Fungal Alpha-Galactosidase from Pseudobalsamia microspora Capable of Degrading Raffinose Family Oligosaccharides

An alpha-galactosidase was purified from Pseudobalsamia microspora (PMG) to 1224.1-fold with a specific activity of 11,274.5 units/mg by ion-exchange chromatography and gel filtration. PMG is a monomeric protein with a molecular mass of 62 kDa as determined by SDS-PAGE and by gel filtration. Chemica...

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Veröffentlicht in:	Applied biochemistry and biotechnology Jg. 176; H. 8; S. 2157 - 2169
Hauptverfasser:	Yang, Dongxue, Tian, Guoting, Du, Fang, Zhao, Yongchang, Zhao, Liyan, Wang, Hexiang, Ng, Tzi Bun
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Springer US 01.08.2015 Springer Nature B.V
Schlagworte:	alpha-galactosidase alpha-Galactosidase - chemistry alpha-Galactosidase - isolation & purification alpha-Galactosidase - metabolism Amino Acid Sequence Amino acids Bacteria Biochemistry Biotechnology cadmium Chemistry Chemistry and Materials Science chromatography Chromatography, Ion Exchange Chromatography, Thin Layer copper Electrophoresis, Polyacrylamide Gel essential amino acids Feed industry Filtration Fungi gel chromatography Hydrogen-Ion Concentration Hydrolysis Indicators and Reagents ion exchange chromatography Ions iron Kinetics Liquid chromatography Mass spectrometry mercury Metals - pharmacology Microspora Microsporidia - enzymology Microsporidia - isolation & purification Molecular Sequence Data Molecular Weight Mushrooms polyacrylamide gel electrophoresis raffinose Raffinose - metabolism stachyose Substrate Specificity - drug effects tandem mass spectrometry Temperature Thin layer chromatography Degradation Purification and characterization Chemical modification Alpha-galactosidase
ISSN:	0273-2289, 1559-0291, 1559-0291
Online-Zugang:	Volltext
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