iros surname: Barozzi fullname: Barozzi, Iros – sequence: 3 givenname: Flore surname: Mietton fullname: Mietton, Flore – sequence: 4 givenname: Sara surname: Polletti fullname: Polletti, Sara – sequence: 5 givenname: Francesca surname: De Santa fullname: De Santa, Francesca – sequence: 6 givenname: Elisa surname: Venturini fullname: Venturini, Elisa – sequence: 7 givenname: Lorna surname: Gregory fullname: Gregory, Lorna – sequence: 8 givenname: Lorne surname: Lonie fullname: Lonie, Lorne – sequence: 9 givenname: Adeline surname: Chew fullname: Chew, Adeline – sequence: 10 givenname: Chia-Lin surname: Wei fullname: Wei, Chia-Lin – sequence: 11 givenname: Jiannis surname: Ragoussis fullname: Ragoussis, Jiannis – sequence: 12 givenname: Gioacchino surname: Natoli fullname: Natoli, Gioacchino |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20206554$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkMtKQzEQhoNUrK2-gUh2rlpzOTmXpRQvhYIbXZdpMmlTTpKapKA-vZVWcDXDx_cPwz8igxADEnLD2ZQzXt9vp877fXBTwQ6IiSlj7Rm55KxrJhVv2eDfPiSjnLeM8Up17IIMBROsVqq6JGVuMBRnnYbiYqAQDNUbSKALJvd9hNFSDBsIGlOmOoaSYt-7sKZlg9QF24P3UGL6omsMSPFzlzDn3-QuxXUCf5CoB53ibgNrzFfk3EKf8fo0x-T96fFt9jJZvD7PZw-LiVZSlkndNFa22FVGKVEzlFq1HefCSGFrAwJ0ZbRUjQHT1aIy1tYIApXUnUC70mJM7o53D2987DGXpXdZY99DwLjPy0bKVraCVwfz9mTuVx7Ncpech_S1_CtK_AA8BnHd |
| CitedBy_id | crossref_primary_10_1146_annurev_genet_110711_155459 crossref_primary_10_1016_j_molcel_2014_03_038 crossref_primary_10_1186_s13040_015_0071_3 crossref_primary_10_1016_j_exphem_2011_02_009 crossref_primary_10_1016_j_smim_2016_04_002 crossref_primary_10_1038_s41467_019_11053_8 crossref_primary_10_1016_j_it_2019_12_002 crossref_primary_10_3389_fimmu_2022_986589 crossref_primary_10_1016_j_molcel_2015_04_006 crossref_primary_10_3389_fimmu_2022_872695 crossref_primary_10_1002_biot_202400455 crossref_primary_10_1080_21645515_2022_2040238 crossref_primary_10_1016_j_molmed_2021_09_004 crossref_primary_10_3390_ijms21228462 crossref_primary_10_1016_j_ccell_2024_04_002 crossref_primary_10_1186_s12864_015_1704_0 crossref_primary_10_1186_1471_2164_14_92 crossref_primary_10_1016_j_cytogfr_2020_03_003 crossref_primary_10_1089_ars_2016_6695 crossref_primary_10_1016_j_smim_2015_02_004 crossref_primary_10_1371_journal_pcbi_1003677 crossref_primary_10_1073_pnas_2005924117 crossref_primary_10_1016_j_immuni_2010_03_011 crossref_primary_10_1016_j_biocel_2015_04_020 crossref_primary_10_1038_ni_2070 crossref_primary_10_1101_gr_192005_115 crossref_primary_10_1016_j_cell_2012_11_007 crossref_primary_10_3390_pathogens1010037 crossref_primary_10_1186_s12964_023_01206_0 crossref_primary_10_1093_nar_gky491 crossref_primary_10_1038_s41586_024_07662_z crossref_primary_10_1371_journal_pbio_1000384 crossref_primary_10_1016_j_immuni_2017_08_002 crossref_primary_10_1242_dev_120048 crossref_primary_10_1016_j_lfs_2023_121574 crossref_primary_10_1016_j_immuni_2013_08_009 crossref_primary_10_1128_MCB_00920_10 crossref_primary_10_1016_j_molcel_2012_07_030 crossref_primary_10_1186_s13072_019_0279_4 crossref_primary_10_1016_j_lungcan_2012_10_003 crossref_primary_10_1038_ni0710_572 crossref_primary_10_1016_j_pneurobio_2020_101971 crossref_primary_10_1016_j_atherosclerosis_2018_10_005 crossref_primary_10_1016_j_bbi_2020_05_058 crossref_primary_10_1182_blood_2012_06_437863 crossref_primary_10_1371_journal_pgen_1001392 crossref_primary_10_1074_jbc_RA119_010149 crossref_primary_10_1002_biot_202400474 crossref_primary_10_3390_cells10040753 crossref_primary_10_3389_fimmu_2024_1452410 crossref_primary_10_1038_nbt_3652 crossref_primary_10_1007_s13273_019_00057_6 crossref_primary_10_1016_j_cell_2011_01_024 crossref_primary_10_1038_s41467_020_20765_1 crossref_primary_10_1002_eji_201546035 crossref_primary_10_1128_MCB_00027_15 crossref_primary_10_1134_S1022795415100099 crossref_primary_10_1038_ni0710_565 crossref_primary_10_1074_jbc_M116_734186 crossref_primary_10_1016_j_tem_2013_09_006 crossref_primary_10_4049_jimmunol_1700223 crossref_primary_10_1016_j_immuni_2018_11_010 crossref_primary_10_1111_imr_12955 crossref_primary_10_1074_jbc_M112_413260 crossref_primary_10_1038_nprot_2014_062 crossref_primary_10_1038_ncomms12254 crossref_primary_10_4161_trns_2_6_18747 crossref_primary_10_1038_srep46440 crossref_primary_10_1101_gad_349282_121 crossref_primary_10_1146_annurev_cancerbio_060524_105603 crossref_primary_10_3389_fcell_2022_931493 crossref_primary_10_1038_nri3149 crossref_primary_10_1038_emboj_2011_295 crossref_primary_10_1007_s12185_015_1762_8 crossref_primary_10_1016_j_jmb_2014_08_015 crossref_primary_10_1016_j_biocel_2018_03_012 crossref_primary_10_1016_j_molimm_2020_10_011 crossref_primary_10_1038_ncomms15656 crossref_primary_10_1016_j_molcel_2013_07_010 crossref_primary_10_3389_fmicb_2022_991678 crossref_primary_10_1016_j_molcel_2012_05_011 crossref_primary_10_1111_j_1600_065X_2011_01091_x crossref_primary_10_1016_j_smim_2012_11_002 crossref_primary_10_1016_j_imbio_2011_07_025 crossref_primary_10_1016_j_jbc_2024_107771 crossref_primary_10_3389_fmed_2022_1085339 crossref_primary_10_1101_gr_233346_117 crossref_primary_10_1097_PR9_0000000000000892 crossref_primary_10_1172_JCI83585 crossref_primary_10_1038_nature14154 crossref_primary_10_3389_fimmu_2022_1026173 crossref_primary_10_3389_fimmu_2022_825032 crossref_primary_10_1093_nar_gkz020 crossref_primary_10_1016_j_molcel_2013_07_022 crossref_primary_10_1134_S1068162015060084 crossref_primary_10_1517_14712598_2015_1029452 crossref_primary_10_1016_j_immuni_2024_02_004 crossref_primary_10_1016_j_neuint_2025_106005 crossref_primary_10_1038_s41577_020_0285_6 crossref_primary_10_1111_imr_12647 crossref_primary_10_3389_fimmu_2022_849451 crossref_primary_10_1038_nrm3949 crossref_primary_10_1146_annurev_immunol_101320_031555 crossref_primary_10_1371_journal_pone_0141470 crossref_primary_10_1093_nar_gku1018 crossref_primary_10_1016_j_bbagrm_2019_194443 crossref_primary_10_1093_nar_gkac613 crossref_primary_10_1093_nar_gkt826 crossref_primary_10_1038_s41583_023_00709_6 crossref_primary_10_1038_s41467_021_26864_x crossref_primary_10_1021_cr2001355 crossref_primary_10_1186_s13059_018_1524_z crossref_primary_10_1016_j_tcb_2024_03_006 crossref_primary_10_4049_jimmunol_1601781 crossref_primary_10_1371_journal_pone_0184850 crossref_primary_10_1038_s41590_019_0453_7 crossref_primary_10_1186_s13072_016_0065_5 crossref_primary_10_1038_ni_3325 crossref_primary_10_1038_ni_3324 crossref_primary_10_1073_pnas_1017214108 crossref_primary_10_1016_j_immuni_2019_11_009 crossref_primary_10_1101_gr_210005_116 crossref_primary_10_1111_imr_13317 crossref_primary_10_15252_embj_2020105220 crossref_primary_10_1016_j_molcel_2014_04_006 crossref_primary_10_1016_j_cell_2012_09_044 crossref_primary_10_1002_JLB_2A0421_200R crossref_primary_10_1189_jlb_6HI0313_169R crossref_primary_10_1101_gr_260877_120 crossref_primary_10_1002_eji_202250268 crossref_primary_10_1016_j_molmed_2017_02_004 crossref_primary_10_1038_s41467_019_09754_1 crossref_primary_10_1101_gad_253443_114 crossref_primary_10_1111_imr_12208 crossref_primary_10_1126_science_aaf1098 crossref_primary_10_3389_fimmu_2018_02542 crossref_primary_10_1038_nri_2017_51 crossref_primary_10_1016_j_jgg_2020_09_002 crossref_primary_10_1111_imr_12211 crossref_primary_10_1016_j_cell_2016_09_034 crossref_primary_10_1182_blood_2010_08_302976 crossref_primary_10_1038_nrg3207 crossref_primary_10_1111_imr_12213 crossref_primary_10_1093_g3journal_jkad269 crossref_primary_10_1038_s41418_023_01200_6 crossref_primary_10_1016_j_bpj_2021_04_002 crossref_primary_10_1371_journal_pgen_1002401 crossref_primary_10_1038_cr_2016_1 crossref_primary_10_3389_fendo_2023_1272646 crossref_primary_10_1016_j_coi_2011_12_008 crossref_primary_10_3389_fimmu_2018_02219 crossref_primary_10_4161_cc_11_5_19374 crossref_primary_10_4161_trns_2_5_17712 crossref_primary_10_3390_biomedicines9070717 crossref_primary_10_1111_imr_12200 crossref_primary_10_1016_j_bcp_2010_07_029 crossref_primary_10_1126_science_aad5510 crossref_primary_10_1158_0008_5472_CAN_13_0545 crossref_primary_10_1016_j_immuni_2024_03_018 crossref_primary_10_1016_j_smim_2016_03_017 crossref_primary_10_3892_mmr_2017_6504 crossref_primary_10_1089_jir_2016_0004 crossref_primary_10_1371_journal_pgen_1003969 crossref_primary_10_1016_j_smim_2016_03_009 crossref_primary_10_1146_annurev_immunol_020711_075058 crossref_primary_10_1016_j_eswa_2021_115516 crossref_primary_10_1093_nar_gks659 crossref_primary_10_1182_blood_2023020257 crossref_primary_10_1038_nri3777 crossref_primary_10_1084_jem_20180314 crossref_primary_10_53435_funj_00989 crossref_primary_10_1182_blood_2011_03_341123 crossref_primary_10_1172_JCI90604 crossref_primary_10_1074_jbc_M114_575340 crossref_primary_10_1177_0022034520939029 crossref_primary_10_1242_jcs_152314 crossref_primary_10_1186_1471_2105_12_277 crossref_primary_10_3389_fimmu_2021_819029 crossref_primary_10_1158_2326_6066_CIR_13_0117 crossref_primary_10_3389_fimmu_2018_00697 crossref_primary_10_3390_ijms22094647 crossref_primary_10_1016_j_molcel_2010_05_004 crossref_primary_10_1186_s12864_015_1303_0 crossref_primary_10_1101_gad_266999_115 crossref_primary_10_1161_HYPERTENSIONAHA_124_22886 crossref_primary_10_1074_jbc_R111_319046 crossref_primary_10_2217_epi_13_67 crossref_primary_10_1016_j_cmet_2012_02_012 crossref_primary_10_1073_pnas_1016071107 crossref_primary_10_1189_jlb_0312166 crossref_primary_10_1101_gr_152306_112 crossref_primary_10_1016_j_it_2014_03_006 crossref_primary_10_1101_gad_326348_119 crossref_primary_10_1186_s13072_017_0158_9 crossref_primary_10_1016_j_cyto_2018_10_001 crossref_primary_10_1016_j_it_2014_03_001 crossref_primary_10_1080_21541264_2019_1695492 crossref_primary_10_1089_jir_2014_0120 crossref_primary_10_4049_jimmunol_2000624 crossref_primary_10_1093_nar_gkv1475 crossref_primary_10_1016_j_immuni_2012_02_016 crossref_primary_10_1371_journal_pone_0070622 crossref_primary_10_1038_s41590_019_0399_9 crossref_primary_10_2147_ITT_S485672 crossref_primary_10_1016_j_cell_2015_12_023 crossref_primary_10_1002_iid3_184 crossref_primary_10_3390_cells9071620 crossref_primary_10_1093_carcin_bgv016 crossref_primary_10_1016_j_bbagrm_2014_10_006 crossref_primary_10_1146_annurev_genom_090711_163723 crossref_primary_10_1101_gad_351630_124 crossref_primary_10_1101_gad_257592_114 crossref_primary_10_3389_fimmu_2014_00156 crossref_primary_10_1146_annurev_physiol_022516_034348 crossref_primary_10_3389_fimmu_2019_01786 crossref_primary_10_1016_j_virusres_2015_08_019 crossref_primary_10_1016_j_cmet_2018_03_005 crossref_primary_10_1002_cam4_70157 crossref_primary_10_4161_epi_25441 crossref_primary_10_1101_gad_175950_111 crossref_primary_10_1038_leu_2013_260 crossref_primary_10_1093_bib_bby110 crossref_primary_10_1146_annurev_immunol_031210_101303 crossref_primary_10_1371_journal_pone_0093971 crossref_primary_10_1038_ncb2466 crossref_primary_10_1101_gr_136184_111 crossref_primary_10_1155_2018_1312626 crossref_primary_10_4049_jimmunol_1302516 crossref_primary_10_1016_j_immuni_2018_04_024 crossref_primary_10_1016_j_cell_2014_11_023 crossref_primary_10_1038_nrm_2017_113 crossref_primary_10_3389_fimmu_2015_00370 crossref_primary_10_1038_ni_3257 crossref_primary_10_1016_j_molcel_2013_01_038 crossref_primary_10_1016_j_tig_2013_05_008 crossref_primary_10_3389_fimmu_2015_00254 crossref_primary_10_1038_nbt_1946 crossref_primary_10_1007_s12672_024_01623_8 crossref_primary_10_1038_ni_2636 crossref_primary_10_1016_j_cellimm_2020_104187 crossref_primary_10_1093_nar_gks149 crossref_primary_10_3892_mmr_2024_13269 crossref_primary_10_1097_MOL_0000000000000109 crossref_primary_10_4049_jimmunol_2000258 crossref_primary_10_1093_nar_gkt355 crossref_primary_10_7554_eLife_18290 crossref_primary_10_1016_j_biomaterials_2021_121236 crossref_primary_10_1016_j_molcel_2014_06_015 crossref_primary_10_1016_j_devcel_2012_09_003 crossref_primary_10_1111_imr_13036 crossref_primary_10_1038_s41467_019_10652_9 crossref_primary_10_1038_s41467_019_13960_2 crossref_primary_10_1155_2016_6591703 crossref_primary_10_1016_j_cell_2014_11_018 crossref_primary_10_1016_j_cels_2017_01_012 crossref_primary_10_1097_HS9_0000000000000969 crossref_primary_10_1146_annurev_immunol_032414_112032 crossref_primary_10_1371_journal_pone_0150450 crossref_primary_10_1016_j_cell_2013_02_043 crossref_primary_10_4161_cc_10_3_14705 crossref_primary_10_1016_j_coi_2014_04_004 crossref_primary_10_1038_ni_2868 crossref_primary_10_3389_fimmu_2025_1590953 crossref_primary_10_1128_JVI_00422_11 crossref_primary_10_1146_annurev_immunol_093019_123619 crossref_primary_10_1016_j_ceb_2013_07_006 crossref_primary_10_1038_cmi_2016_58 crossref_primary_10_1038_nature12615 crossref_primary_10_1016_j_tig_2011_07_002 crossref_primary_10_1111_j_1600_065X_2012_01103_x crossref_primary_10_1016_j_coph_2013_06_002 crossref_primary_10_1038_nri2958 crossref_primary_10_1016_j_gde_2013_11_017 crossref_primary_10_1038_nri3920 crossref_primary_10_1186_1471_2164_15_120 crossref_primary_10_1101_gr_139360_112 crossref_primary_10_1016_j_bbcan_2019_04_003 crossref_primary_10_1016_j_intimp_2024_113149 crossref_primary_10_1186_s13287_019_1531_3 crossref_primary_10_1038_s41467_023_40937_z crossref_primary_10_4049_jimmunol_2000367 crossref_primary_10_1073_pnas_1819473116 crossref_primary_10_7554_eLife_01503 crossref_primary_10_1093_nar_gku674 crossref_primary_10_1093_nar_gkx821 crossref_primary_10_1038_s41467_020_16209_5 crossref_primary_10_1016_j_immuni_2010_07_006 crossref_primary_10_1016_j_bbadis_2018_10_027 crossref_primary_10_4049_jimmunol_1500204 crossref_primary_10_1016_j_cmet_2016_08_016 crossref_primary_10_1084_jem_20130930 crossref_primary_10_1016_j_imbio_2021_152150 crossref_primary_10_3390_ijms21238943 crossref_primary_10_1002_advs_202307201 crossref_primary_10_1016_j_it_2012_11_001 crossref_primary_10_4049_jimmunol_1102649 crossref_primary_10_1016_j_cell_2012_01_056 crossref_primary_10_1111_imm_13571 crossref_primary_10_1134_S0026893322020054 crossref_primary_10_1038_s41593_018_0079_3 crossref_primary_10_3389_fimmu_2023_1129577 crossref_primary_10_1007_s10238_021_00717_9 crossref_primary_10_1016_j_molcel_2018_07_017 crossref_primary_10_1016_j_jaci_2018_07_014 crossref_primary_10_3389_fimmu_2022_1069207 crossref_primary_10_1016_j_gde_2010_06_010 crossref_primary_10_1016_j_it_2015_07_006 crossref_primary_10_1111_jne_12653 crossref_primary_10_3389_fendo_2020_609099 crossref_primary_10_1016_j_bbi_2011_03_006 crossref_primary_10_1093_nar_gkv331 crossref_primary_10_1016_j_mce_2023_112103 crossref_primary_10_1186_s13075_016_1169_9 crossref_primary_10_1038_nri3702 crossref_primary_10_1038_s41577_019_0141_8 crossref_primary_10_1073_pnas_1121131109 crossref_primary_10_1016_j_immuni_2017_04_016 crossref_primary_10_1016_j_pt_2021_09_009 crossref_primary_10_1016_j_tig_2012_02_008 crossref_primary_10_1016_j_bbadis_2017_08_005 crossref_primary_10_1016_j_cell_2010_09_006 crossref_primary_10_3389_fcell_2021_665897 crossref_primary_10_1101_gad_176826_111 crossref_primary_10_1101_gad_1998010 crossref_primary_10_1242_dev_139139 crossref_primary_10_3389_fimmu_2019_02441 crossref_primary_10_1146_annurev_immunol_032712_100024 crossref_primary_10_1038_nn_3668 crossref_primary_10_1093_nar_gkaf230 crossref_primary_10_1016_j_it_2015_07_005 crossref_primary_10_1111_j_1600_065X_2010_00955_x crossref_primary_10_3389_fimmu_2017_00092 crossref_primary_10_1073_pnas_1820529116 crossref_primary_10_1074_jbc_R111_296491 crossref_primary_10_1111_imm_12115 crossref_primary_10_1002_wsbm_1423 crossref_primary_10_1371_journal_pone_0301904 crossref_primary_10_7554_eLife_34864 crossref_primary_10_1038_ni_3306 crossref_primary_10_1016_j_immuni_2011_08_007 crossref_primary_10_1016_j_bbadis_2010_10_005 crossref_primary_10_1038_s41588_024_01911_7 crossref_primary_10_1189_jlb_1110607 crossref_primary_10_3389_fimmu_2025_1606277 crossref_primary_10_1016_j_mce_2017_07_034 crossref_primary_10_1093_nar_gkw528 crossref_primary_10_3103_S0891416815040114 crossref_primary_10_3390_biomedicines9111596 crossref_primary_10_1083_jcb_201108118 crossref_primary_10_1016_j_cell_2016_01_020 crossref_primary_10_1016_j_lfs_2024_122896 crossref_primary_10_1111_j_1749_6632_2012_06508_x crossref_primary_10_1371_journal_pone_0048102 crossref_primary_10_1002_wsbm_1449 crossref_primary_10_1038_nrrheum_2016_91 crossref_primary_10_1038_s41569_019_0265_3 crossref_primary_10_1080_08830185_2021_1931170 crossref_primary_10_1016_j_molcel_2015_02_029 crossref_primary_10_1371_journal_pone_0024824 crossref_primary_10_1038_icb_2016_82 crossref_primary_10_1016_j_it_2015_02_004 crossref_primary_10_1016_j_bbi_2013_02_005 crossref_primary_10_1016_j_jaut_2018_05_004 crossref_primary_10_1016_j_wneu_2021_06_107 crossref_primary_10_1186_s13059_015_0832_9 crossref_primary_10_1002_glia_22866 crossref_primary_10_1016_j_gde_2023_102146 crossref_primary_10_1134_S0026893311010110 crossref_primary_10_1007_s12026_012_8291_9 crossref_primary_10_1016_j_coi_2014_06_002 crossref_primary_10_1038_s44319_025_00446_0 crossref_primary_10_1126_science_1256271 crossref_primary_10_1186_1471_2164_11_642 crossref_primary_10_1016_j_immuni_2017_07_017 crossref_primary_10_1093_intimm_dxx016 crossref_primary_10_1186_s13072_019_0288_3 crossref_primary_10_1158_0008_5472_CAN_20_4010 crossref_primary_10_3390_biom13060944 crossref_primary_10_1016_j_cell_2012_06_053 crossref_primary_10_1084_jem_20140425 crossref_primary_10_1016_j_immuni_2018_01_012 crossref_primary_10_1007_s00005_011_0147_9 crossref_primary_10_1159_000494070 crossref_primary_10_4049_jimmunol_1502009 crossref_primary_10_1093_nar_gkv267 crossref_primary_10_1038_nri3088 crossref_primary_10_1016_j_immuni_2017_07_012 crossref_primary_10_1002_eji_201141706 crossref_primary_10_1101_gad_291484_116 crossref_primary_10_1073_pnas_1424028112 crossref_primary_10_15252_embr_202153251 crossref_primary_10_1186_1745_6150_6_51 crossref_primary_10_1186_s12864_015_1905_6 crossref_primary_10_1016_j_immuni_2021_05_016 crossref_primary_10_3389_fimmu_2023_1146699 crossref_primary_10_1101_gad_293134_116 crossref_primary_10_1139_o11_042 crossref_primary_10_1186_s12916_015_0293_8 crossref_primary_10_1186_s12974_018_1261_y crossref_primary_10_1038_s41594_025_01633_2 crossref_primary_10_1016_j_bbagrm_2012_10_008 crossref_primary_10_1016_j_immuni_2011_06_002 crossref_primary_10_2217_epi_10_55 crossref_primary_10_1002_art_39135 crossref_primary_10_1016_j_isci_2025_112502 crossref_primary_10_1016_j_smim_2011_08_004 crossref_primary_10_1016_j_pharmthera_2016_07_013 crossref_primary_10_1080_03008207_2016_1208655 crossref_primary_10_1016_j_biopha_2017_07_001 crossref_primary_10_1128_microbiolspec_MCHD_0010_2015 crossref_primary_10_1016_j_it_2014_12_002 crossref_primary_10_1101_gad_2018811 crossref_primary_10_1016_j_gde_2016_11_004 crossref_primary_10_1016_j_tig_2015_10_004 crossref_primary_10_1128_MCB_00836_10 crossref_primary_10_1016_j_cell_2012_12_018 crossref_primary_10_1038_srep28517 crossref_primary_10_1063_5_0087699 crossref_primary_10_1016_j_cyto_2010_12_019 crossref_primary_10_1189_jlb_0211075 crossref_primary_10_1038_s41598_021_94324_z crossref_primary_10_1016_j_lfs_2020_118855 crossref_primary_10_1111_imr_12942 crossref_primary_10_1084_jem_20151764 crossref_primary_10_1089_jir_2015_0138 crossref_primary_10_1186_1471_2164_15_S10_S4 crossref_primary_10_1038_ni_1990 crossref_primary_10_1182_blood_2012_01_402453 crossref_primary_10_1016_j_coph_2015_09_007 crossref_primary_10_1016_j_gpb_2017_02_003 crossref_primary_10_1002_dneu_22545 crossref_primary_10_1016_j_dci_2010_10_003 crossref_primary_10_1038_s41598_017_07100_3 crossref_primary_10_1101_gad_259879_115 crossref_primary_10_1016_j_molcel_2021_08_004 crossref_primary_10_1073_pnas_2322524121 crossref_primary_10_1038_s41594_022_00896_3 crossref_primary_10_1016_j_molimm_2010_05_289 crossref_primary_10_1016_j_molcel_2012_10_013 crossref_primary_10_1016_j_molcel_2015_09_018 crossref_primary_10_4049_jimmunol_1201591 crossref_primary_10_1111_eci_12491 crossref_primary_10_1016_j_immuni_2011_06_013 crossref_primary_10_1161_ATVBAHA_117_310388 crossref_primary_10_1002_JLB_MR0318_126R crossref_primary_10_1146_annurev_biochem_79_081507_103945 crossref_primary_10_1038_cdd_2013_88 crossref_primary_10_1016_j_chom_2018_12_006 crossref_primary_10_1007_s00018_015_1871_9 crossref_primary_10_1016_j_molcel_2017_03_019 crossref_primary_10_1038_nsmb_2153 crossref_primary_10_1016_j_margen_2019_01_003 crossref_primary_10_1017_S0031182022000749 crossref_primary_10_1038_s41467_022_31892_2 crossref_primary_10_1161_CIRCRESAHA_115_306539 crossref_primary_10_3389_fcell_2020_626541 crossref_primary_10_1146_annurev_immunol_25_022106_141623 crossref_primary_10_1007_s00011_014_0711_y crossref_primary_10_1101_gr_211466_116 crossref_primary_10_1093_hmg_ddaa254 crossref_primary_10_1146_annurev_pathmechdis_012418_012718 crossref_primary_10_1002_dneu_22328 crossref_primary_10_1002_hon_2215 crossref_primary_10_1093_intimm_dxae024 crossref_primary_10_15252_embj_2019101533 crossref_primary_10_1038_ni_3818 crossref_primary_10_1093_nar_gkz528 crossref_primary_10_1016_j_gde_2012_06_002 crossref_primary_10_3389_fcimb_2020_00096 crossref_primary_10_1007_s00101_014_2402_z crossref_primary_10_1016_j_cels_2017_03_010 crossref_primary_10_1002_eji_201546237 crossref_primary_10_1186_s13024_019_0347_z |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.immuni.2010.02.008 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1097-4180 |
| ExternalDocumentID | 20206554 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NHGRI NIH HHS grantid: 1R01HG003521-01 |
| GroupedDBID | --- --K -DZ 0R~ 1RT 1~5 2WC 4.4 457 4G. 53G 5GY 62- 7-5 8FE 8FH AAEDT AAEDW AAIKJ AAKRW AALRI AAMRU AAVLU AAXUO AAYWO ABDGV ABJNI ABMAC ABOCM ACGFO ACGFS ACIWK ACPRK ACVFH ADBBV ADCNI ADEZE ADFRT ADVLN AEFWE AENEX AEUPX AEXQZ AFPUW AFRAH AFTJW AGCQF AGGSO AGHFR AGKMS AHHHB AHMBA AIGII AITUG AKAPO AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ APXCP ASPBG AVWKF AZFZN BAWUL BKEYQ BPHCQ BVXVI C45 CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EFKBS EIF EJD F5P FCP FDB FIRID HZ~ IH2 IHE IXB J1W JIG LK8 LX5 M3Z M41 N9A NPM O-L O9- OK1 OVD P2P PQQKQ PROAC RIG ROL RPZ SCP SES SSZ TEORI TR2 7X8 |
| ID | FETCH-LOGICAL-c533t-677f38e94d55260e3c589112d32f6da2ac4dc357dad9624dff6ea2e53c92efbc2 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 537 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000276125400006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1097-4180 |
| IngestDate | Sun Sep 28 06:00:10 EDT 2025 Mon Jul 21 06:04:05 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c533t-677f38e94d55260e3c589112d32f6da2ac4dc357dad9624dff6ea2e53c92efbc2 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://dx.doi.org/10.1016/j.immuni.2010.02.008 |
| PMID | 20206554 |
| PQID | 733838214 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_733838214 pubmed_primary_20206554 |
| PublicationCentury | 2000 |
| PublicationDate | 2010-03-26 |
| PublicationDateYYYYMMDD | 2010-03-26 |
| PublicationDate_xml | – month: 03 year: 2010 text: 2010-03-26 day: 26 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Immunity (Cambridge, Mass.) |
| PublicationTitleAlternate | Immunity |
| PublicationYear | 2010 |
| References | 20346768 - Immunity. 2010 Mar 26;32(3):296-8 |
| References_xml | – reference: 20346768 - Immunity. 2010 Mar 26;32(3):296-8 |
| SSID | ssj0014590 |
| Score | 2.530648 |
| Snippet | Enhancers determine tissue-specific gene expression programs. Enhancers are marked by high histone H3 lysine 4 mono-methylation (H3K4me1) and by the... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 317 |
| SubjectTerms | Animals Binding Sites Cells, Cultured Chromatin - immunology Chromatin - metabolism E1A-Associated p300 Protein - genetics E1A-Associated p300 Protein - metabolism Female Gene Expression Profiling Gene Expression Regulation Inflammation - genetics Inflammation - immunology Inflammation - metabolism Lipopolysaccharides - immunology Macrophages - immunology Macrophages - metabolism Mice Protein Binding Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Regulatory Sequences, Nucleic Acid Trans-Activators - genetics Trans-Activators - metabolism |
| Title | Identification and characterization of enhancers controlling the inflammatory gene expression program in macrophages |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/20206554 https://www.proquest.com/docview/733838214 |
| Volume | 32 |
| WOSCitedRecordID | wos000276125400006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV27TsMwFLWAAmLhUV7lJQ-sFo3t-DEhhKgYaNUBULfK9UNlIC1NQfD3XCdumRADS6YkiuyT62Pfc-9B6FI6bUfKMOI544Rrxcgos5pYZ4MRDEh0JZB9fpC9nhoMdD9pc8okq1zExCpQu4mNZ-RXMu6lFM349fSNRNOomFxNDhqrqMGAyURFlxz8JBF4rutmBFoSnqn2onKukne9VOUXSdsVm3aq3zlmtdZ0dv75lbtoO5FMfFOjYg-t-KKJNmrbya8m2uymhPo-SoW6IZ3cYVM4bJc9nOsSTTwJ2BfjCI9ZiZO2PVaxYyCPGBAKoHqtkvUY0Oix_0zi2gIn9RfchF9NNAsbQ_gqD9BT5-7x9p4kIwZigQ3OiZAyMOU1d3kO-x_PbPQizKhjNAhnqLHcWZZLZ5wWlLsQhDfU58xq6sPI0kO0VkwKf4yw07mXueHWwbooRFuN4MXtkCufeQ4z1kJ4MbBDAHrMXpjCT97L4XJoW-ionpzhtG7IMaTAeQXwopO_Hz5FW3X-nxEqzlAjwE_uz9G6_Zi_lLOLCkBw7fW733sb0xY |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+and+characterization+of+enhancers+controlling+the+inflammatory+gene+expression+program+in+macrophages&rft.jtitle=Immunity+%28Cambridge%2C+Mass.%29&rft.au=Ghisletti%2C+Serena&rft.au=Barozzi%2C+Iros&rft.au=Mietton%2C+Flore&rft.au=Polletti%2C+Sara&rft.date=2010-03-26&rft.issn=1097-4180&rft.eissn=1097-4180&rft.volume=32&rft.issue=3&rft.spage=317&rft_id=info:doi/10.1016%2Fj.immuni.2010.02.008&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-4180&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-4180&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-4180&client=summon |