Immunohistochemical characterization of endometrial leucocytes in endometritis

Aims:  Leucocytes are a normal and variable component of the endometrial stromal cell population. The aim of this study was to characterize endometrial leucocytes in established cases of endometritis in order to determine whether there are objective characteristics of the leucocyte infiltrate which...

Full description

Saved in:
Bibliographic Details
Published in:Histopathology Vol. 45; no. 6; pp. 625 - 632
Main Authors: Disep, B, Innes, B A, Cochrane, H R, Tijani, S, Bulmer, J N
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Science Ltd 01.12.2004
Blackwell
Subjects:
Antigens, CD - analysis
Antigens, CD20 - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Bacterial diseases
Bacterial diseases of the genital system
Biological and medical sciences
CD3 Complex - analysis
CD56 Antigen - analysis
Cell Count
endometrial leucocytes
endometritis
Endometritis - metabolism
Endometritis - pathology
Endometrium - pathology
Female
Human bacterial diseases
human endometrium
Humans
Immunohistochemistry
Infectious diseases
Investigative techniques, diagnostic techniques (general aspects)
Leukocyte Common Antigens - analysis
Leukocytes - chemistry
Leukocytes - pathology
Leukosialin
Macrophages - chemistry
Macrophages - pathology
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Sialoglycoproteins - analysis
T-Lymphocytes - chemistry
T-Lymphocytes - pathology
Characterization
Human
Immunohistochemistry
Anatomic pathology
Leukocyte
endometrial leucocytes
Female genital system
human endometrium
Uterine diseases
Endometritis
Endometrium
Female genital diseases
ISSN:0309-0167, 1365-2559
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aims:  Leucocytes are a normal and variable component of the endometrial stromal cell population. The aim of this study was to characterize endometrial leucocytes in established cases of endometritis in order to determine whether there are objective characteristics of the leucocyte infiltrate which would allow its identification as part of an inflammatory process rather then the normal physiological leucocyte infiltrate. Methods and results:  We examined endometrial tissue from 79 cases of endometritis and 22 histologically normal controls. Leucocytes were characterized immunohistochemically for CD45, CD20, CD68, CD3 and CD56 and numbers were analysed semiquantitatively on a scale of 0–4. In many endometritis cases the overall number of leucocytes was increased. Furthermore, leucocytes were unusually distributed with a tendency to accumulate superficially beneath the endometrial surface. Whilst numbers of macrophages, T lymphocytes and endometrial granulated lymphocytes (uterine natural killer cells) did not differ between endometritis samples and controls, most endometritis cases contained a substantially increased number of B cells, which normally represent 1% or less of the endometrial leucocyte population. B lymphocytes were also observed in unusual locations such as intraepithelially and within glandular lumina. Conclusions:  These results suggest that immunohistochemical characterization of endometrial leucocytes may be helpful in establishing a diagnosis of endometritis in equivocal cases.
Bibliography:ark:/67375/WNG-V7LN1CNC-P
ArticleID:HIS2052
istex:DF25E18834829D4B94612BC09B5EC336A9883EDC
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0309-0167
1365-2559
DOI:10.1111/j.1365-2559.2004.02052.x