Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence
Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacki...
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| Veröffentlicht in: | Molecular plant-microbe interactions Jg. 24; H. 10; S. 1132 - 1142 |
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| Sprache: | Englisch |
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United States
The American Phytopathological Society
01.10.2011
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| ISSN: | 0894-0282, 1943-7706 |
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| Abstract | Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans. |
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| AbstractList | Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans. Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans. |
| Author | Jacobsen, Evert Li, Guangcun Rietman, Hendrik Yang, Yu Vleeshouwers, Vivianne G G A Guo, Xiao Visser, Richard G F Qu, Dongyu Huang, Sanwen Bergervoet, Marjan Li, Ying Guo, Zhen van der Vossen, Edwin A G Kuang, Hanhui Vossen, Jack H |
| Author_xml | – sequence: 1 givenname: Guangcun surname: Li fullname: Li, Guangcun organization: key Laboratory of Corp Genetic Improvement and Biotechnology, Shandong Province, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R. China – sequence: 2 givenname: Sanwen surname: Huang fullname: Huang, Sanwen – sequence: 3 givenname: Xiao surname: Guo fullname: Guo, Xiao – sequence: 4 givenname: Ying surname: Li fullname: Li, Ying – sequence: 5 givenname: Yu surname: Yang fullname: Yang, Yu – sequence: 6 givenname: Zhen surname: Guo fullname: Guo, Zhen – sequence: 7 givenname: Hanhui surname: Kuang fullname: Kuang, Hanhui – sequence: 8 givenname: Hendrik surname: Rietman fullname: Rietman, Hendrik – sequence: 9 givenname: Marjan surname: Bergervoet fullname: Bergervoet, Marjan – sequence: 10 givenname: Vivianne G G A surname: Vleeshouwers fullname: Vleeshouwers, Vivianne G G A – sequence: 11 givenname: Edwin A G surname: van der Vossen fullname: van der Vossen, Edwin A G – sequence: 12 givenname: Dongyu surname: Qu fullname: Qu, Dongyu – sequence: 13 givenname: Richard G F surname: Visser fullname: Visser, Richard G F – sequence: 14 givenname: Evert surname: Jacobsen fullname: Jacobsen, Evert – sequence: 15 givenname: Jack H surname: Vossen fullname: Vossen, Jack H |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21649512$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Agrobacterium tumefaciens - genetics Base Sequence Chromosome Mapping Chromosomes, Plant - genetics Cloning, Molecular DNA, Plant - genetics Genes, Plant Genetic Complementation Test Host-Pathogen Interactions - genetics Multigene Family Nicotiana - genetics Nicotiana - microbiology Phylogeny Phytophthora infestans - pathogenicity Plant Diseases - genetics Plant Diseases - microbiology Plant Diseases - prevention & control Plant Leaves - microbiology Plants, Genetically Modified Solanum tuberosum - genetics Solanum tuberosum - microbiology Transformation, Genetic Virulence |
| Title | Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence |
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